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LOC51058 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  ZNF691
Gene Name:  zinc finger protein 691
Sequence             
Original Clone ID: FLH268457.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329724

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 855nts         Open reading frame : 1 to 855
Reference Sequence Alignment

ATGGGCAGTGAGAAGGAGCAGAGTCCAGAACCACACCTGCCTGAGGAAGGGGAAGGGGGT
AAGCCTTGGAGAGTGGATGACTCAGAGGGTTCTTGGATCCCACCTGGGGAGAAGGAGCAT
GGGCAAGAGAGCCTGTCGGATGAACTGCAAGAAACTCATCCAAAAAAGCCATGGCAGAAA
GTCACTGTCCGGGCTCGAGAGCTAGGGGACCCCATTGCTCATCCAAGGCATGAGGCAGAT
GAGAAGCCCTTTATATGTGCCCAGTGTGGCAAAACCTTCAATAATACCTCCAACCTGAGA
ACACACCAGCGGATCCACACTGGTGAGAAGCCTTACAAGTGTTCTGAATGTGGCAAGAGC
TTCTCGAGAAGCTCCAACCGCATCCGGCACGAGCGGATCCACCTGGAAGAGAAACACTAC
AAATGCCCCAAGTGCCAGGAGAGCTTTCGGCGGCGCTCAGACCTCACCACGCACCAGCAA
GATCACCTAGGCAAGCGGCCATACCGCTGTGACATCTGTGGCAAGAGCTTCAGCCAGAGT
GCCACGCTAGCTGTGCATCACCGGACCCACCTGGAGCCAGCACCCTACATCTGCTGTGAG
TGTGGGAAGAGCTTCAGCAACAGCTCCAGCTTTGGCGTGCATCACCGCACCCACACAGGT
GAGAGACCTTATGAGTGCACTGAGTGTGGGCGGACCTTCAGCGATATCTCCAACTTTGGA
GCACACCAGCGGACCCACAGAGGGGAGAAGCCCTACCGGTGCACTGTGTGTGGGAAACAC
TTCTCCCGGAGCTCGAATCTCATCCGCCACCAGAAAACTCACTTGGGCGAACAGGCTGGG
AAAGATTCCAGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 992
Start on reference sequence 138
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF691
Symbol Nomenclature : ZNF691
SYNONYM : Zfp691
Full Nomenclature : zinc finger protein 691
GENEID : 51058
Locus Tag : RP11-342M1.5
HGNC : 28028
Vega : OTTHUMG00000007623
Target GenBank: BC000157

 

Reference Sequence Alignment

  

HsCD00329724        ------------------------------------------------------------
NM_015911.3         ------------------------------------------------------------
NM_001242739.1      ATGTCACTCTGTTCACCAACCCACTCTGCTGAAATGTCGTTATTTCTTCAAGGCCCGGAG
                                                                                

HsCD00329724        ---------------------------------ATGGGCAGTGAGAAGGAGCAGAGTCCA
NM_015911.3         ---------------------------------ATGGGCAGTGAGAAGGAGCAGAGTCCA
NM_001242739.1      GAAATGCTACCACTCTCATCAGAGGGTTCAGAGATGGGCAGTGAGAAGGAGCAGAGTCCA
                                                     ***************************

HsCD00329724        GAACCACACCTGCCTGAGGAAGGGGAAGGGGGTAAGCCTTGGAGAGTGGATGACTCAGAG
NM_015911.3         GAACCACACCTGCCTGAGGAAGGGGAAGGGGGTAAGCCTTGGAGAGTGGATGACTCAGAG
NM_001242739.1      GAACCACACCTGCCTGAGGAAGGGGAAGGGGGTAAGCCTTGGAGAGTGGATGACTCAGAG
                    ************************************************************

HsCD00329724        GGTTCTTGGATCCCACCTGGGGAGAAGGAGCATGGGCAAGAGAGCCTGTCGGATGAACTG
NM_015911.3         GGTTCTTGGATCCCACCTGGGGAGAAGGAGCATGGGCAAGAGAGCCTGTCGGATGAACTG
NM_001242739.1      GGTTCTTGGATCCCACCTGGGGAGAAGGAGCATGGGCAAGAGAGCCTGTCGGATGAACTG
                    ************************************************************

HsCD00329724        CAAGAAACTCATCCAAAAAAGCCATGGCAGAAAGTCACTGTCCGGGCTCGAGAGCTAGGG
NM_015911.3         CAAGAAACTCATCCAAAAAAGCCATGGCAGAAAGTCACTGTCCGGGCTCGAGAGCTAGGG
NM_001242739.1      CAAGAAACTCATCCAAAAAAGCCATGGCAGAAAGTCACTGTCCGGGCTCGAGAGCTAGGG
                    ************************************************************

HsCD00329724        GACCCCATTGCTCATCCAAGGCATGAGGCAGATGAGAAGCCCTTTATATGTGCCCAGTGT
NM_015911.3         GACCCCATTGCTCATCCAAGGCATGAGGCAGATGAGAAGCCCTTTATATGTGCCCAGTGT
NM_001242739.1      GACCCCATTGCTCATCCAAGGCATGAGGCAGATGAGAAGCCCTTTATATGTGCCCAGTGT
                    ************************************************************

