DNASU Plasmid Repository • 480.965.5697 | Email

SCML1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  SCML1
Gene Name:  sex comb on midleg-like 1 (Drosophila)
Sequence             
Original Clone ID: FLH268446.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329713

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 627nts         Open reading frame : 1 to 627
Reference Sequence Alignment

ATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCCACTTTA
CCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTTTGCATG
GAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCGAGTCCA
GTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGATGGTGCA
ACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATCCACAAC
ACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCAGTTGAA
AATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCGGTGGAA
GCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTCGACCTC
TTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTGTTGCTG
AAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGACCGACTT
AAACAAGGAAAATGCTTTGAAAATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1242
Start on reference sequence 616
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SCML1
Symbol Nomenclature : SCML1
Designation : sex comb on midleg-like protein 1
Full Nomenclature : sex comb on midleg-like 1 (Drosophila)
GENEID : 6322
Locus Tag : RP3-389A20.1
HGNC : 10580
MIM : 300227
Vega : OTTHUMG00000021206
Target GenBank: BC026159

 

Reference Sequence Alignment

  

HsCD00329713        ------------------------------------------------------------
XM_005274578.1      ATGATGTCTAACAGCTCCAGTGAAATCGATGTGATAAAAACAAGAATACCTACTTACGAT
NM_001037536.2      ------------------------------------------------------------
NM_006746.5         ATGATGTCTAACAGCTCCAGTGAAATCGAT------------------------------
NM_001037535.2      ------------------------------------------------------------
XM_005274579.1      ATGATGTCTAACAGCTCCAGTGAAATCGATGTGG--------------------------
NM_001037540.2      ATGATGTCTAACAGCTCCAGTGAAATCGATGTGATAAAAACAAGAATACCTACTTACGAT
                                                                                

HsCD00329713        ------------------------------------------------------------
XM_005274578.1      GAAGATGACAACACTATTCTTTATGCGTATGAAACAAAACCTGAATTTGTCAATAAACAG
NM_001037536.2      ------------------------------------------------------------
NM_006746.5         ------------------------------------------------------GTGCAG
NM_001037535.2      ------------------------------------------------------------
XM_005274579.1      ----------------------------------------------------------AA
NM_001037540.2      GAAGATGACAACACTATTCTTTATGCGTATGAAACAAAACCTGAATTTGTCAATAAAGAA
                                                                                

HsCD00329713        ------------------------------------------------------------
XM_005274578.1      GAACCGAATATTGTATCTGACGCATCCTGTAATACTGAAGAGCAACTGAAGACAGTTGAT
NM_001037536.2      ------------------------------------------------------------
NM_006746.5         GAACCGAATATTGTATCTGACGCATCCTGTAATACTGAAGAGCAACTGAAGACAGTTGAT
NM_001037535.2      ------------------------------------------------------------
XM_005274579.1      CCGAA---TATTGTATCTGACGCATCCTGTAATACTGAAGAGCAACTGAAGACAGTTGAT
NM_001037540.2      CCGAA---TATTGTATCTGACGCATCCTGTAATACTGAAGAGCAACTGAAGACAGTTGAT
                                                                                

HsCD00329713        ------------------------------------------------------------
XM_005274578.1      GATGTCCTTATTCATTGCCAGGTTATATATGATGCTCTGCAAAACCTGGATAAGAAGATT
NM_001037536.2      ------------------------------------------------------------
NM_006746.5         GATGTCCTTATTCATTGCCAGGTTATATATGATGCTCTGCAAAACCTGGATAAGAAGATT
NM_001037535.2      ------------------------------------------------------------
XM_005274579.1      GATGTCCTTATTCATTGCCAGGTTATATATGATGCTCTGCAAAACCTGGATAAGAAGATT
NM_001037540.2      GATGTCCTTATTCATTGCCAGGTTATATATGATGCTCTGCAAAACCTGGATAAGAAGATT
                                                                                

HsCD00329713        ------------------------------------------------------------
XM_005274578.1      GATGTGATTCGTAGAAAGGTTTCAAAAATCCAACGTTTCCATGCGAGATCCCTGTGGACA
NM_001037536.2      ------------------------------------------------------------
NM_006746.5         GATGTGATTCGTAGAAAGGTTTCAAAAATCCAACGTTTCCATGCGAGATCCCTGTGGACA
NM_001037535.2      ------------------------------------------------------------
XM_005274579.1      GATGTGATTCGTAGAAAGGTTTCAAAAATCCAACGTTTCCATGCGAGATCCCTGTGGACA
NM_001037540.2      GATGTGATTCGTAGAAAGGTTTCAAAAATCCAACGTTTCCATGCGAGATCCCTGTGGACA
                                                                                

HsCD00329713        ------------------------------------------------------------
XM_005274578.1      AATCATAAGCGATATGGATATAAAAAGCATTCTTACCGGCTTGTTAAAAAGCTTAAACTC
NM_001037536.2      ------------------------------------------------------------
NM_006746.5         AATCATAAGCGATATGGATATAAAAAGCATTCTTACCGGCTTGTTAAAAAGCTTAAACTC
NM_001037535.2      ------------------------------------------------------------
XM_005274579.1      AATCATAAGCGATATGGATATAAAAAGCATTCTTACCGGCTTGTTAAAAAGCTTAAACTC
NM_001037540.2      AATCATAAGCGATATGGATATAAAAAGCATTCTTACCGGCTTGTTAAAAAGCTTAAACTC
                                                                                

HsCD00329713        ------ATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCC
XM_005274578.1      CAGAAAATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCC
NM_001037536.2      ------ATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCC
NM_006746.5         CAGAAAATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCC
NM_001037535.2      ------ATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCC
XM_005274579.1      CAGAAAATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCC
NM_001037540.2      CAGAAAATGAAGAAAAATGAGGTTTACGAGACATTCTCCTACCCTGAAAGTTACAGCCCC
                          ******************************************************

