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ZNF436 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF436
Gene Name:  zinc finger protein 436
Sequence             
Original Clone ID: FLH268523.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329782

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1413nts         Open reading frame : 1 to 1413
Reference Sequence Alignment

ATGGCAGCCACCCTGCTCATGGCTGGGTCCCAGGCACCTGTGACGTTTGAAGATATGGCC
ATGTATCTCACCCGGGAAGAATGGAGACCTCTGGACGCTGCACAGAGGGACCTTTACCGG
GATGTTATGCAGGAGAATTATGGAAATGTTGTCTCACTAGATTTTGAGATCAGGAGTGAG
AACGAGGTAAATCCCAAGCAAGAGATTAGTGAAGATGTACAATTTGGGACTACATCTGAA
AGACCTGCTGAGAATGCTGAGGAAAATCCTGAAAGTGAAGAGGGCTTTGAAAGCGGAGAT
AGGTCAGAAAGACAATGGGGAGATTTAACAGCAGAAGAGTGGGTAAGCTATCCTCTCCAA
CCAGTCACTGATCTACTTGTCCACAAAGAAGTCCACACAGGCATCCGCTATCATATATGT
TCTCATTGTGGAAAGGCCTTCAGTCAGATCTCAGACCTTAATCGACATCAGAAGACCCAC
ACTGGAGACAGACCCTATAAATGTTATGAATGTGGAAAAGGCTTCAGTCGCAGCTCACAC
CTTATTCAGCATCAAAGAACACATACTGGGGAGAGGCCTTATGACTGTAACGAGTGTGGG
AAAAGTTTTGGAAGAAGTTCTCACCTGATTCAGCATCAGACAATCCACACTGGAGAGAAG
CCTCACAAATGTAATGAGTGTGGAAAAAGTTTCTGCCGTCTCTCTCACCTAATCCAACAC
CAAAGGACCCACAGTGGTGAGAAACCCTATGAGTGTGAGGAGTGTGGGAAAAGCTTCAGC
CGGAGCTCTCACCTAGCTCAGCACCAGAGGACCCACACGGGTGAGAAACCTTATGAATGT
AACGAATGTGGCCGAGGCTTCAGTGAGAGATCTGATCTCATCAAACACTATCGAGTCCAC
ACAGGGGAGAGGCCCTACAAGTGTGATGAGTGTGGGAAGAACTTCAGTCAGAACTCCGAC
CTTGTGCGTCATCGCAGAGCCCACACGGGAGAGAAGCCATACCACTGTAACGAATGTGGG
GAAAATTTCAGCCGCATCTCACACTTGGTTCAGCACCAGAGAACTCACACTGGAGAGAAG
CCATATGAATGCAATGCTTGTGGGAAAAGCTTCAGCCGGAGCTCTCATCTCATCACACAC
CAGAAAATTCACACTGGAGAGAAGCCTTATGAGTGTAATGAGTGTTGGCGAAGCTTTGGT
GAAAGGTCAGATCTAATTAAACATCAGAGAACCCACACAGGGGAGAAGCCCTACGAGTGT
GTGCAGTGTGGGAAAGGTTTCACCCAGAGCTCCAACCTCATCACACATCAAAGAGTTCAC
ACGGGAGAGAAACCTTATGAATGTACCGAATGTGAGAAGAGTTTCAGCAGGAGCTCAGCT
CTTATTAAACATAAGAGAGTTCATACGGACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1705
Start on reference sequence 293
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF436
Symbol Nomenclature : ZNF436
SYNONYM : ZNF; Zfp46
Designation : DNA-binding protein
Full Nomenclature : zinc finger protein 436
GENEID : 80818
Locus Tag : RP5-1057J7.3
HGNC : 20814
MIM : 611703
Vega : OTTHUMG00000003232
Target GenBank: BC056400

 

Reference Sequence Alignment

  

