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ZNF319 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF319
Gene Name:  zinc finger protein 319
Sequence             
Original Clone ID: FLH268504.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329763

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1749nts         Open reading frame : 1 to 1749
Reference Sequence Alignment

ATGTCGGAAAGCTGGCAACAGCCGCCACAGACGCAGCCGCAGCAGCCACAGCCACCGCAG
CCTCAGCACCATGCAGAGCCACCGCCAGCCCTGGCAGAGCACACGCTGCCTCCGGGCACG
GCGGAGAACCCCCTGGGCTGTGCCGTCTATGGCATCCTCCTGCAGCCAGACCCCGGCCTG
CAGCCCCCACAACACGCACCCCTGCAGGCAGCAGGAGAGCCAGGCCCCAAATGTGGCGTG
TGTGGTCACGACCTGGCGCACCTGTCCAGTCCGCATGAGCACCAGTGTCTGGCGGGCCAT
GACCGCTCATTCCAGTGCACACAGTGTCTCAAGATCTTCCACCAGGCCACTGACCTGCTG
GAGCACCAGTGCGTGCAGGCTGAGCAGAAGCCCTTCGTCTGTGGGGTCTGCAAGATGGGC
TTCTCACTACTCACCTCACTGGCACAGCACCACAGCTCCCACAGTGGCCTGGTGAAGTGT
TCCATCTGTGAAAAGACCTACAAGCCAGCTGAGGCAGCGGAGCCAGCCACCACAGCCGCC
CCGTCGCTTCCCGCAGCACCCGCGCCTTCCACTGTCACCCCTGCTGAACAGGCCGACAAG
CCCTACAGCTGCCCCATCTGCCAAAAGCCCTTCAAGCACCTGTCGGAGCTCTCTCGGCAT
GAGCGGATCCACACCGGTGAGAAGCCCTACAAGTGCACGCTGTGTGACAAAAGCTTCAGC
CAGTCGTCGCACCTGGTGCACCACAAGCGCACGCACAGCTCCGAGCGGCCCTACAAGTGC
GCAGTCTGCGAGAAGACCTTCAAGCACCGCTCTCACCTGGTGCGCCACATGTACGCGCAC
TCGGGCGAGCACCACCTGTTCCGCTGCAACGTGTGCGAGTTGCATTTCAAGGAGTCGTCG
GAGCTGCTGCAGCACCCGTGCACGCCAAGTGGGGAGCGGCCCTTCCGCTGCGGCGAGTGC
CAGAAGGCCTTCAAGCGGCCCTCGGACCTGCGGCAGCATGAGCGCACACACAGCGCCGAG
CGGCCCTTCAAGTGCGACCTGTGCCCCATGGGCTTCAAGCAGCAGTACGCACTGATGCGC
CACCGGCGCACACACAAGACCGAGGAGCCCTTCAAATGCGGCCTATGTGAGAAGGGCTTT
GGGCAGCCCAGCCACCTGCTCTACCACCAGCACGTGCACACCCTCGAGACTCTCTTCAAG
TGCCCCGTGTGCCAGAAAGGCTTTGACCAATCTGCCGAGCTGCTGCGGCACAAGTGCCTG
CCCGGCGCGGCCGAGCGGCCCTTCAAGTGCCCTGTGTGCAACAAGGCCTACAAGCGCGCG
TCGGCCCTGCAGAAGCACCAGCTGGCCCACTGTGCGGCGGCAGAGAAGCCCCTGCGCTGC
ACGCTTTGCGAACGCCGCTTCTTCTCCTCTTCCGAGTTCGTGCAGCACCGCTGCGATCCG
GCCCGCGAGAAGCCACTCAAGTGCCCAGACTGCGAGAAACGCTTCAAGTACGCGTCAGAC
CTGCAGCGGCACCGGCGGGTACACACAGGCGAGAAACCCTACAAGTGCCCCAACTGCGAC
AAGGCCTTCAAGCAGCGGGAGCATCTCAACAAGCACCAGGGCGTGCACGCCCGCGAGCAG
CAGTTCAAGTGTGTATGGTGCGGGGAGCGCTTCCTAGACGTGGCCTTGTTGCAGGAGCAC
AGCGCGCAGCACAGTGCCGCCGCCGCGGCAGCGGAGGGCGCCTACCAGGTAGCCGCCTGC
CTGCCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1811
Start on reference sequence 63
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF319
Symbol Nomenclature : ZNF319
SYNONYM : ZFP319
Full Nomenclature : zinc finger protein 319
GENEID : 57567
HGNC : 13644
Vega : OTTHUMG00000175512
Target GenBank: BC101767

