Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name:  None
Original Clone ID:
11023l1BCt8p1
Keyword:
None
Species:
Hahella chejuensis KCTC 2396
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 21387413
Title: Structural basis for a ribofuranosyl binding protein: insights into the furanose specific transport.
Authors:
PSI
New York SGX Research Center for Structural Genomics
HcCD00342063
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 855nts
Open reading frame: 1 to 855

CAGCCGAAACTGCTGCTGGTCCTGAAAGGCGATTCAAACGCATATTGGCGTCAGGTGTAC
CTGGGCGCCCAAAAAGCGGCCGATGAAGCAGGTGTGACCCTGCTGCATCGCTCGACGAAA
GATGACGGCGACATTGCCGGTCAGATTCAAATCCTGAGTTATCACCTGTCCCAGGCACCG
CCGGATGCACTGATCCTGGCACCGAACTCAGCTGAAGACCTGACCCCGTCGGTGGCACAG
TACCGTGCACGCAATATCCCGGTTCTGGTGGTTGATAGTGACCTGGCGGGCGATGCCCAT
CAGGGTCTGGTTGCGACCGACAACTATGCAGCTGGTCAACTGGCAGCCCGTGCACTGCTG
GCTACGCTGGATCTGAGCAAAGAACGCAACATTGCACTGCTGCGTCTGCGTGCCGGTAAT
GCCTCTACCGATCAGCGTGAACAAGGTTTTCTGGATGTCCTGCGTAAACACGACAAAATC
CGCATTATCGCAGCTCCGTACGCGGGTGATGACCGTGGTGCAGCCCGCAGTGAAATGCTG
CGTCTGCTGAAAGAAACCCCGACGATTGATGGTCTGTTTACCCCGAATGAATCCACCACG
ATTGGCGCACTGGTGGCTATCCGCCAGAGCGGCATGTCTAAACAATTTGGCTTCATTGGT
TTCGATCAGACGGAAGAACTGGAAGCAGCTATGTATGCGGGCGAAATCTCTAACCTGGTC
GTGCAGAATCCGGAATATATGGGTTACCTGGCAGTTCAACGTGCTCTGGATCTGGTCCGC
GGCAAACCGATTCCGGCGTTCACCGACACGGGTGTTCGCCTGCTGCAGGAAGGGCATCAT
CACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

QPKLLLVLKGDSNAYWRQVYLGAQKAADEAGVTLLHRSTKDDGDIAGQIQILSYHLSQAP
PDALILAPNSAEDLTPSVAQYRARNIPVLVVDSDLAGDAHQGLVATDNYAAGQLAARALL
ATLDLSKERNIALLRLRAGNASTDQREQGFLDVLRKHDKIRIIAAPYAGDDRGAARSEML
RLLKETPTIDGLFTPNESTTIGALVAIRQSGMSKQFGFIGFDQTEELEAAMYAGEISNLV
VQNPEYMGYLAVQRALDLVRGKPIPAFTDTGVRLLQEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11023l
Recommended expression in:
Not Applicable