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Explanation of Terms


Gene: Gene Symbol:  SPRED1
Gene Name:  sprouty-related, EVH1 domain containing 1
Sequence             
Original Clone ID: HR5538B-13-127-14.15
Keyword: targeted domain
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: PSI
Northeast Structural Genomics Consortium
HsCD00331904

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 351nts         Open reading frame : 1 to 351
Reference Sequence Alignment

ATGAGTTATGCACGAGTGCGAGCTGTGGTGATAACCCGAGATGACTCAAGTGGTGGATGG
TTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGTCCCTCATCAGGAA
GAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAAATGGTGGTT
TTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACATTTCACCAC
TGGAAGATTGATGACAAAAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGATGCTAGGGCT
TTTGATAGAGGTATCCGAAGAGCTATTGAGGATATTTCTCAAGGATGCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
g33a,M11I,a327t,I109I,
                                

5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in HR5538B
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : SPRED1
Symbol Nomenclature : SPRED1
SYNONYM : NFLS; hSpred1; spred-1
Designation : sprouty-related, EVH1 domain-containing protein 1|suppressor of Ras/MAPK activation
Full Nomenclature : sprouty-related, EVH1 domain containing 1
GENEID : 161742
HGNC : 20249
MIM : 609291
Vega : OTTHUMG00000172377
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00331904        ------------------------------------------------------------
NM_152594.2         ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAG----------------------
XM_005254202.1      ATGAGCGAGGAGACGGCGACTTCTGACAACGAGTGTCAGGGGCATGATGATGGAATCTGC
                                                                                

HsCD00331904        ---------ATGAGTTATGCACGAGTGCGAGCTGTGGTGATAACCCGAGATGACTCAAGT
NM_152594.2         --------------TTATGCACGAGTGCGAGCTGTGGTGATGACCCGAGATGACTCAAGT
XM_005254202.1      TTTTTTCATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACCCGAGATGACTCAAGT
                                  ***************************.******************

HsCD00331904        GGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGTCCCT
NM_152594.2         GGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGTCCCT
XM_005254202.1      GGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGTCCCT
                    ************************************************************

HsCD00331904        CATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAA
NM_152594.2         CATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAA
XM_005254202.1      CATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAA
                    ************************************************************

HsCD00331904        ATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACA
NM_152594.2         ATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACA
XM_005254202.1      ATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACA
                    ************************************************************

HsCD00331904        TTTCACCACTGGAAGATTGATGACAAAAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGAT
NM_152594.2         TTTCACCACTGGAAGATTGATGACAAGAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGAT
XM_005254202.1      TTTCACCACTGGAAGATTGATGACAAGAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGAT
                    **************************.*********************************

HsCD00331904        GCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATTGAGGATATTTCTCAAGGATGCTAG
NM_152594.2         GCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATAGAGGATATTTCTCAAGGATGCCCC
XM_005254202.1      GCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATAGAGGATATTTCTCAAGGATGCCCC
                    ***********************************:********************* . 

HsCD00331904        ------------------------------------------------------------
NM_152594.2         GAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTACAAGCAAATGAAGAGGATTCTTCC
XM_005254202.1      GAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTACAAGCAAATGAAGAGGATTCTTCC
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         AGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAGACAGTTGTTACCAGTGAGCCTTAT
XM_005254202.1      AGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAGACAGTTGTTACCAGTGAGCCTTAT
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         AGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGATCTGAATGCCAGAAGAGTCTACATG
XM_005254202.1      AGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGATCTGAATGCCAGAAGAGTCTACATG
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         CAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCAGGCTTGGACATTCAGAGCAGAAGT
XM_005254202.1      CAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCAGGCTTGGACATTCAGAGCAGAAGT
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         ATGGAATACGTACAGCGGCAAATATCCAAGGAATGTGGAAGCCTAAAGTCCCAAAATAGG
XM_005254202.1      ATGGAATACGTACAGCGGCAAATATCCAAGGAATGTGGAAGCCTAAAGTCCCAAAATAGG
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         GTCCCTTTGAAATCAATCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTCAGAATA
XM_005254202.1      GTCCCTTTGAAATCAATCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTCAGAATA
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         AACCCTCGAGATATCTTAATACGTCGCTATGCAGACTACAGACATCCTGACATGTGGAAA
XM_005254202.1      AACCCTCGAGATATCTTAATACGTCGCTATGCAGACTACAGACATCCTGACATGTGGAAA
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         AATGACTTGGAAAGAGATGATGCTGATTCCAGTATTCAGTTTTCTAAACCAGACAGTAAA
XM_005254202.1      AATGACTTGGAAAGAGATGATGCTGATTCCAGTATTCAGTTTTCTAAACCAGACAGTAAA
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         AAATCAGACTATCTGTACTCTTGTGGGGATGAGACTAAGTTAAGTTCACCCAAAGACTCT
XM_005254202.1      AAATCAGACTATCTGTACTCTTGTGGGGATGAGACTAAGTTAAGTTCACCCAAAGACTCT
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         GTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATTAAGAAGTCAAAACGAAGAAAAGAG
XM_005254202.1      GTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATTAAGAAGTCAAAACGAAGAAAAGAG
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         GATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAATGTT
XM_005254202.1      GATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAATGTT
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         AGGGGAAAATGTCAGGATGCTCCAGACCCTATTAAAAGATGCATATATCAAGTTAGTTGC
XM_005254202.1      AGGGGAAAATGTCAGGATGCTCCAGACCCTATTAAAAGATGCATATATCAAGTTAGTTGC
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         ATGCTCTGTGCAGAGAGCATGTTGTATCATTGTATGTCAGACTCAGAGGGAGATTTTTCT
XM_005254202.1      ATGCTCTGTGCAGAGAGCATGTTGTATCATTGTATGTCAGACTCAGAGGGAGATTTTTCT
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         GATCCCTGTTCGTGTGACACTAGCGACGACAAGTTCTGCTTGCGATGGTTAGCCCTGGTA
XM_005254202.1      GATCCCTGTTCGTGTGACACTAGCGACGACAAGTTCTGCTTGCGATGGTTAGCCCTGGTA
                                                                                

HsCD00331904        ------------------------------------------------------------
NM_152594.2         GCTTTGTCTTTCATTGTACCATGTATGTGCTGCTACGTCCCTTTGAGAATGTGCCATCGC
XM_005254202.1      GCTTTGTCTTTCATTGTACCATGTATGTGCTGCTACGTCCCTTTGAGAATGTGCCATCGC
                                                                                

HsCD00331904        ---------------------------------------------------
NM_152594.2         TGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACATAAAGCTGCTGGATGA
XM_005254202.1      TGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACATAAAGCTGCTGGATGA
                                                                       

Protein

PDB - Structural Annotation : 3SYX
SMART domain : WH1 : WASP homology region 1
Structural Annotation Wiki : TOPSAN
Swissprot ID : SPRE1_HUMAN
HPRD : 11601
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 536863
HIP Master Clone ID : 447183
Original Clone ID : HR5538B-13-127-14.15

Experimental

Structural Biology Knowledgebase : SBKB (3SYX)
TargetTrack - Experimental data : HR5538B

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 15
Map Location : 15q14
Ensembl : ENSG00000166068