 

Reference Sequence Alignment

  

HsCD00329763        ATGTCGGAAAGCTGGCAACAGCCGCCACAGACGCAGCCGCAGCAGCCACAGCCACCGCAG
XM_005256069.1      ATGTCGGAAAGCTGGCAACAGCCGCCACAGACGCAGCCGCAGCAGCCACAGCCACCGCAG
NM_020807.1         ATGTCGGAAAGCTGGCAACAGCCGCCACAGACGCAGCCGCAGCAGCCACAGCCACCGCAG
                    ************************************************************

HsCD00329763        CCTCAGCACCATGCAGAGCCACCGCCAGCCCTGGCAGAGCACACGCTGCCTCCGGGCACG
XM_005256069.1      CCTCAGCACCATGCAGAGCCACCGCCAGCCCTGGCAGAGCACACGCTGCCTCCGGGCACG
NM_020807.1         CCTCAGCACCATGCAGAGCCACCGCCAGCCCTGGCAGAGCACACGCTGCCTCCGGGCACG
                    ************************************************************

HsCD00329763        GCGGAGAACCCCCTGGGCTGTGCCGTCTATGGCATCCTCCTGCAGCCAGACCCCGGCCTG
XM_005256069.1      GCGGAGAACCCCCTGGGCTGTGCCGTCTATGGCATCCTCCTGCAGCCAGACCCCGGCCTG
NM_020807.1         GCGGAGAACCCCCTGGGCTGTGCTGTCTATGGCATCCTCCTGCAGCCAGACCCCGGCCTG
                    *********************** ************************************

HsCD00329763        CAGCCCCCACAACACGCACCCCTGCAGGCAGCAGGAGAGCCAGGCCCCAAATGTGGCGTG
XM_005256069.1      CAGCCCCCACAACACGCACCCCTGCAGGCAGCAGGAGAGCCAGGCCCCAAATGTGGCGTG
NM_020807.1         CAGCCCCCACAACACGCACCCCTGCAGGCAGCAGGAGAGCCAGGCCCCAAATGTGGCGTG
                    ************************************************************

HsCD00329763        TGTGGTCACGACCTGGCGCACCTGTCCAGTCCGCATGAGCACCAGTGTCTGGCGGGCCAT
XM_005256069.1      TGTGGTCACGACCTGGCGCACCTGTCCAGTCCGCATGAGCACCAGTGTCTGGCGGGCCAT
NM_020807.1         TGTGGTCACGACCTGGCGCACCTGTCCAGTCCGCATGAGCACCAGTGTCTGGCGGGCCAT
                    ************************************************************

HsCD00329763        GACCGCTCATTCCAGTGCACACAGTGTCTCAAGATCTTCCACCAGGCCACTGACCTGCTG
XM_005256069.1      GACCGCTCATTCCAGTGCACACAGTGTCTCAAGATCTTCCACCAGGCCACTGACCTGCTG
NM_020807.1         GACCGCTCATTCCAGTGCACACAGTGTCTCAAGATCTTCCACCAGGCCACTGACCTGCTG
                    ************************************************************

HsCD00329763        GAGCACCAGTGCGTGCAGGCTGAGCAGAAGCCCTTCGTCTGTGGGGTCTGCAAGATGGGC
XM_005256069.1      GAGCACCAGTGCGTGCAGGCTGAGCAGAAGCCCTTCGTCTGTGGGGTCTGCAAGATGGGC
NM_020807.1         GAGCACCAGTGCGTGCAGGCTGAGCAGAAGCCCTTCGTCTGTGGGGTCTGCAAGATGGGC
                    ************************************************************

