Gene:
Gene Symbol:
PIK3AP1
Gene Name:
phosphoinositide-3-kinase adaptor protein 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100002318)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00351557
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCAGCCTCAGGGGTGCCCAGAGGATGCGACATCCTCATCGTCTACAGCCCGGATGCC
GAGGAATGGTGCCAGTACCTGCAGACCCTGTTCCTGTCCAGTCGGCAGGTCCGCAGCCAG
AAGATACTGACTCACAGGCTGGGCCCCGAGGCCTCCTTCTCGGCAGAGGACCTAAGCCTT
TTCCTCAGCACCCGCTGTGTCGTGGTGCTGCTGTCCGCGGAGCTGGTGCAGCACTTCCAC
AAGCCCTCCTTGCTGCCCCTGCTGCAGAGAGCTTTCCATCCTCCGCACCGCGTGGTCAGG
CTGCTCTGCGGCGTGCGGGACAGCGAGGAGTTCCTAGACTTCTTTCCAGATTGGGCCCAT
TGGCAGGAGCTCACCTGTGACGATGAGCCAGAGACCTACGTGGCAGCTGTGAAAAAAGCC
ATTTCCGAAGATTCTGGCTGTGACTCAGTCACTGACACTGAGCCTGAGGACGAGAAGGTT
GTTTCCTACTCGAAGCAGCAGAACCTGCCGACGGTGACTTCACCTGGGAACCTGATGGTG
GTGCAGCCGGACCGCATTCGCTGTGGGGCAGAAACCACTGTCTATGTTATTGTGAGATGT
AAGCTGGATGACAGGGTGGCGACAGAAGCCGAGTTTTCTCCTGAGGATTCTCCCTCTGTA
AGGATGGAAGCCAAGGTGGAGAATGAGTACACCATTTCAGTGAAGGCTCCCAACCTTTCA
TCTGGGAACGTTTCTCTGAAGATATATTCTGGAGACTTAGTGGTGTGTGAAACCGTTATC
AGCTATTATACTGACATGGAAGAAATTGGGAATTTATTGTCCAATGCCGCGAATCCTGTG
GAATTCATGTGTCAGGCCTTTAAAATTGTGCCCTACAACACAGAGACCCTTGATAAACTG
CTAACCGAATCCCTGAAGAACAATATCCCTGCAAGCGGACTGCACCTCTTTGGAATCAAC
CAGCTGGAAGAAGAAGATATGATGACAAATCAGAGGGATGAAGAGCTGCCCACCCTGTTG
CATTTTGCTGCGAAGTATGGACTGAAGAACCTCACTGCCTTGTTGCTCACCTGCCCAGGA
GCCCTGCAGGCGTACAGCGTGGCCAACAAGCATGGCCACTACCCCAACACCATCGCTGAG
AAACACGGCTTCAGGGACCTGCGGCAGTTCATCGACGAGTATGTGGAAACGGTGGACATG
CTCAAGAGTCACATTAAAGAGGAACTGATGCACGGGGAGGAGGCTGATGCTGTGTACGAG
TCCATGGCCCACCTTTCCACAGACCTGCTTATGAAATGCTCGCTCAACCCCGGCTGTGAC
GAGGATCTCTATGAGTCCATGGCTGCCTTTGTCCCAGCTGCCACTGAAGACCTCTATGTT
GAAATGCTTCAGGCCAGTACATCTAACCCAATCCCTGGAGATGGTTTCTCTCGGGCCACT
AAGGACTCTATGATCCGCAAGTTTTTAGAAGGCAACAGCATGGGAATGACCAATCTGGAG
AGAGATCAGTGCCATCTTGGTCAGGAAGAAGATGTTTATCACACGGTGGATGACGATGAG
GCCTTTTCTGTGGACCTGGCCAGCAGGCCCCCTGTCCCAGTGCCCAGACCAGAGACCACT
GCTCCTGGTGCTCACCAGCTGCCTGACAACGAACCATACATTTTTAAAGTTTTTGCAGAA
AAAAGTCAAGAGCGGCCTGGGAATTTCTACGTTTCCTCAGAGAGCATCAGGAAAGGGCCG
CCCGTCAGACCATGGAGGGACAGGCCCCAGTCAAGTATATATGACCCTTTTGCGGGAATG
AAAACGCCAGGCCAGCGGCAGCTTATCACCCTCCAGGAGCAGGTGAAGCTGGGCATTGTC
