BT_3528 (B. thetaiotaomicron )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_3528
Gene Name:  hypothetical protein
Original Clone ID:
BtR60-21.3
Keyword:
None
Species:
Bacteroides thetaiotaomicron
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
BtCD00342891
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1461nts
Open reading frame: 1 to 1461

ATGAAGAAGCAAGTAAAATATATCAGTGCGGGTATGCTGGCAGGCATGCTCCTTTGTGGC
GGTCAGATGCAGGCTGCAAATACGATGTCCGGAATGCATGTATGTGTGACGGACGCTATT
CAGAAAGACAATCGTCCGGAAGTTTCCAAACGTCTTTTCCGTTCGAATGCGGTAGAAAAG
GAAATCATCCGTGTGCAGAAACTTCTGAAAAACGAGAAACTGGCATGGATGTTCGCAAAC
TGTTTCCCCAATACCATCGATACTACCGTACACTTCCGCAAAGGCGCGGATGGCAAACCG
GATACATTCGTTTATACGGGCGACATTCACGCAATGTGGCTTCGTGACTCGGGTGCGCAG
GTATGGCCGTATGTGCAGTTGGCAAACTCCGATCCCGAACTGAAAGAGATGCTGGCAGGT
GTTATCCTCCGCCAGTTCAAATGTATCAATATCGATCCGTATGCAAACGCATTTAATGAC
GGTGCCGTTGAAGACAATCACTGGATGAGTGACCTGACCGATATGAAGCCGGAACTGCAC
GAGCGTAAATGGGAAATCGATTCACTTTGCTACCCGCTGCGTCTGGCTTATCACTACTGG
AAAACAACCGGAGATGCCAGCATCTTCTCAGAAGAATGGATTCAGGCTATCACCAATGTG
CTGAAAACATTCAAGGAACAACAGCGCAAAGACGGAGTTGGCCCGTATAAATTCCAGCGT
AAGACAGAACGTGCATTGGATACATTGAATAATGACGGACTCGGAGCACCAGTGAAACCG
GTTGGTCTGATCGTCTCCTGCTTCCGTCCTTCGGATGATGCGACGACTCTTCAATATCTG
GTACCTTCCAATTTCTTTGCCGTATCTTCATTGCGCAAAGCAGCCGAAATACTTGATAAG
GTAAATAAGAAGACTGCTTTGGCTAAGGAATGCAAAGACCTGGCAAAGGAAGTAGAGACA
GCATTGAAGAAATATGCGGTATACAACCACCCGAAATATGGTAAAATCTATGCTTTTGAG
GTAGACGGCTTCGGAAATCACTTCCTGATGGATGATGCCAATGTACCGAGCCTGCTTGCC
ATGCCTTATCTGGGTGATGTAGATGTAAACGATCCTATCTATCAGAATACCCGTAAATTC
GTGTGGAGCGAAGACAATCCTTACTTCTTCAAAGGCAAGGCAGGCGAAGGTATCGGCGGA
CCGCATATCGGTTATGATATGGTGTGGCCGATGAGTATCATGATGAAAGCCTTCACCAGC
AAGGATGATGCGGAAATCAAAACCTGTATCAAAATGCTGATGGATACCGATGCCGGAACA
GGCTTTATGCATGAATCTTTCCACAAAGACGATCCGAAGAACTTCACCCGTGCCTGGTTT
GCCTGGCAGAATACGCTTTTCGGAGAACTGATTCTTAAGTTGGTTAACGAAGGCAAAGTA
GATTTATTGAATAGTATCAAT

Protein sequence predicted based on nucleotide sequence

MKKQVKYISAGMLAGMLLCGGQMQAANTMSGMHVCVTDAIQKDNRPEVSKRLFRSNAVEK
EIIRVQKLLKNEKLAWMFANCFPNTIDTTVHFRKGADGKPDTFVYTGDIHAMWLRDSGAQ
VWPYVQLANSDPELKEMLAGVILRQFKCINIDPYANAFNDGAVEDNHWMSDLTDMKPELH
ERKWEIDSLCYPLRLAYHYWKTTGDASIFSEEWIQAITNVLKTFKEQQRKDGVGPYKFQR
KTERALDTLNNDGLGAPVKPVGLIVSCFRPSDDATTLQYLVPSNFFAVSSLRKAAEILDK
VNKKTALAKECKDLAKEVETALKKYAVYNHPKYGKIYAFEVDGFGNHFLMDDANVPSLLA
MPYLGDVDVNDPIYQNTRKFVWSEDNPYFFKGKAGEGIGGPHIGYDMVWPMSIMMKAFTS
KDDAEIKTCIKMLMDTDAGTGFMHESFHKDDPKNFTRAWFAWQNTLFGELILKLVNEGKV
DLLNSIN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAGGAGATATACAT
3' Linker Sequence:
CTCGAGCACCACCACCACCACCACTGA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in BtR60
Recommended expression in:
Not Applicable