DNASU Plasmid Repository • 480.965.5697 | Email

TULP1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TULP1
Gene Name:  tubby like protein 1
Sequence             
Original Clone ID: FLH268495.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329754

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1470nts         Open reading frame : 1 to 1470
Reference Sequence Alignment

ATGCCTCTGCGGGATGAAACCCTCCGAGAGGTGTGGGCCTCTGACAGTGGGCATGAAGAA
GAAAGCCTGAGCCCGGAGGCCCCGCGGCGCCCCAAACAGCGACCCGCCCCGGCACAGAGG
CTAAGGAAGAAGAGGACGGAGGCCCCCGAATCCCCCTGCCCCACGGGATCCAAGCCCCGG
AAGCCCGGAGAGGAGGAGGAGGAAGAGGAGGACGAGGAGGACGAGGAAGAGGAGGCAGAG
GAAAAGAAAGAGAAAATCCTTCTGCCTCCCAAGAAGCCCCTGAGAGAGAAGAGCTCCGCA
GACCTGAAGGAGAGGAGGGCCAAGGCCCAGGGCCCAAGGGGAGACCTGGGAAGCCCTGAC
CCCCCACCGAAACCTCTGCGTGTTAGGAATAAGGAAGCTCCAGCAGGGGAGGGGACCAAG
ATGAGAAAGACCAAGAAGAAAGGGTCTGGGGAGGCCGACAAGGACCCCTCAGGGAGCCCA
GCCAGTGCGAGGAAGAGCCCAGCAGCCATGTTTCTGGTTGGGGAAGGCAGTCCTGACAAG
AAAGCCCTGAAGAAGAAAGGCACTCCCAAAGGCGCGAGGAAGGAGGAAGAAGAGGAGGAG
GAGGCAGCTACGGTGATAAAGAACAGCAATCAAAAGGGCAAAGCCAAAGGAAAAGGCAAA
AAGAAAGCGAAGGAGGAGAGGGCCCCGTCTCCCCCCGTGGAGGTGGACGAACCCCGGGAG
TTTGTGCTCCGGCCTGCCCCCCAGGGCCGCACGGTGCGCTGCCGGCTGACCCGGGACAAA
AAGGGCATGGATCGAGGCATGTATCCCTCCTACTTCCTGCACCTGGACACGGAGAAGAAG
GTGTTCCTCTTGGCTGGCAGGAAACGAAAACGGAGCAAGACAGCCAATTACCTCATCTCC
ATCGACCCTACCAATCTGTCCCGAGGAGGGGAGAATTTCATCGGGAAGCTGAGGTCCAAC
CTCCTGGGGAACCGCTTCACGGTCTTTGACAACGGGCAGAACCCACAGCGTGGGTACAGC
ACTAATGTGGCAAGCCTTCGGCAGGAGCTGGCAGCTGTGATCTATGAAACCAACGTGCTG
GGCTTCCGTGGCCCCCGGCGCATGACCGTCATCATTCCTGGCATGAGTGCGGAGAACGAG
AGGGTCCCCATCCGGCCCCGAAATGCTAGTGACGGCCTGCTGGTGCGCTGGCAGAACAAG
ACGCTGGAGAGCCTCATAGAACTGCACAACAAGCCACCTGTCTGGAACGATGACAGTGGC
TCCTACACCCTCAACTTCCAAGGCCGGGTCACCCAGGCCTCAGTCAAGAACTTCCAGATT
GTCCACGCTGATGACCCCGACTATATCGTGCTGCAGTTCGGCCGCGTGGCGGAGGACGCC
TTCACCCTAGACTACCGGTACCCGCTGTGCGCCCTGCAGGCCTTCGCCATCGCCCTCTCC
AGTTTCGACGGGAAGCTGGCCTGCGAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1482
Start on reference sequence 13
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TULP1
Symbol Nomenclature : TULP1
SYNONYM : LCA15; RP14; TUBL1
Designation : tubby-related protein 1
Full Nomenclature : tubby like protein 1
GENEID : 7287
Locus Tag : RP3-340B19.1
HGNC : 12423
MIM : 602280
Vega : OTTHUMG00000014575
Target GenBank: BC065261

 

Reference Sequence Alignment

  

HsCD00329754        ATGCCTCTGCGGGATGAAACCCTCCGAGAGGTGTGGGCCTCTGACAGTGGGCATGAAGAA
NM_003322.4         ATGCCTCTGCGGGATGAAACCCTCCGAGAGGTGTGGGCCTCTGACAGTGGGCATGAAGAA
NM_001289395.1      ATGCCTCTGCGGGATGAAACCCTCCGAGAGGTGTGGGCCTCTGACAGTGGGCATGAAGAA
                    ************************************************************