HsCD00329782        ATGGCAGCCACCCTGCTCATGGCTGGGTCCCAGGCACCTGTGACGTTTGAAGATATGGCC
NM_001077195.1      ATGGCAGCCACCCTGCTCATGGCTGGGTCCCAGGCACCTGTGACGTTTGAAGATATGGCC
NM_030634.2         ATGGCAGCCACCCTGCTCATGGCTGGGTCCCAGGCACCTGTGACGTTTGAAGATATGGCC
                    ************************************************************

HsCD00329782        ATGTATCTCACCCGGGAAGAATGGAGACCTCTGGACGCTGCACAGAGGGACCTTTACCGG
NM_001077195.1      ATGTATCTCACCCGGGAAGAATGGAGACCTCTGGACGCTGCACAGAGGGACCTTTACCGG
NM_030634.2         ATGTATCTCACCCGGGAAGAATGGAGACCTCTGGACGCTGCACAGAGGGACCTTTACCGG
                    ************************************************************

HsCD00329782        GATGTTATGCAGGAGAATTATGGAAATGTTGTCTCACTAGATTTTGAGATCAGGAGTGAG
NM_001077195.1      GATGTTATGCAGGAGAATTATGGAAATGTTGTCTCACTAGATTTTGAGATCAGGAGTGAG
NM_030634.2         GATGTTATGCAGGAGAATTATGGAAATGTTGTCTCACTAGATTTTGAGATCAGGAGTGAG
                    ************************************************************

HsCD00329782        AACGAGGTAAATCCCAAGCAAGAGATTAGTGAAGATGTACAATTTGGGACTACATCTGAA
NM_001077195.1      AACGAGGTAAATCCCAAGCAAGAGATTAGTGAAGATGTACAATTTGGGACTACATCTGAA
NM_030634.2         AACGAGGTAAATCCCAAGCAAGAGATTAGTGAAGATGTACAATTTGGGACTACATCTGAA
                    ************************************************************

HsCD00329782        AGACCTGCTGAGAATGCTGAGGAAAATCCTGAAAGTGAAGAGGGCTTTGAAAGCGGAGAT
NM_001077195.1      AGACCTGCTGAGAATGCTGAGGAAAATCCTGAAAGTGAAGAGGGCTTTGAAAGCGGAGAT
NM_030634.2         AGACCTGCTGAGAATGCTGAGGAAAATCCTGAAAGTGAAGAGGGCTTTGAAAGCGGAGAT
                    ************************************************************

HsCD00329782        AGGTCAGAAAGACAATGGGGAGATTTAACAGCAGAAGAGTGGGTAAGCTATCCTCTCCAA
NM_001077195.1      AGGTCAGAAAGACAATGGGGAGATTTAACAGCAGAAGAGTGGGTAAGCTATCCTCTCCAA
NM_030634.2         AGGTCAGAAAGACAATGGGGAGATTTAACAGCAGAAGAGTGGGTAAGCTATCCTCTCCAA
                    ************************************************************

HsCD00329782        CCAGTCACTGATCTACTTGTCCACAAAGAAGTCCACACAGGCATCCGCTATCATATATGT
NM_001077195.1      CCAGTCACTGATCTACTTGTCCACAAAGAAGTCCACACAGGCATCCGCTATCATATATGT
NM_030634.2         CCAGTCACTGATCTACTTGTCCACAAAGAAGTCCACACAGGCATCCGCTATCATATATGT
                    ************************************************************

HsCD00329782        TCTCATTGTGGAAAGGCCTTCAGTCAGATCTCAGACCTTAATCGACATCAGAAGACCCAC
NM_001077195.1      TCTCATTGTGGAAAGGCCTTCAGTCAGATCTCAGACCTTAATCGACATCAGAAGACCCAC
NM_030634.2         TCTCATTGTGGAAAGGCCTTCAGTCAGATCTCAGACCTTAATCGACATCAGAAGACCCAC
                    ************************************************************

