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CYP2U1 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP2U1
Gene Name:  cytochrome P450, family 2, subfamily U, polypeptide 1
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100073170)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00353719

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1632nts         Open reading frame : 1 to 1632
Reference Sequence Alignment

ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
AAGGAGAAGGGGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTC
TCTCATTCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATT
GAGGAGTTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCT
TTCTCCATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGC
TTTGATTACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAA
ATCTGTCTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCC
TTTGGACCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAA
ATCATCAAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATG
TACCTTCTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAG
TACTTATTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCT
TTGCTCTGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAA
GAAATTGAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATG
CCCTACACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCC
ATTCCTCATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACA
TTGATCTTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAG
GATTTCTACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTT
ATTCCTTTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTA
TTCCTAATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAG
AAGCCCCTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACT
ATTTCAAGGAGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP2U1
Symbol Nomenclature : CYP2U1
SYNONYM : P450TEC; SPG49; SPG56
Designation : cytochrome P450 2U1|spastic paraplegia 49
Full Nomenclature : cytochrome P450, family 2, subfamily U, polypeptide 1
GENEID : 113612
GenBank Accession : 571721
HGNC : 20582
MIM : 610670
Vega : OTTHUMG00000161084
Target GenBank: HQ258621

 

Reference Sequence Alignment

  

HsCD00353719        ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
NM_183075.2         ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
XM_005262717.1      ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
                    ************************************************************

HsCD00353719        CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
NM_183075.2         CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
XM_005262717.1      CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
                    ************************************************************

HsCD00353719        GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
NM_183075.2         GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
XM_005262717.1      GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
                    ************************************************************

HsCD00353719        CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
NM_183075.2         CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
XM_005262717.1      CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
                    ************************************************************

HsCD00353719        CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
NM_183075.2         CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
XM_005262717.1      CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
                    ************************************************************

HsCD00353719        ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
NM_183075.2         ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
XM_005262717.1      ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
                    ************************************************************

HsCD00353719        TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
NM_183075.2         TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
XM_005262717.1      TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
                    ************************************************************

HsCD00353719        GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
NM_183075.2         GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
XM_005262717.1      GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
                    ************************************************************

HsCD00353719        AAGGAGAAGG--------------------------------------------------
NM_183075.2         AAGGAGAAGG--------------------------------------------------
XM_005262717.1      AAGGAGAAGGGTGAGCGGGAGGTCGTGGGCTGTGGGTACGCGGATGCCGCGGATGAGTCT
                    **********                                                  

HsCD00353719        ----GGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
NM_183075.2         ----GGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
XM_005262717.1      CCAGGGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
                        ********************************************************

HsCD00353719        TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
NM_183075.2         TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
XM_005262717.1      TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
                    ************************************************************

HsCD00353719        TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
NM_183075.2         TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
XM_005262717.1      TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
                    ************************************************************

HsCD00353719        ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
NM_183075.2         ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
XM_005262717.1      ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
                    ************************************************************

HsCD00353719        TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
NM_183075.2         TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
XM_005262717.1      TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
                    ************************************************************

HsCD00353719        CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
NM_183075.2         CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
XM_005262717.1      CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
                    ************************************************************

HsCD00353719        CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
NM_183075.2         CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
XM_005262717.1      CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
                    ************************************************************

HsCD00353719        AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
NM_183075.2         AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
XM_005262717.1      AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
                    ************************************************************

HsCD00353719        CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
NM_183075.2         CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
XM_005262717.1      CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
                    ************************************************************

HsCD00353719        TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
NM_183075.2         TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
XM_005262717.1      TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
                    ************************************************************

HsCD00353719        TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
NM_183075.2         TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
XM_005262717.1      TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
                    ************************************************************

HsCD00353719        GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
NM_183075.2         GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
XM_005262717.1      GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
                    ************************************************************

HsCD00353719        ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
NM_183075.2         ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
XM_005262717.1      ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
                    ************************************************************

HsCD00353719        CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
NM_183075.2         CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
XM_005262717.1      CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
                    ************************************************************

HsCD00353719        TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
NM_183075.2         TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
XM_005262717.1      TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
                    ************************************************************

HsCD00353719        TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
NM_183075.2         TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
XM_005262717.1      TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
                    ************************************************************

HsCD00353719        TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
NM_183075.2         TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
XM_005262717.1      TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
                    ************************************************************

HsCD00353719        ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
NM_183075.2         ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
XM_005262717.1      ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
                    ************************************************************

HsCD00353719        CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
NM_183075.2         CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
XM_005262717.1      CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
                    ************************************************************

HsCD00353719        AGGAGA---
NM_183075.2         AGGAGATGA
XM_005262717.1      AGGAGATGA
                    ******   

Pathways

Reactome : Arachidonic acid metabolism
Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

UniProt : Q7Z449
HPRD : 13109

Clone

Cloning Information : 571721

Organism


TAX_ID : 9606
Species Specific ID: 113612

Genome

Chromosome : 4
Map Location : 4q25
Ensembl : ENSG00000155016

Reagents

Labome : CYP2U1-antibody