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SFXN4 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SFXN4
Gene Name:  sideroflexin 4
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100071442)
Mutation/
Discrepancy :
No / No
Publications: PMID: 24119684
Title: Macrocytic Anemia and Mitochondriopathy Resulting from a Defect in Sideroflexin.
Authors: The ORFeome Collaboration
HsCD00352377

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1011nts         Open reading frame : 1 to 1011
Reference Sequence Alignment

ATGTCCCTGGAACAGGAGGAGGAAACGCAACCTGGGCGGCTCCTAGGACGCAGAGACGCC
GTCCCCGCCTTCATTGAGCCCAACGTGCGCTTCTGGATCACCGAGCGCCAATCCTTTATT
CGACGATTTCTTCAATGGACAGAATTATTAGATCCTACAAATGTGTTCATTTCAGTTGAA
AGTATAGAAAACTCGAGGCAACTATTGTGCACAAATGAAGATGTTTCCAGCCCTGCCTCG
GCGGACCAAAGGATACAAGAAGCTTGGAAGCGGAGTCTTGCAACAGTGCATCCCGACAGC
AGCAACCTGATCCCCAAGCTTTTTCGACCTGCAGCGTTCCTGCCTTTCATGGCGCCCACG
GTATTTTTGTCAATGACGCCACTGAAAGGGATCAAGTCCGTGATTTTACCTCAGGTTTTC
CTCTGTGCCTACATGGCAGCGTTCAACAGCATCAATGGAAACAGAAGTTACACTTGTAAG
CCACTAGAAAGATCATTACTAATGGCGGGAGCCGTTGCTTCTTCAACTTTCTTAGGAGTA
ATCCCTCAGTTTGTCCAGATGAAGTATGGCCTGACTGGCCCTTGGATTAAAAGACTCTTA
CCTGTGATCTTCCTCGTGCAAGCCAGTGGAATGAATGTCTACATGTCCCGAAGTCTTGAA
TCCATTAAGGGGATTGCGGTCATGGACAAGGAAGGCAATGTCCTGGGTCATTCCAGAATT
GCTGGGACAAAGGCTGTTAGAGAAACGCTAGCATCCAGAATAGTGCTGTTTGGGACCTCA
GCTCTGATTCCTGAAGTCTTCACCTACTTTTTTAAAAGGACCCAGTATTTCAGGAAAAAC
CCAGGGTCATTGTGGATTTTGAAACTGTCTTGTACTGTCCTGGCAATGGGACTGATGGTG
CCATTTTCTTTTAGTATATTTCCACAGATTGGACAGATACAGTACTGTAGTCTTGAAGAG
AAAATTCAGTCTCCAACAGAAGAAACAGAAATCTTTTATCACAGAGGGGTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SFXN4
Symbol Nomenclature : SFXN4
SYNONYM : BCRM1; COXPD18
Designation : breast cancer resistance marker 1|sideroflexin-4
Full Nomenclature : sideroflexin 4
GENEID : 119559
GenBank Accession : 564984
HGNC : 16088
MIM : 615564
Vega : OTTHUMG00000019147
Target GenBank: HQ448063

 

Reference Sequence Alignment

  

HsCD00352377      ATGTCCCTGGAACAGGAGGAGGAAACGCAACCTGGGCGGCTCCTAGGACGCAGAGACGCC
NM_213649.1       ATGTCCCTGGAACAGGAGGAGGAAACGCAACCTGGGCGGCTCCTAGGACGCAGAGACGCC
                  ************************************************************

HsCD00352377      GTCCCCGCCTTCATTGAGCCCAACGTGCGCTTCTGGATCACCGAGCGCCAATCCTTTATT
NM_213649.1       GTCCCCGCCTTCATTGAGCCCAACGTGCGCTTCTGGATCACCGAGCGCCAATCCTTTATT
                  ************************************************************

HsCD00352377      CGACGATTTCTTCAATGGACAGAATTATTAGATCCTACAAATGTGTTCATTTCAGTTGAA
NM_213649.1       CGACGATTTCTTCAATGGACAGAATTATTAGATCCTACAAATGTGTTCATTTCAGTTGAA
                  ************************************************************

HsCD00352377      AGTATAGAAAACTCGAGGCAACTATTGTGCACAAATGAAGATGTTTCCAGCCCTGCCTCG
NM_213649.1       AGTATAGAAAACTCGAGGCAACTATTGTGCACAAATGAAGATGTTTCCAGCCCTGCCTCG
                  ************************************************************