HsCD00329763        TTCTCACTACTCACCTCACTGGCACAGCACCACAGCTCCCACAGTGGCCTGGTGAAGTGT
XM_005256069.1      TTCTCACTACTCACCTCACTGGCACAGCACCACAGCTCCCACAGTGGCCTGGTGAAGTGT
NM_020807.1         TTCTCACTACTCACCTCACTGGCACAGCACCACAGCTCCCACAGTGGCCTGGTGAAGTGT
                    ************************************************************

HsCD00329763        TCCATCTGTGAAAAGACCTACAAGCCAGCTGAGGCAGCGGAGCCAGCCACCACAGCCGCC
XM_005256069.1      TCCATCTGTGAAAAGACCTACAAGCCAGCTGAGGCAGCGGAGCCAGCCACCACAGCCGCC
NM_020807.1         TCCATCTGTGAAAAGACCTACAAGCCAGCTGAGGCAGCGGAGCCAGCCACCACAGCCGCC
                    ************************************************************

HsCD00329763        CCGTCGCTTCCCGCAGCACCCGCGCCTTCCACTGTCACCCCTGCTGAACAGGCCGACAAG
XM_005256069.1      CCGTCGCTTCCCGCAGCACCCGCGCCTTCCACTGTCACCCCTGCTGAACAGGCCGACAAG
NM_020807.1         CCGTCGCTTCCCGCAGCACCCGCGCCTTCCACTGTCACCCCTGCTGAACAGGCCGACAAG
                    ************************************************************

HsCD00329763        CCCTACAGCTGCCCCATCTGCCAAAAGCCCTTCAAGCACCTGTCGGAGCTCTCTCGGCAT
XM_005256069.1      CCCTACAGCTGCCCCATCTGCCAAAAGCCCTTCAAGCACCTGTCGGAGCTCTCTCGGCAT
NM_020807.1         CCCTACAGCTGCCCCATCTGCCAAAAGCCCTTCAAGCACCTGTCGGAGCTCTCTCGGCAT
                    ************************************************************

HsCD00329763        GAGCGGATCCACACCGGTGAGAAGCCCTACAAGTGCACGCTGTGTGACAAAAGCTTCAGC
XM_005256069.1      GAGCGGATCCACACCGGTGAGAAGCCCTACAAGTGCACGCTGTGTGACAAAAGCTTCAGC
NM_020807.1         GAGCGGATCCACACCGGTGAGAAGCCCTACAAGTGCACGCTGTGTGACAAAAGCTTCAGC
                    ************************************************************

HsCD00329763        CAGTCGTCGCACCTGGTGCACCACAAGCGCACGCACAGCTCCGAGCGGCCCTACAAGTGC
XM_005256069.1      CAGTCGTCGCACCTGGTGCACCACAAGCGCACGCACAGCTCCGAGCGGCCCTACAAGTGC
NM_020807.1         CAGTCGTCGCACCTGGTGCACCACAAGCGCACGCACAGCTCCGAGCGGCCCTACAAGTGC
                    ************************************************************

HsCD00329763        GCAGTCTGCGAGAAGACCTTCAAGCACCGCTCTCACCTGGTGCGCCACATGTACGCGCAC
XM_005256069.1      GCAGTCTGCGAGAAGACCTTCAAGCACCGCTCTCACCTGGTGCGCCACATGTACGCGCAC
NM_020807.1         GCAGTCTGCGAGAAGACCTTCAAGCACCGCTCTCACCTGGTGCGCCACATGTACGCGCAC
                    ************************************************************

HsCD00329763        TCGGGCGAGCACCACCTGTTCCGCTGCAACGTGTGCGAGTTGCATTTCAAGGAGTCGTCG
XM_005256069.1      TCGGGCGAGCACCACCTGTTCCGCTGCAACGTGTGCGAGTTGCATTTCAAGGAGTCGTCG
NM_020807.1         TCGGGCGAGCACCACCTGTTCCGCTGCAACGTGTGCGAGTTGCATTTCAAGGAGTCGTCG
                    ************************************************************