HsCD00329754        GAAAGCCTGAGCCCGGAGGCCCCGCGGCGCCCCAAACAGCGACCCGCCCCGGCACAGAGG
NM_003322.4         GAAAGCCTGAGCCCGGAGGCCCCGCGGCGCCCCAAACAGCGACCCGCCCCGGCACAGAGG
NM_001289395.1      GAAAGCCTGAGCCCGGAGGCCCCGCGGCGCCCCAAACAGCGACCCGCCCCGGCACAGAGG
                    ************************************************************

HsCD00329754        CTAAGGAAGAAGAGGACGGAGGCCCCCGAATCCCCCTGCCCCACGGGATCCAAGCCCCGG
NM_003322.4         CTAAGGAAGAAGAGGACGGAGGCCCCCGAATCCCCCTGCCCCACGGGATCCAAGCCCCGG
NM_001289395.1      CTAAGGAAGAAGAGGACGGAGGCCCCCGAATCCCCCTGCCCCACGGGATCCAAGCCCCGG
                    ************************************************************

HsCD00329754        AAGCCCG-----------------------------------------------------
NM_003322.4         AA-----GCCCGGAGCTGGGCGGACGGGGAGGCCGCGGGAGGAGCCTTCCCCAGACCCAG
NM_001289395.1      AA-----GCCCG------------------------------------------------
                    **                                                          

HsCD00329754        ------------------------------------------------------------
NM_003322.4         CCCAGGCCCGGGCGCCGCAGACGGTCTACGCCAGGTTCCTCAGGGACCCCGAGGCCAAGA
NM_001289395.1      ------------------------------------------------------------
                                                                                

HsCD00329754        ---------------------------------------------------GAGAGGAGG
NM_003322.4         AGCGCGACCCCCGGGAAACCTTTCTGGTAGCCCGTGCCCCAGACGCGGAGGACGAGGAGG
NM_001289395.1      ---------------------------------------------------GAGAGGAGG
                                                                       ..*******

HsCD00329754        AGGAGGAAGAGGAGGACGAGGAGGACGAGGAAGAGGAGGCAGAGGAAAAGAAAGAGAAAA
NM_003322.4         AGGAGGAAGAGGAGGACGAGGAGGACGAGGAAGAGGAGGCAGAGGAAAAGAAAGAGAAAA
NM_001289395.1      AGGAGGAAGAGGAGGACGAGGAGGACGAGGAAGAGGAGGCAGAGGAAAAGAAAGAGAAAA
                    ************************************************************

HsCD00329754        TCCTTCTGCCTCCCAAGAAGCCCCTGAGAGAGAAGAGCTCCGCAGACCTGAAGGAGAGGA
NM_003322.4         TCCTTCTGCCTCCCAAGAAGCCCCTGAGAGAGAAGAGCTCCGCAGACCTGAAGGAGAGGA
NM_001289395.1      TCCTTCTGCCTCCCAAGAAGCCCCTGAGAGAGAAGAGCTCCGCAGACCTGAAGGAGAGGA
                    ************************************************************

HsCD00329754        GGGCCAAGGCCCAGGGCCCAAGGGGAGACCTGGGAAGCCCTGACCCCCCACCGAAACCTC
NM_003322.4         GGGCCAAGGCCCAGGGCCCAAGGGGAGACCTGGGAAGCCCTGACCCCCCACCGAAACCTC
NM_001289395.1      GGGCCAAGGCCCAGGGCCCAAGGGGAGACCTGGGAAGCCCTGACCCCCCACCGAAACCTC
                    ************************************************************

HsCD00329754        TGCGTGTTAGGAATAAGGAAGCTCCAGCAGGGGAGGGGACCAAGATGAGAAAGACCAAGA
NM_003322.4         TGCGTGTTAGGAATAAGGAAGCTCCAGCAGGGGAGGGGACCAAGATGAGAAAGACCAAGA
NM_001289395.1      TGCGTGTTAGGAATAAGGAAGCTCCAGCAGGGGAGGGGACCAAGATGAGAAAGACCAAGA
                    ************************************************************