HsCD00352377      GCGGACCAAAGGATACAAGAAGCTTGGAAGCGGAGTCTTGCAACAGTGCATCCCGACAGC
NM_213649.1       GCGGACCAAAGGATACAAGAAGCTTGGAAGCGGAGTCTTGCAACAGTGCATCCCGACAGC
                  ************************************************************

HsCD00352377      AGCAACCTGATCCCCAAGCTTTTTCGACCTGCAGCGTTCCTGCCTTTCATGGCGCCCACG
NM_213649.1       AGCAACCTGATCCCCAAGCTTTTTCGACCTGCAGCGTTCCTGCCTTTCATGGCGCCCACG
                  ************************************************************

HsCD00352377      GTATTTTTGTCAATGACGCCACTGAAAGGGATCAAGTCCGTGATTTTACCTCAGGTTTTC
NM_213649.1       GTATTTTTGTCAATGACGCCACTGAAAGGGATCAAGTCCGTGATTTTACCTCAGGTTTTC
                  ************************************************************

HsCD00352377      CTCTGTGCCTACATGGCAGCGTTCAACAGCATCAATGGAAACAGAAGTTACACTTGTAAG
NM_213649.1       CTCTGTGCCTACATGGCAGCGTTCAACAGCATCAATGGAAACAGAAGTTACACTTGTAAG
                  ************************************************************

HsCD00352377      CCACTAGAAAGATCATTACTAATGGCGGGAGCCGTTGCTTCTTCAACTTTCTTAGGAGTA
NM_213649.1       CCACTAGAAAGATCATTACTAATGGCGGGAGCCGTTGCTTCTTCAACTTTCTTAGGAGTA
                  ************************************************************

HsCD00352377      ATCCCTCAGTTTGTCCAGATGAAGTATGGCCTGACTGGCCCTTGGATTAAAAGACTCTTA
NM_213649.1       ATCCCTCAGTTTGTCCAGATGAAGTATGGCCTGACTGGCCCTTGGATTAAAAGACTCTTA
                  ************************************************************

HsCD00352377      CCTGTGATCTTCCTCGTGCAAGCCAGTGGAATGAATGTCTACATGTCCCGAAGTCTTGAA
NM_213649.1       CCTGTGATCTTCCTCGTGCAAGCCAGTGGAATGAATGTCTACATGTCCCGAAGTCTTGAA
                  ************************************************************

HsCD00352377      TCCATTAAGGGGATTGCGGTCATGGACAAGGAAGGCAATGTCCTGGGTCATTCCAGAATT
NM_213649.1       TCCATTAAGGGGATTGCGGTCATGGACAAGGAAGGCAATGTCCTGGGTCATTCCAGAATT
                  ************************************************************

HsCD00352377      GCTGGGACAAAGGCTGTTAGAGAAACGCTAGCATCCAGAATAGTGCTGTTTGGGACCTCA
NM_213649.1       GCTGGGACAAAGGCTGTTAGAGAAACGCTAGCATCCAGAATAGTGCTGTTTGGGACCTCA
                  ************************************************************

HsCD00352377      GCTCTGATTCCTGAAGTCTTCACCTACTTTTTTAAAAGGACCCAGTATTTCAGGAAAAAC
NM_213649.1       GCTCTGATTCCTGAAGTCTTCACCTACTTTTTTAAAAGGACCCAGTATTTCAGGAAAAAC
                  ************************************************************

HsCD00352377      CCAGGGTCATTGTGGATTTTGAAACTGTCTTGTACTGTCCTGGCAATGGGACTGATGGTG
NM_213649.1       CCAGGGTCATTGTGGATTTTGAAACTGTCTTGTACTGTCCTGGCAATGGGACTGATGGTG
                  ************************************************************

HsCD00352377      CCATTTTCTTTTAGTATATTTCCACAGATTGGACAGATACAGTACTGTAGTCTTGAAGAG
NM_213649.1       CCATTTTCTTTTAGTATATTTCCACAGATTGGACAGATACAGTACTGTAGTCTTGAAGAG
                  ************************************************************

HsCD00352377      AAAATTCAGTCTCCAACAGAAGAAACAGAAATCTTTTATCACAGAGGGGTG---
NM_213649.1       AAAATTCAGTCTCCAACAGAAGAAACAGAAATCTTTTATCACAGAGGGGTGTAG
                  ***************************************************   

Protein

UniProt : Q6P4A7
HPRD : 18046

Clone

Cloning Information : 564984

Organism


TAX_ID : 9606
Species Specific ID: 119559

Genome

Chromosome : 10
Map Location : 10q26.11
Ensembl : ENSG00000183605

Reagents

Labome : SFXN4-antibody