HsCD00329763        GAGCTGCTGCAGCACCCGTGCACGCCAAGTGGGGAGCGGCCCTTCCGCTGCGGCGAGTGC
XM_005256069.1      GAGCTGCTGCAGCACCCGTGCACGCCAAGTGGGGAGCGGCCCTTCCGCTGCGGCGAGTGC
NM_020807.1         GAGCTGCTGCAGCACCCGTGCACGCCAAGTGGGGAGCGGCCCTTCCGCTGCGGCGAGTGC
                    ************************************************************

HsCD00329763        CAGAAGGCCTTCAAGCGGCCCTCGGACCTGCGGCAGCATGAGCGCACACACAGCGCCGAG
XM_005256069.1      CAGAAGGCCTTCAAGCGGCCCTCGGACCTGCGGCAGCATGAGCGCACACACAGCGCCGAG
NM_020807.1         CAGAAGGCCTTCAAGCGGCCCTCGGACCTGCGGCAGCATGAGCGCACACACAGCGCCGAG
                    ************************************************************

HsCD00329763        CGGCCCTTCAAGTGCGACCTGTGCCCCATGGGCTTCAAGCAGCAGTACGCACTGATGCGC
XM_005256069.1      CGGCCCTTCAAGTGCGACCTGTGCCCCATGGGCTTCAAGCAGCAGTACGCACTGATGCGC
NM_020807.1         CGGCCCTTCAAGTGCGACCTGTGCCCCATGGGCTTCAAGCAGCAGTACGCACTGATGCGC
                    ************************************************************

HsCD00329763        CACCGGCGCACACACAAGACCGAGGAGCCCTTCAAATGCGGCCTATGTGAGAAGGGCTTT
XM_005256069.1      CACCGGCGCACACACAAGACCGAGGAGCCCTTCAAATGCGGCCTATGTGAGAAGGGCTTT
NM_020807.1         CACCGGCGCACACACAAGACCGAGGAGCCCTTCAAATGCGGCCTATGTGAGAAGGGCTTT
                    ************************************************************

HsCD00329763        GGGCAGCCCAGCCACCTGCTCTACCACCAGCACGTGCACACCCTCGAGACTCTCTTCAAG
XM_005256069.1      GGGCAGCCCAGCCACCTGCTCTACCACCAGCACGTGCACACCCTCGAGACTCTCTTCAAG
NM_020807.1         GGGCAGCCCAGCCACCTGCTCTACCACCAGCACGTGCACACCCTCGAGACTCTCTTCAAG
                    ************************************************************

HsCD00329763        TGCCCCGTGTGCCAGAAAGGCTTTGACCAATCTGCCGAGCTGCTGCGGCACAAGTGCCTG
XM_005256069.1      TGCCCCGTGTGCCAGAAAGGCTTTGACCAATCTGCCGAGCTGCTGCGGCACAAGTGCCTG
NM_020807.1         TGCCCCGTGTGCCAGAAAGGCTTTGACCAATCTGCCGAGCTGCTGCGGCACAAGTGCCTG
                    ************************************************************

HsCD00329763        CCCGGCGCGGCCGAGCGGCCCTTCAAGTGCCCTGTGTGCAACAAGGCCTACAAGCGCGCG
XM_005256069.1      CCCGGCGCGGCCGAGCGGCCCTTCAAGTGCCCTGTGTGCAACAAGGCCTACAAGCGCGCG
NM_020807.1         CCCGGCGCGGCCGAGCGGCCCTTCAAGTGCCCTGTGTGCAACAAGGCCTACAAGCGCGCG
                    ************************************************************