HsCD00329724        GGCAAAACCTTCAATAATACCTCCAACCTGAGAACACACCAGCGGATCCACACTGGTGAG
NM_015911.3         GGCAAAACCTTCAATAATACCTCCAACCTGAGAACACACCAGCGGATCCACACTGGTGAG
NM_001242739.1      GGCAAAACCTTCAATAATACCTCCAACCTGAGAACACACCAGCGGATCCACACTGGTGAG
                    ************************************************************

HsCD00329724        AAGCCTTACAAGTGTTCTGAATGTGGCAAGAGCTTCTCGAGAAGCTCCAACCGCATCCGG
NM_015911.3         AAGCCTTACAAGTGTTCTGAATGTGGCAAGAGCTTCTCGAGAAGCTCCAACCGCATCCGG
NM_001242739.1      AAGCCTTACAAGTGTTCTGAATGTGGCAAGAGCTTCTCGAGAAGCTCCAACCGCATCCGG
                    ************************************************************

HsCD00329724        CACGAGCGGATCCACCTGGAAGAGAAACACTACAAATGCCCCAAGTGCCAGGAGAGCTTT
NM_015911.3         CACGAGCGGATCCACCTGGAAGAGAAACACTACAAATGCCCCAAGTGCCAGGAGAGCTTT
NM_001242739.1      CACGAGCGGATCCACCTGGAAGAGAAACACTACAAATGCCCCAAGTGCCAGGAGAGCTTT
                    ************************************************************

HsCD00329724        CGGCGGCGCTCAGACCTCACCACGCACCAGCAAGATCACCTAGGCAAGCGGCCATACCGC
NM_015911.3         CGGCGGCGCTCAGACCTCACCACGCACCAGCAAGATCACCTAGGCAAGCGGCCATACCGC
NM_001242739.1      CGGCGGCGCTCAGACCTCACCACGCACCAGCAAGATCACCTAGGCAAGCGGCCATACCGC
                    ************************************************************

HsCD00329724        TGTGACATCTGTGGCAAGAGCTTCAGCCAGAGTGCCACGCTAGCTGTGCATCACCGGACC
NM_015911.3         TGTGACATCTGTGGCAAGAGCTTCAGCCAGAGTGCCACGCTAGCTGTGCATCACCGGACC
NM_001242739.1      TGTGACATCTGTGGCAAGAGCTTCAGCCAGAGTGCCACGCTAGCTGTGCATCACCGGACC
                    ************************************************************

HsCD00329724        CACCTGGAGCCAGCACCCTACATCTGCTGTGAGTGTGGGAAGAGCTTCAGCAACAGCTCC
NM_015911.3         CACCTGGAGCCAGCACCCTACATCTGCTGTGAGTGTGGGAAGAGCTTCAGCAACAGCTCC
NM_001242739.1      CACCTGGAGCCAGCACCCTACATCTGCTGTGAGTGTGGGAAGAGCTTCAGCAACAGCTCC
                    ************************************************************

HsCD00329724        AGCTTTGGCGTGCATCACCGCACCCACACAGGTGAGAGACCTTATGAGTGCACTGAGTGT
NM_015911.3         AGCTTTGGCGTGCATCACCGCACCCACACAGGTGAGAGACCTTATGAGTGCACTGAGTGT
NM_001242739.1      AGCTTTGGCGTGCATCACCGCACCCACACAGGTGAGAGACCTTATGAGTGCACTGAGTGT
                    ************************************************************

HsCD00329724        GGGCGGACCTTCAGCGATATCTCCAACTTTGGAGCACACCAGCGGACCCACAGAGGGGAG
NM_015911.3         GGGCGGACCTTCAGCGATATCTCCAACTTTGGAGCACACCAGCGGACCCACAGAGGGGAG
NM_001242739.1      GGGCGGACCTTCAGCGATATCTCCAACTTTGGAGCACACCAGCGGACCCACAGAGGGGAG
                    ************************************************************

HsCD00329724        AAGCCCTACCGGTGCACTGTGTGTGGGAAACACTTCTCCCGGAGCTCGAATCTCATCCGC
NM_015911.3         AAGCCCTACCGGTGCACTGTGTGTGGGAAACACTTCTCCCGGAGCTCGAATCTCATCCGC
NM_001242739.1      AAGCCCTACCGGTGCACTGTGTGTGGGAAACACTTCTCCCGGAGCTCGAATCTCATCCGC
                    ************************************************************

HsCD00329724        CACCAGAAAACTCACTTGGGCGAACAGGCTGGGAAAGATTCCAGCTTG
NM_015911.3         CACCAGAAAACTCACTTGGGCGAACAGGCTGGGAAAGATTCCAGCTGA
NM_001242739.1      CACCAGAAAACTCACTTGGGCGAACAGGCTGGGAAAGATTCCAGCTGA
                    ********************************************** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q5VV52
HPRD : 14229

Clone

Cloning Information : 268457
HIP Master Clone ID : 193479
Original Clone ID : FLH268457.01L

Organism


TAX_ID : 9606
Species Specific ID: 51058

Genome

Chromosome : 1
Map Location : 1p34.2
Ensembl : ENSG00000164011

Reagents

Labome : ZNF691-antibody