AACGTGGATGAGGCTGTGCTCCACTTCAAAGAGTGGCAGCTCAACCAGAAGAAACGATCG
GAGTCCTTTCGTTTCCAGCAGGAAAATCTTAAACGGCTAAGAGACAGCATCACCCGAAGA
CAGAGAGAGAAGCAAAAATCAGGAAAGCAGACAGACTTGGAGATCACGGTCCCAATTCGG
CACTCACAGCACCTGCCTGCAAAAGTGGAGTTTGGAGTCTATGAGAGTGGCCCCAGGAAA
AGTGTCATTCCCCCTAGGACGGAGCTGAGACGAGGAGACTGGAAAACAGACAGCACCTCC
AGCACAGCAAGTAGCACAAGTAACCGCTCCAGCACCCGGAGCCTCCTCAGTGTGAGCAGC
GGGATGGAAGGGGACAACGAGGATAATGAAGTCCCTGAGGTTACCAGAAGTCGCAGTCCA
GGCCCCCCACAAGTGGATGGGACACCCACCATGTCCCTCGAGAGACCCCCCAGGGTGCCT
CCGAGAGCTGCCTCACAGAGGCCTCCGACCAGGGAGACCTTCCATCCTCCTCCACCTGTT
CCACCCAGAGGACGC
Protein sequence predicted based on nucleotide sequence
MAASGVPRGCDILIVYSPDAEEWCQYLQTLFLSSRQVRSQKILTHRLGPEASFSAEDLSL FLSTRCVVVLLSAELVQHFHKPSLLPLLQRAFHPPHRVVRLLCGVRDSEEFLDFFPDWAH WQELTCDDEPETYVAAVKKAISEDSGCDSVTDTEPEDEKVVSYSKQQNLPTVTSPGNLMV VQPDRIRCGAETTVYVIVRCKLDDRVATEAEFSPEDSPSVRMEAKVENEYTISVKAPNLS SGNVSLKIYSGDLVVCETVISYYTDMEEIGNLLSNAANPVEFMCQAFKIVPYNTETLDKL LTESLKNNIPASGLHLFGINQLEEEDMMTNQRDEELPTLLHFAAKYGLKNLTALLLTCPG ALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKSHIKEELMHGEEADAVYE SMAHLSTDLLMKCSLNPGCDEDLYESMAAFVPAATEDLYVEMLQASTSNPIPGDGFSRAT KDSMIRKFLEGNSMGMTNLERDQCHLGQEEDVYHTVDDDEAFSVDLASRPPVPVPRPETT APGAHQLPDNEPYIFKVFAEKSQERPGNFYVSSESIRKGPPVRPWRDRPQSSIYDPFAGM KTPGQRQLITLQEQVKLGIVNVDEAVLHFKEWQLNQKKRSESFRFQQENLKRLRDSITRR QREKQKSGKQTDLEITVPIRHSQHLPAKVEFGVYESGPRKSVIPPRTELRRGDWKTDSTS STASSTSNRSSTRSLLSVSSGMEGDNEDNEVPEVTRSRSPGPPQVDGTPTMSLERPPRVP PRAASQRPPTRETFHPPPPVPPRGR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments:
derived from pDONR201, available from Invitrogen Corp. More information can be found here http://orfeomecollaboration.org/vectors.php
Size (bp):
2294
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin of replication
1629
2268
primer
pDONR201-forward
pDONR201-forward sequencing primer GTAACATCAGAGATTTTGAGACAC
570
593
primer
pDONR201-reverse
pDONR201-reverse sequencing primer TCGCGTTAACGCTAGCATGGATCTC
307
331
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only
418
338
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only
488
576
selectable marker
KanR
Kanamycin resistance gene
699
1508
trxn termination sequence
rrnB
T1-T2 rrnb RNA termination
322
56