HsCD00329713        ACTTTACCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTT
XM_005274578.1      ACTTTACCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTT
NM_001037536.2      ACTTTACCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTT
NM_006746.5         ACTTTACCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTT
NM_001037535.2      ACTTTACCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTT
XM_005274579.1      ACTTTACCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTT
NM_001037540.2      ACTTTACCAGTGTCAAGGCGTGAGAATAATTCCCCGAGCAACCTTCCAAGGCCATCCTTT
                    ************************************************************

HsCD00329713        TGCATGGAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCG
XM_005274578.1      TGCATGGAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCG
NM_001037536.2      TGCATGGAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCG
NM_006746.5         TGCATGGAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCG
NM_001037535.2      TGCATGGAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCG
XM_005274579.1      TGCATGGAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCG
NM_001037540.2      TGCATGGAAGAATACCAGCGAGCTGAGCTGGAGGAGGACCCGATCCTCAGCCGCACTCCG
                    ************************************************************

HsCD00329713        AGTCCAGTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGAT
XM_005274578.1      AGTCCAGTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGAT
NM_001037536.2      AGTCCAGTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGAT
NM_006746.5         AGTCCAGTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGAT
NM_001037535.2      AGTCCAGTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGAT
XM_005274579.1      AGTCCAGTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGAT
NM_001037540.2      AGTCCAGTGCATCCCTCAGATTTCTCTGAGCATAATTGTCAGCCGTATTATGCATCTGAT
                    ************************************************************

HsCD00329713        GGTGCAACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATC
XM_005274578.1      GGTGCAACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATC
NM_001037536.2      GGTGCAACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATC
NM_006746.5         GGTGCAACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATC
NM_001037535.2      GGTGCAACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATC
XM_005274579.1      GGTGCAACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATC
NM_001037540.2      GGTGCAACGTATGGTTCTTCTTCAGGGCTCTGCCTTGGCAACCCTCGGGCTGACAGCATC
                    ************************************************************

HsCD00329713        CACAACACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCA
XM_005274578.1      CACAACACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCA
NM_001037536.2      CACAACACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCA
NM_006746.5         CACAACACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCA
NM_001037535.2      CACAACACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCA
XM_005274579.1      CACAACACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCA
NM_001037540.2      CACAACACTTACTCAACTGACCATGCTTCTGCAGCACCACCTTCAGTTACAAGGTCACCA
                    ************************************************************

HsCD00329713        GTTGAAAATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCG
XM_005274578.1      GTTGAAAATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCG
NM_001037536.2      GTTGAAAATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCG
NM_006746.5         GTTGAAAATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCG
NM_001037535.2      GTTGAAAATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCG
XM_005274579.1      GTTGAAAATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCG
NM_001037540.2      GTTGAAAATGACGGTTACATAGAGGAAGGAAGCATCACTAAGCACCCTTCAACCTGGTCG
                    ************************************************************

HsCD00329713        GTGGAAGCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTC
XM_005274578.1      GTGGAAGCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTC
NM_001037536.2      GTGGAAGCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTC
NM_006746.5         GTGGAAGCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTC
NM_001037535.2      GTGGAAGCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTC
XM_005274579.1      GTGGAAGCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTC
NM_001037540.2      GTGGAAGCAGTGGTCCTATTTCTAAAACAAACAGATCCTCTTGCATTATGCCCTCTTGTC
                    ************************************************************

HsCD00329713        GACCTCTTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTG
XM_005274578.1      GACCTCTTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTG
NM_001037536.2      GACCTCTTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTG
NM_006746.5         GACCTCTTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTG
NM_001037535.2      GACCTCTTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTG
XM_005274579.1      GACCTCTTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTG
NM_001037540.2      GACCTCTTCAGAAGCCATGAAATTGACGGGAAGGCTCTGCTCCTACTCACGAGTGACGTG
                    ************************************************************

HsCD00329713        TTGCTGAAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGAC
XM_005274578.1      TTGCTGAAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGAC
NM_001037536.2      TTGCTGAAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGAC
NM_006746.5         TTGCTGAAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGAC
NM_001037535.2      TTGCTGAAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGAC
XM_005274579.1      TTGCTGAAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGAC
NM_001037540.2      TTGCTGAAGCACTTGGGGGTGAAGCTGGGAACGGCTGTGAAGCTATGCTACTACATTGAC
                    ************************************************************

HsCD00329713        CGACTTAAACAAGGAAAATGCTTTGAAAATTTG
XM_005274578.1      CGACTTAAACAAGGAAAATGCTTTGAAAATTGA
NM_001037536.2      CGACTTAAACAAGGAAAATGCTTTGAAAATTGA
NM_006746.5         CGACTTAAACAAGGAAAATGCTTTGAAAATTGA
NM_001037535.2      CGACTTAAACAAGGAAAATGCTTTGAAAATTGA
XM_005274579.1      CGACTTAAACAAGGAAAATGCTTTGAAAATTGA
NM_001037540.2      CGACTTAAACAAGGAAAATGCTTTGAAAATTGA
                    ******************************* .

Protein

SMART domain : SAM : Sterile alpha motif.
UniProt : Q9UN30
HPRD : 02205

Clone

Cloning Information : 268446
HIP Master Clone ID : 193463
Original Clone ID : FLH268446.01L

Organism


TAX_ID : 9606
Species Specific ID: 6322

Genome

Chromosome : X
Map Location : Xp22
Ensembl : ENSG00000047634

Reagents

Labome : SCML1-antibody