HsCD00329763        TCGGCCCTGCAGAAGCACCAGCTGGCCCACTGTGCGGCGGCAGAGAAGCCCCTGCGCTGC
XM_005256069.1      TCGGCCCTGCAGAAGCACCAGCTGGCCCACTGTGCGGCGGCAGAGAAGCCCCTGCGCTGC
NM_020807.1         TCGGCCCTGCAGAAGCACCAGCTGGCCCACTGTGCGGCGGCAGAGAAGCCCCTGCGCTGC
                    ************************************************************

HsCD00329763        ACGCTTTGCGAACGCCGCTTCTTCTCCTCTTCCGAGTTCGTGCAGCACCGCTGCGATCCG
XM_005256069.1      ACGCTTTGCGAACGCCGCTTCTTCTCCTCTTCCGAGTTCGTGCAGCACCGCTGCGATCCG
NM_020807.1         ACGCTTTGCGAACGCCGCTTCTTCTCCTCTTCCGAGTTCGTGCAGCACCGCTGCGATCCG
                    ************************************************************

HsCD00329763        GCCCGCGAGAAGCCACTCAAGTGCCCAGACTGCGAGAAACGCTTCAAGTACGCGTCAGAC
XM_005256069.1      GCCCGCGAGAAGCCACTCAAGTGCCCAGACTGCGAGAAACGCTTCAAGTACGCGTCAGAC
NM_020807.1         GCCCGCGAGAAGCCACTCAAGTGCCCAGACTGCGAGAAACGCTTCAAGTACGCGTCAGAC
                    ************************************************************

HsCD00329763        CTGCAGCGGCACCGGCGGGTACACACAGGCGAGAAACCCTACAAGTGCCCCAACTGCGAC
XM_005256069.1      CTGCAGCGGCACCGGCGGGTACACACAGGCGAGAAGCCCTACAAGTGCCCCAACTGCGAC
NM_020807.1         CTGCAGCGGCACCGGCGGGTACACACAGGCGAGAAACCCTACAAGTGCCCCAACTGCGAC
                    ***********************************.************************

HsCD00329763        AAGGCCTTCAAGCAGCGGGAGCATCTCAACAAGCACCAGGGCGTGCACGCCCGCGAGCAG
XM_005256069.1      AAGGCCTTCAAGCAGCGGGAGCATCTCAACAAGCACCAGGGCGTGCACGCCCGCGAGCAG
NM_020807.1         AAGGCCTTCAAGCAGCGGGAGCATCTCAACAAGCACCAGGGCGTGCACGCCCGCGAGCAG
                    ************************************************************

HsCD00329763        CAGTTCAAGTGTGTATGGTGCGGGGAGCGCTTCCTAGACGTGGCCTTGTTGCAGGAGCAC
XM_005256069.1      CAGTTCAAGTGTGTATGGTGCGGGGAGCGCTTCCTAGACGTGGCCTTGTTGCAGGAGCAC
NM_020807.1         CAGTTCAAGTGTGTATGGTGCGGGGAGCGCTTCCTAGACGTGGCCTTGTTGCAGGAGCAC
                    ************************************************************

HsCD00329763        AGCGCGCAGCACAGTGCCGCCGCCGCGGCAGCGGAGGGCGCCTACCAGGTAGCCGCCTGC
XM_005256069.1      AGCGCGCAGCACAGTGCCGCCGCCGCGGCAGCGGAGGGCGCCTACCAGGTAGCCGCCTGC
NM_020807.1         AGCGCGCAGCACAGTGCCGCCGCCGCGGCAGCGGAGGGCGCCTACCAGGTAGCCGCCTGC
                    ************************************************************

HsCD00329763        CTGCCCTTG
XM_005256069.1      CTGCCCTGA
NM_020807.1         CTGCCCTGA
                    ******* .

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9P2F9
HPRD : 18326

Clone

Cloning Information : 268504
HIP Master Clone ID : 193569
Original Clone ID : FLH268504.01L

Organism


TAX_ID : 9606
Species Specific ID: 57567

Genome

Chromosome : 16
Map Location : 16q21
Ensembl : ENSG00000166188

Reagents

Labome : ZNF319-antibody