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ILF2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ILF2
Gene Name:  interleukin enhancer binding factor 2
Sequence             
Original Clone ID: FLH269317.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329821

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1173nts         Open reading frame : 1 to 1173
Reference Sequence Alignment

ATGAGGGGTGACAGAGGCCGTGGTCGTGGTGGGCGCTTTGGTTCCAGAGGAGGCCCAGGA
GGAGGGTTCAGGCCCTTTGTACCACATATCCCATTTGACTTCTATTTGTGTGAAATGGCC
TTTCCCCGGGTCAAGCCAGCACCTGATGAAACTTCCTTCAGTGAGGCCTTGCTGAAGAGG
AATCAGGACCTGGCTCCCAATTCTGCTGAACAGGCATCTATCCTTTCTCTGGTGACAAAA
ATAAACAATGTGATTGATAATCTGATTGTGGCTCCAGGGACATTTGAAGTGCAAATTGAA
GAAGTTCGACAGGTGGGATCCTATAAAAAGGGGACAATGACTACAGGACACAATGTGGCT
GACCTGGTGGTGATACTCAAGATTCTGCCAACGTTGGAAGCTGTTGCTGCCCTGGGGAAC
AAAGTCGTGGAAAGCCTAAGAGCACAGGATCCTTCTGAAGTTTTAACCATGCTGACCAAC
GAAACTGGCTTTGAAATCAGTTCTTCTGATGCTACAGTGAAGATTCTCATTACAACAGTG
CCACCCAATCTTCGAAAACTGGATCCAGAACTCCATTTGGATATCAAAGTATTGCAGAGT
GCCTTAGCAGCCATCCGACATGCCCGCTGGTTCGAGGAAAATGCTTCTCAGTCCACAGTT
AAAGTTCTCATCAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGCCCCTC
ACACCCTGGATCCTTGACCTACTAGGCCATTATGCTGTGATGAACAACCCCACCAGACAG
CCTTTGGCCCTAAACGTTGCATACAGGCGCTGCTTGCAGATTCTGGCTGCAGGACTGTTC
CTGCCAGGTTCAGTGGGTATCACTGACCCCTGTGAGAGTGGCAACTTTAGAGTACACACA
GTCATGACCCTAGAACAGCAGGACATGGTCTGCTATACAGCTCAGACTCTCGTCCGAATC
CTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGGTGATGCCAGCTATCTTGCT
TCTGAAATATCTACCTGGGATGGAGTGATAGTAACACCTTCAGAAAAGGCTTATGAGAAG
CCACCAGAGAAGAAGGAAGGAGAGGAAGAAGAGGAGAATACAGAAGAACCACCTCAAGGA
GAGGAAGAAGAAAGCATGGAAACTCAGGAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1213
Start on reference sequence 41
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ILF2
Symbol Nomenclature : ILF2
SYNONYM : NF45; PRO3063
Designation : interleukin enhancer binding factor 2, 45kD|interleukin enhancer binding factor 2, 45kDa|interleukin enhancer-binding factor 2|nuclear factor of activated T-cells, 45-kDa
Full Nomenclature : interleukin enhancer binding factor 2
GENEID : 3608
Locus Tag : RP11-354A16.1
HGNC : 6037
MIM : 603181
Vega : OTTHUMG00000037087
Target GenBank: BC000382

 

Reference Sequence Alignment

  

HsCD00329821        ATGAGGGGTGACAGAGGCCGTGGTCGTGGTGGGCGCTTTGGTTCCAGAGGAGGCCCAGGA
NM_004515.3         ATGAGGGGTGACAGAGGCCGTGGTCGTGGTGGGCGCTTTGGTTCCAGAGGAGGCCCAGGA
NM_001267809.1      ------------------------------------------------------------
                                                                                

HsCD00329821        GGAGGGTTCAGGCCCTTTGTACCACATATCCCATTTGACTTCTATTTGTGTGAAATGGCC
NM_004515.3         GGAGGGTTCAGGCCCTTTGTACCACATATCCCATTTGACTTCTATTTGTGTGAAATGGCC
NM_001267809.1      ------------------------------------------------------ATGGCC
                                                                          ******