HsCD00329782        ACTGGAGACAGACCCTATAAATGTTATGAATGTGGAAAAGGCTTCAGTCGCAGCTCACAC
NM_001077195.1      ACTGGAGACAGACCCTATAAATGTTATGAATGTGGAAAAGGCTTCAGTCGCAGCTCACAC
NM_030634.2         ACTGGAGACAGACCCTATAAATGTTATGAATGTGGAAAAGGCTTCAGTCGCAGCTCACAC
                    ************************************************************

HsCD00329782        CTTATTCAGCATCAAAGAACACATACTGGGGAGAGGCCTTATGACTGTAACGAGTGTGGG
NM_001077195.1      CTTATTCAGCATCAAAGAACACATACTGGGGAGAGGCCTTATGACTGTAACGAGTGTGGG
NM_030634.2         CTTATTCAGCATCAAAGAACACATACTGGGGAGAGGCCTTATGACTGTAACGAGTGTGGG
                    ************************************************************

HsCD00329782        AAAAGTTTTGGAAGAAGTTCTCACCTGATTCAGCATCAGACAATCCACACTGGAGAGAAG
NM_001077195.1      AAAAGTTTTGGAAGAAGTTCTCACCTGATTCAGCATCAGACAATCCACACTGGAGAGAAG
NM_030634.2         AAAAGTTTTGGAAGAAGTTCTCACCTGATTCAGCATCAGACAATCCACACTGGAGAGAAG
                    ************************************************************

HsCD00329782        CCTCACAAATGTAATGAGTGTGGAAAAAGTTTCTGCCGTCTCTCTCACCTAATCCAACAC
NM_001077195.1      CCTCACAAATGTAATGAGTGTGGAAAAAGTTTCTGCCGTCTCTCTCACCTAATCCAACAC
NM_030634.2         CCTCACAAATGTAATGAGTGTGGAAAAAGTTTCTGCCGTCTCTCTCACCTAATCCAACAC
                    ************************************************************

HsCD00329782        CAAAGGACCCACAGTGGTGAGAAACCCTATGAGTGTGAGGAGTGTGGGAAAAGCTTCAGC
NM_001077195.1      CAAAGGACCCACAGTGGTGAGAAACCCTATGAGTGTGAGGAGTGTGGGAAAAGCTTCAGC
NM_030634.2         CAAAGGACCCACAGTGGTGAGAAACCCTATGAGTGTGAGGAGTGTGGGAAAAGCTTCAGC
                    ************************************************************

HsCD00329782        CGGAGCTCTCACCTAGCTCAGCACCAGAGGACCCACACGGGTGAGAAACCTTATGAATGT
NM_001077195.1      CGGAGCTCTCACCTAGCTCAGCACCAGAGGACCCACACGGGTGAGAAACCTTATGAATGT
NM_030634.2         CGGAGCTCTCACCTAGCTCAGCACCAGAGGACCCACACGGGTGAGAAACCTTATGAATGT
                    ************************************************************

HsCD00329782        AACGAATGTGGCCGAGGCTTCAGTGAGAGATCTGATCTCATCAAACACTATCGAGTCCAC
NM_001077195.1      AACGAATGTGGCCGAGGCTTCAGTGAGAGATCTGATCTCATCAAACACTATCGAGTCCAC
NM_030634.2         AACGAATGTGGCCGAGGCTTCAGTGAGAGATCTGATCTCATCAAACACTATCGAGTCCAC
                    ************************************************************

HsCD00329782        ACAGGGGAGAGGCCCTACAAGTGTGATGAGTGTGGGAAGAACTTCAGTCAGAACTCCGAC
NM_001077195.1      ACAGGGGAGAGGCCCTACAAGTGTGATGAGTGTGGGAAGAATTTCAGTCAGAACTCCGAC
NM_030634.2         ACAGGGGAGAGGCCCTACAAGTGTGATGAGTGTGGGAAGAATTTCAGTCAGAACTCCGAC
                    ***************************************** ******************

