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CDC37 (Homo sapiens) in pENTR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CDC37
Gene Name:  cell division cycle 37
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100002242)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00351475

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1134nts         Open reading frame : 1 to 1134
Reference Sequence Alignment

ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
CTGGAGCGCCAGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCC
ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACCCTGGC
CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR201

pENTR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments: derived from pDONR201, available from Invitrogen Corp. More information can be found here http://orfeomecollaboration.org/vectors.php
Size (bp): 2294
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC bacterial origin of replication 1629 2268
primer pDONR201-forward pDONR201-forward sequencing primer GTAACATCAGAGATTTTGAGACAC 570 593
primer pDONR201-reverse pDONR201-reverse sequencing primer TCGCGTTAACGCTAGCATGGATCTC 307 331
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 488 576
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 418 338
selectable marker KanR Kanamycin resistance gene 699 1508
trxn termination sequence rrnB T1-T2 rrnb RNA termination 322 56

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDC37
Symbol Nomenclature : CDC37
SYNONYM : P50CDC37
Designation : CDC37 (cell division cycle 37, S. cerevisiae, homolog)|CDC37 cell division cycle 37 homolog|cell division cycle 37 homolog|hsp90 chaperone protein kinase-targeting subunit|hsp90 co-chaperone Cdc37
Full Nomenclature : cell division cycle 37
GENEID : 11140
GenBank Accession : 564082
HGNC : 1735
MIM : 605065
Vega : OTTHUMG00000180578
Target GenBank: AM393285

 

Reference Sequence Alignment

  

HsCD00351475      ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
NM_007065.3       ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
                  ************************************************************

HsCD00351475      CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
NM_007065.3       CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
                  ************************************************************

HsCD00351475      GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
NM_007065.3       GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
                  ************************************************************

HsCD00351475      GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
NM_007065.3       GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
                  ************************************************************

HsCD00351475      CTGGAGCGCCAGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
NM_007065.3       CTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
                  **********:*************************************************

HsCD00351475      AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
NM_007065.3       AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
                  ************************************************************

HsCD00351475      AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
NM_007065.3       AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
                  ************************************************************

HsCD00351475      GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
NM_007065.3       GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
                  ************************************************************

HsCD00351475      CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
NM_007065.3       CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
                  ************************************************************

HsCD00351475      CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
NM_007065.3       CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
                  ************************************************************

HsCD00351475      GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
NM_007065.3       GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
                  ************************************************************

HsCD00351475      GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
NM_007065.3       GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
                  ************************************************************

HsCD00351475      ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
NM_007065.3       ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
                  ************************************************************

HsCD00351475      GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
NM_007065.3       GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
                  ************************************************************

HsCD00351475      GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
NM_007065.3       GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
                  ************************************************************

HsCD00351475      CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCC
NM_007065.3       CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCC
                  ************************************************************

HsCD00351475      ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACCCTGGC
NM_007065.3       ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGC
                  ****************************************************** *****

HsCD00351475      CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
NM_007065.3       CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
                  ************************************************************

HsCD00351475      GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTG---
NM_007065.3       GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTGTGA
                  ******************************************************   

Pathways

NCI : Integrin-linked kinase signaling
NCI : LKB1 signaling events
Reactome : Disease
Reactome : Signal Transduction
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by constitutively active EGFR
WikiPathway : TNF alpha Signaling Pathway

Clone

Cloning Information : 564082

Organism


TAX_ID : 9606
Species Specific ID: 11140

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000105401

Reagents

Labome : CDC37-antibody