HsCD00329821        TTTCCCCGGGTCAAGCCAGCACCTGATGAAACTTCCTTCAGTGAGGCCTTGCTGAAGAGG
NM_004515.3         TTTCCCCGGGTCAAGCCAGCACCTGATGAAACTTCCTTCAGTGAGGCCTTGCTGAAGAGG
NM_001267809.1      TTTCCCCGGGTCAAGCCAGCACCTGATGAAACTTCCTTCAGTGAGGCCTTGCTGAAGAGG
                    ************************************************************

HsCD00329821        AATCAGGACCTGGCTCCCAATTCTGCTGAACAGGCATCTATCCTTTCTCTGGTGACAAAA
NM_004515.3         AATCAGGACCTGGCTCCCAATTCTGCTGAACAGGCATCTATCCTTTCTCTGGTGACAAAA
NM_001267809.1      AATCAGGACCTGGCTCCCAATTCTGCTGAACAGGCATCTATCCTTTCTCTGGTGACAAAA
                    ************************************************************

HsCD00329821        ATAAACAATGTGATTGATAATCTGATTGTGGCTCCAGGGACATTTGAAGTGCAAATTGAA
NM_004515.3         ATAAACAATGTGATTGATAATCTGATTGTGGCTCCAGGGACATTTGAAGTGCAAATTGAA
NM_001267809.1      ATAAACAATGTGATTGATAATCTGATTGTGGCTCCAGGGACATTTGAAGTGCAAATTGAA
                    ************************************************************

HsCD00329821        GAAGTTCGACAGGTGGGATCCTATAAAAAGGGGACAATGACTACAGGACACAATGTGGCT
NM_004515.3         GAAGTTCGACAGGTGGGATCCTATAAAAAGGGGACAATGACTACAGGACACAATGTGGCT
NM_001267809.1      GAAGTTCGACAGGTGGGATCCTATAAAAAGGGGACAATGACTACAGGACACAATGTGGCT
                    ************************************************************

HsCD00329821        GACCTGGTGGTGATACTCAAGATTCTGCCAACGTTGGAAGCTGTTGCTGCCCTGGGGAAC
NM_004515.3         GACCTGGTGGTGATACTCAAGATTCTGCCAACGTTGGAAGCTGTTGCTGCCCTGGGGAAC
NM_001267809.1      GACCTGGTGGTGATACTCAAGATTCTGCCAACGTTGGAAGCTGTTGCTGCCCTGGGGAAC
                    ************************************************************

HsCD00329821        AAAGTCGTGGAAAGCCTAAGAGCACAGGATCCTTCTGAAGTTTTAACCATGCTGACCAAC
NM_004515.3         AAAGTCGTGGAAAGCCTAAGAGCACAGGATCCTTCTGAAGTTTTAACCATGCTGACCAAC
NM_001267809.1      AAAGTCGTGGAAAGCCTAAGAGCACAGGATCCTTCTGAAGTTTTAACCATGCTGACCAAC
                    ************************************************************

HsCD00329821        GAAACTGGCTTTGAAATCAGTTCTTCTGATGCTACAGTGAAGATTCTCATTACAACAGTG
NM_004515.3         GAAACTGGCTTTGAAATCAGTTCTTCTGATGCTACAGTGAAGATTCTCATTACAACAGTG
NM_001267809.1      GAAACTGGCTTTGAAATCAGTTCTTCTGATGCTACAGTGAAGATTCTCATTACAACAGTG
                    ************************************************************

HsCD00329821        CCACCCAATCTTCGAAAACTGGATCCAGAACTCCATTTGGATATCAAAGTATTGCAGAGT
NM_004515.3         CCACCCAATCTTCGAAAACTGGATCCAGAACTCCATTTGGATATCAAAGTATTGCAGAGT
NM_001267809.1      CCACCCAATCTTCGAAAACTGGATCCAGAACTCCATTTGGATATCAAAGTATTGCAGAGT
                    ************************************************************

HsCD00329821        GCCTTAGCAGCCATCCGACATGCCCGCTGGTTCGAGGAAAATGCTTCTCAGTCCACAGTT
NM_004515.3         GCCTTAGCAGCCATCCGACATGCCCGCTGGTTCGAGGAAAATGCTTCTCAGTCCACAGTT
NM_001267809.1      GCCTTAGCAGCCATCCGACATGCCCGCTGGTTCGAGGAAAATGCTTCTCAGTCCACAGTT
                    ************************************************************