HsCD00329782        CTTGTGCGTCATCGCAGAGCCCACACGGGAGAGAAGCCATACCACTGTAACGAATGTGGG
NM_001077195.1      CTTGTGCGTCATCGCAGAGCCCACACGGGAGAGAAGCCATACCACTGTAACGAATGTGGG
NM_030634.2         CTTGTGCGTCATCGCAGAGCCCACACGGGAGAGAAGCCATACCACTGTAACGAATGTGGG
                    ************************************************************

HsCD00329782        GAAAATTTCAGCCGCATCTCACACTTGGTTCAGCACCAGAGAACTCACACTGGAGAGAAG
NM_001077195.1      GAAAATTTCAGCCGCATCTCACACTTGGTTCAGCACCAGAGAACTCACACTGGAGAGAAG
NM_030634.2         GAAAATTTCAGCCGCATCTCACACTTGGTTCAGCACCAGAGAACTCACACTGGAGAGAAG
                    ************************************************************

HsCD00329782        CCATATGAATGCAATGCTTGTGGGAAAAGCTTCAGCCGGAGCTCTCATCTCATCACACAC
NM_001077195.1      CCATATGAATGCAATGCTTGTGGGAAAAGCTTCAGCCGGAGCTCTCATCTCATCACACAC
NM_030634.2         CCATATGAATGCAATGCTTGTGGGAAAAGCTTCAGCCGGAGCTCTCATCTCATCACACAC
                    ************************************************************

HsCD00329782        CAGAAAATTCACACTGGAGAGAAGCCTTATGAGTGTAATGAGTGTTGGCGAAGCTTTGGT
NM_001077195.1      CAGAAAATTCACACTGGAGAGAAGCCTTATGAGTGTAATGAGTGTTGGCGAAGCTTTGGT
NM_030634.2         CAGAAAATTCACACTGGAGAGAAGCCTTATGAGTGTAATGAGTGTTGGCGAAGCTTTGGT
                    ************************************************************

HsCD00329782        GAAAGGTCAGATCTAATTAAACATCAGAGAACCCACACAGGGGAGAAGCCCTACGAGTGT
NM_001077195.1      GAAAGGTCAGATCTAATTAAACATCAGAGAACCCACACAGGGGAGAAGCCCTACGAGTGT
NM_030634.2         GAAAGGTCAGATCTAATTAAACATCAGAGAACCCACACAGGGGAGAAGCCCTACGAGTGT
                    ************************************************************

HsCD00329782        GTGCAGTGTGGGAAAGGTTTCACCCAGAGCTCCAACCTCATCACACATCAAAGAGTTCAC
NM_001077195.1      GTGCAGTGTGGGAAAGGTTTCACCCAGAGCTCCAACCTCATCACACATCAAAGAGTTCAC
NM_030634.2         GTGCAGTGTGGGAAAGGTTTCACCCAGAGCTCCAACCTCATCACACATCAAAGAGTTCAC
                    ************************************************************

HsCD00329782        ACGGGAGAGAAACCTTATGAATGTACCGAATGTGAGAAGAGTTTCAGCAGGAGCTCAGCT
NM_001077195.1      ACGGGAGAGAAACCTTATGAATGTACCGAATGTGAGAAGAGTTTCAGCAGGAGCTCAGCT
NM_030634.2         ACGGGAGAGAAACCTTATGAATGTACCGAATGTGAGAAGAGTTTCAGCAGGAGCTCAGCT
                    ************************************************************

HsCD00329782        CTTATTAAACATAAGAGAGTTCATACGGACTTG
NM_001077195.1      CTTATTAAACATAAGAGAGTTCATACGGACTAA
NM_030634.2         CTTATTAAACATAAGAGAGTTCATACGGACTAA
                    *******************************:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9C0F3
HPRD : 15793

Clone

Cloning Information : 268523
HIP Master Clone ID : 194763
Original Clone ID : FLH268523.01L

Organism


TAX_ID : 9606
Species Specific ID: 80818

Genome

Chromosome : 1
Map Location : 1p36
Ensembl : ENSG00000125945

Reagents

Labome : ZNF436-antibody