HsCD00329754        AGAAAGGGTCTGGGGAGGCCGACAAGGACCCCTCAGGGAGCCCAGCCAGTGCGAGGAAGA
NM_003322.4         AGAAAGGGTCTGGGGAGGCCGACAAGGACCCCTCAGGGAGCCCAGCCAGTGCGAGGAAGA
NM_001289395.1      AGAAAGGGTCTGGGGAGGCCGACAAGGACCCCTCAGGGAGCCCAGCCAGTGCGAGGAAGA
                    ************************************************************

HsCD00329754        GCCCAGCAGCCATGTTTCTGGTTGGGGAAGGCAGTCCTGACAAGAAAGCCCTGAAGAAGA
NM_003322.4         GCCCAGCAGCCATGTTTCTGGTTGGGGAAGGCAGTCCTGACAAGAAAGCCCTGAAGAAGA
NM_001289395.1      GCCCAGCAGCCATGTTTCTGGTTGGGGAAGGCAGTCCTGACAAGAAAGCCCTGAAGAAGA
                    ************************************************************

HsCD00329754        AAGGCACTCCCAAAGGCGCGAGGAAGGAGGAAGAAGAGGAGGAGGAGGCAGCTACGGTGA
NM_003322.4         AAGGCACTCCCAAAGGCGCGAGGAAGGAGGAAGAAGAGGAGGAGGAGGCAGCTACGGTGA
NM_001289395.1      AAGGCACTCCCAAAGGCGCGAGGAAGGAGGAAGAAGAGGAGGAGGAGGCAGCTACGGTGA
                    ************************************************************

HsCD00329754        TAAAGAACAGCAATCAAAAGGGCAAAGCCAAAGGAAAAGGCAAAAAGAAAGCGAAGGAGG
NM_003322.4         TAAAGAAGAGCAATCAAAAGGGCAAAGCCAAAGGAAAAGGCAAAAAGAAAGCGAAGGAGG
NM_001289395.1      TAAAGAAGAGCAATCAAAAGGGCAAAGCCAAAGGAAAAGGCAAAAAGAAAGCGAAGGAGG
                    ******* ****************************************************

HsCD00329754        AGAGGGCCCCGTCTCCCCCCGTGGAGGTGGACGAACCCCGGGAGTTTGTGCTCCGGCCTG
NM_003322.4         AGAGGGCCCCGTCTCCCCCCGTGGAGGTGGACGAACCCCGGGAGTTTGTGCTCCGGCCTG
NM_001289395.1      AGAGGGCCCCGTCTCCCCCCGTGGAGGTGGACGAACCCCGGGAGTTTGTGCTCCGGCCTG
                    ************************************************************

HsCD00329754        CCCCCCAGGGCCGCACGGTGCGCTGCCGGCTGACCCGGGACAAAAAGGGCATGGATCGAG
NM_003322.4         CCCCCCAGGGCCGCACGGTGCGCTGCCGGCTGACCCGGGACAAAAAGGGCATGGATCGAG
NM_001289395.1      CCCCCCAGGGCCGCACGGTGCGCTGCCGGCTGACCCGGGACAAAAAGGGCATGGATCGAG
                    ************************************************************

HsCD00329754        GCATGTATCCCTCCTACTTCCTGCACCTGGACACGGAGAAGAAGGTGTTCCTCTTGGCTG
NM_003322.4         GCATGTATCCCTCCTACTTCCTGCACCTGGACACGGAGAAGAAGGTGTTCCTCTTGGCTG
NM_001289395.1      GCATGTATCCCTCCTACTTCCTGCACCTGGACACGGAGAAGAAGGTGTTCCTCTTGGCTG
                    ************************************************************

HsCD00329754        GCAGGAAACGAAAACGGAGCAAGACAGCCAATTACCTCATCTCCATCGACCCTACCAATC
NM_003322.4         GCAGGAAACGAAAACGGAGCAAGACAGCCAATTACCTCATCTCCATCGACCCTACCAATC
NM_001289395.1      GCAGGAAACGAAAACGGAGCAAGACAGCCAATTACCTCATCTCCATCGACCCTACCAATC
                    ************************************************************

HsCD00329754        TGTCCCGAGGAGGGGAGAATTTCATCGGGAAGCTGAGGTCCAACCTCCTGGGGAACCGCT
NM_003322.4         TGTCCCGAGGAGGGGAGAATTTCATCGGGAAGCTGAGGTCCAACCTCCTGGGGAACCGCT
NM_001289395.1      TGTCCCGAGGAGGGGAGAATTTCATCGGGAAGCTGAGGTCCAACCTCCTGGGGAACCGCT
                    ************************************************************