HsCD00329821        AAAGTTCTCATCAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGCCCCTC
NM_004515.3         AAAGTTCTCATCAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGCCCCTC
NM_001267809.1      AAAGTTCTCATCAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGCCCCTC
                    ************************************************************

HsCD00329821        ACACCCTGGATCCTTGACCTACTAGGCCATTATGCTGTGATGAACAACCCCACCAGACAG
NM_004515.3         ACACCCTGGATCCTTGACCTACTAGGCCATTATGCTGTGATGAACAACCCCACCAGACAG
NM_001267809.1      ACACCCTGGATCCTTGACCTACTAGGCCATTATGCTGTGATGAACAACCCCACCAGACAG
                    ************************************************************

HsCD00329821        CCTTTGGCCCTAAACGTTGCATACAGGCGCTGCTTGCAGATTCTGGCTGCAGGACTGTTC
NM_004515.3         CCTTTGGCCCTAAACGTTGCATACAGGCGCTGCTTGCAGATTCTGGCTGCAGGACTGTTC
NM_001267809.1      CCTTTGGCCCTAAACGTTGCATACAGGCGCTGCTTGCAGATTCTGGCTGCAGGACTGTTC
                    ************************************************************

HsCD00329821        CTGCCAGGTTCAGTGGGTATCACTGACCCCTGTGAGAGTGGCAACTTTAGAGTACACACA
NM_004515.3         CTGCCAGGTTCAGTGGGTATCACTGACCCCTGTGAGAGTGGCAACTTTAGAGTACACACA
NM_001267809.1      CTGCCAGGTTCAGTGGGTATCACTGACCCCTGTGAGAGTGGCAACTTTAGAGTACACACA
                    ************************************************************

HsCD00329821        GTCATGACCCTAGAACAGCAGGACATGGTCTGCTATACAGCTCAGACTCTCGTCCGAATC
NM_004515.3         GTCATGACCCTAGAACAGCAGGACATGGTCTGCTATACAGCTCAGACTCTCGTCCGAATC
NM_001267809.1      GTCATGACCCTAGAACAGCAGGACATGGTCTGCTATACAGCTCAGACTCTCGTCCGAATC
                    ************************************************************

HsCD00329821        CTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGGTGATGCCAGCTATCTTGCT
NM_004515.3         CTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGGTGATGCCAGCTATCTTGCT
NM_001267809.1      CTCTCACATGGTGGCTTTAGGAAGATCCTTGGCCAGGAGGGTGATGCCAGCTATCTTGCT
                    ************************************************************

HsCD00329821        TCTGAAATATCTACCTGGGATGGAGTGATAGTAACACCTTCAGAAAAGGCTTATGAGAAG
NM_004515.3         TCTGAAATATCTACCTGGGATGGAGTGATAGTAACACCTTCAGAAAAGGCTTATGAGAAG
NM_001267809.1      TCTGAAATATCTACCTGGGATGGAGTGATAGTAACACCTTCAGAAAAGGCTTATGAGAAG
                    ************************************************************

HsCD00329821        CCACCAGAGAAGAAGGAAGGAGAGGAAGAAGAGGAGAATACAGAAGAACCACCTCAAGGA
NM_004515.3         CCACCAGAGAAGAAGGAAGGAGAGGAAGAAGAGGAGAATACAGAAGAACCACCTCAAGGA
NM_001267809.1      CCACCAGAGAAGAAGGAAGGAGAGGAAGAAGAGGAGAATACAGAAGAACCACCTCAAGGA
                    ************************************************************

HsCD00329821        GAGGAAGAAGAAAGCATGGAAACTCAGGAGTTG
NM_004515.3         GAGGAAGAAGAAAGCATGGAAACTCAGGAGTGA
NM_001267809.1      GAGGAAGAAGAAAGCATGGAAACTCAGGAGTGA
                    ******************************* .

Pathways

WikiPathway : B Cell Receptor Signaling Pathway

Clone

Cloning Information : 269317
HIP Master Clone ID : 151497
Original Clone ID : FLH269317.01L

Organism


TAX_ID : 9606
Species Specific ID: 3608

Genome

Chromosome : 1
Map Location : 1q21.3
Ensembl : ENSG00000143621

Reagents

Labome : ILF2-antibody