HsCD00329754        TCACGGTCTTTGACAACGGGCAGAACCCACAGCGTGGGTACAGCACTAATGTGGCAAGCC
NM_003322.4         TCACGGTCTTTGACAACGGGCAGAACCCACAGCGTGGGTACAGCACTAATGTGGCAAGCC
NM_001289395.1      TCACGGTCTTTGACAACGGGCAGAACCCACAGCGTGGGTACAGCACTAATGTGGCAAGCC
                    ************************************************************

HsCD00329754        TTCGGCAGGAGCTGGCAGCTGTGATCTATGAAACCAACGTGCTGGGCTTCCGTGGCCCCC
NM_003322.4         TTCGGCAGGAGCTGGCAGCTGTGATCTATGAAACCAACGTGCTGGGCTTCCGTGGCCCCC
NM_001289395.1      TTCGGCAGGAGCTGGCAGCTGTGATCTATGAAACCAACGTGCTGGGCTTCCGTGGCCCCC
                    ************************************************************

HsCD00329754        GGCGCATGACCGTCATCATTCCTGGCATGAGTGCGGAGAACGAGAGGGTCCCCATCCGGC
NM_003322.4         GGCGCATGACCGTCATCATTCCTGGCATGAGTGCGGAGAACGAGAGGGTCCCCATCCGGC
NM_001289395.1      GGCGCATGACCGTCATCATTCCTGGCATGAGTGCGGAGAACGAGAGGGTCCCCATCCGGC
                    ************************************************************

HsCD00329754        CCCGAAATGCTAGTGACGGCCTGCTGGTGCGCTGGCAGAACAAGACGCTGGAGAGCCTCA
NM_003322.4         CCCGAAATGCTAGTGACGGCCTGCTGGTGCGCTGGCAGAACAAGACGCTGGAGAGCCTCA
NM_001289395.1      CCCGAAATGCTAGTGACGGCCTGCTGGTGCGCTGGCAGAACAAGACGCTGGAGAGCCTCA
                    ************************************************************

HsCD00329754        TAGAACTGCACAACAAGCCACCTGTCTGGAACGATGACAGTGGCTCCTACACCCTCAACT
NM_003322.4         TAGAACTGCACAACAAGCCACCTGTCTGGAACGATGACAGTGGCTCCTACACCCTCAACT
NM_001289395.1      TAGAACTGCACAACAAGCCACCTGTCTGGAACGATGACAGTGGCTCCTACACCCTCAACT
                    ************************************************************

HsCD00329754        TCCAAGGCCGGGTCACCCAGGCCTCAGTCAAGAACTTCCAGATTGTCCACGCTGATGACC
NM_003322.4         TCCAAGGCCGGGTCACCCAGGCCTCAGTCAAGAACTTCCAGATTGTCCACGCTGATGACC
NM_001289395.1      TCCAAGGCCGGGTCACCCAGGCCTCAGTCAAGAACTTCCAGATTGTCCACGCTGATGACC
                    ************************************************************

HsCD00329754        CCGACTATATCGTGCTGCAGTTCGGCCGCGTGGCGGAGGACGCCTTCACCCTAGACTACC
NM_003322.4         CCGACTATATCGTGCTGCAGTTCGGCCGCGTGGCGGAGGACGCCTTCACCCTAGACTACC
NM_001289395.1      CCGACTATATCGTGCTGCAGTTCGGCCGCGTGGCGGAGGACGCCTTCACCCTAGACTACC
                    ************************************************************

HsCD00329754        GGTACCCGCTGTGCGCCCTGCAGGCCTTCGCCATCGCCCTCTCCAGTTTCGACGGGAAGC
NM_003322.4         GGTACCCGCTGTGCGCCCTGCAGGCCTTCGCCATCGCCCTCTCCAGTTTCGACGGGAAGC
NM_001289395.1      GGTACCCGCTGTGCGCCCTGCAGGCCTTCGCCATCGCCCTCTCCAGTTTCGACGGGAAGC
                    ************************************************************

HsCD00329754        TGGCCTGCGAGTTG
NM_003322.4         TGGCCTGCGAGTGA
NM_001289395.1      TGGCCTGCGAGTGA
                    ************ .

Protein

UniProt : O00294
HPRD : 03788

Clone

Cloning Information : 268495
HIP Master Clone ID : 193548
Original Clone ID : FLH268495.01L

Organism


TAX_ID : 9606
Species Specific ID: 7287

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000112041

Reagents

Labome : TULP1-antibody