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GABRR2 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GABRR2
Gene Name:  gamma-aminobutyric acid (GABA) A receptor, rho 2
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072868)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00353766

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1395nts         Open reading frame : 1 to 1395
Reference Sequence Alignment

ATGCCTTATTTTACAAGACTCATTTTGTTCTTGTTTTGCTTGATGGTTCTCGTGGAGAGC
AGAAAACCCAAGAGGAAGCGATGGACAGGGCAGGTGGAAATGCCCAAGCCAAGTCACTTA
TATAAGAAGAACCTTGATGTGACCAAGATCCGGAAGGGAAAGCCTCAGCAGCTTCTCAGA
GTGGACGAGCACGACTTCAGCATGAGACCCGCCTTCGGAGGCCCTGCCATCCCGGTGGGC
GTGGACGTACAGGTGGAGAGCCTGGACAGCATCTCCGAGGTGGACATGGACTTCACTATG
ACCCTGTACCTGCGGCATTACTGGAAGGATGAGAGGCTAGCTTTCTCCAGCGCCAGCAAC
AAGAGCATGACCTTCGATGGCCGGCTGGTGAAGAAGATCTGGGTCCCTGATGTCTTCTTT
GTTCACTCCAAAAGATCGTTCACTCATGACACCACCACTGACAACATCATGCTGAGGGTG
TTCCCAGATGGACACGTGCTGTACAGCATGAGGATTACGGTCACTGCCATGTGCAACATG
GACTTCAGCCACTTTCCCCTGGACTCCCAGACCTGTTCTTTGGAGCTGGAGAGCTATGCC
TATACAGATGAAGATCTAATGCTGTACTGGAAGAATGGGGATGAATCCCTAAAAACAGAT
GAGAAGATCTCCTTGTCTCAGTTTCTGATTCAGAAATTTCACACAACTTCCAGGCTGGCC
TTCTACAGCAGCACTGGCTGGTACAACCGTCTGTACATTAACTTCACGTTGCGTCGCCAC
ATCTTCTTCTTCTTGCTCCAAACATATTTCCCTGCCACTCTGATGGTCATGCTGTCCTGG
GTGTCCTTCTGGATCGACCGCAGAGCTGTGCCTGCCAGAGTTTCACTGGGTATCACGACG
GTGCTGACCATGACCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTAC
GTCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTG
GAGTATGCGGCTGTCAACTACCTGACCACCGTGCAGGAGCGCAAGGAACGGAAGCTGCGG
GAGAAGTTCCCGTGCATGTGTGGAATGCTTCATTCAAAAACCATGATGCTGGATGGAAGC
TACAGTGAGTCTGAGGCCAACAGCCTGGCTGGGTACCCCAGAAGCCATATCCTGACAGAA
GAAGAAAGGCAAGACAAAATAGTGGTCCACCTGGGCCTGAGTGGTGAAGCCAACGCTGCC
AGAAAGAAGGGGCTTCTGAAGGGCCAGACGGGTTTTCGTATCTTCCAGAATACCCATGCC
ATTGACAAATACTCTAGGTTGATATTCCCTGCCTCCTACATATTTTTCAACTTAATTTAT
TGGTCAGTGTTTTCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GABRR2
Symbol Nomenclature : GABRR2
Designation : GABA(A) receptor subunit rho-2|GABA(A) receptor, rho 2|GABA(C) receptor|GABA-C receptor, rho-2 subunit|gamma-aminobutyric acid (GABA) receptor, rho 2|gamma-aminobutyric acid receptor subunit rho-2
Full Nomenclature : gamma-aminobutyric acid (GABA) A receptor, rho 2
GENEID : 2570
Locus Tag : RP1-131H7.1
GenBank Accession : 571768
HGNC : 4091
MIM : 137162
Vega : OTTHUMG00000015198
Target GenBank: HQ258439

 

Reference Sequence Alignment

  

HsCD00353766      ATGCCTTATTTTACAAGACTCATTTTGTTCTTGTTTTGCTTGATGGTTCTCGTGGAGAGC
NM_002043.3       ATGCCTTATTTTACAAGACTCATTTTGTTCTTGTTTTGCTTGATGGTTCTCGTGGAGAGC
                  ************************************************************

HsCD00353766      AGAAAACCCAAGAGGAAGCGATGGACAGGGCAGGTGGAAATGCCCAAGCCAAGTCACTTA
NM_002043.3       AGAAAACCCAAGAGGAAGCGATGGACAGGGCAGGTGGAAATGCCCAAGCCAAGTCACTTA
                  ************************************************************

HsCD00353766      TATAAGAAGAACCTTGATGTGACCAAGATCCGGAAGGGAAAGCCTCAGCAGCTTCTCAGA
NM_002043.3       TATAAGAAGAACCTTGATGTGACCAAGATCCGGAAGGGAAAGCCTCAGCAGCTTCTCAGA
                  ************************************************************

HsCD00353766      GTGGACGAGCACGACTTCAGCATGAGACCCGCCTTCGGAGGCCCTGCCATCCCGGTGGGC
NM_002043.3       GTGGACGAGCACGACTTCAGCATGAGACCCGCCTTCGGAGGCCCTGCCATCCCGGTGGGC
                  ************************************************************

HsCD00353766      GTGGACGTACAGGTGGAGAGCCTGGACAGCATCTCCGAGGTGGACATGGACTTCACTATG
NM_002043.3       GTGGACGTACAGGTGGAGAGCCTGGACAGCATCTCCGAGGTGGACATGGACTTCACTATG
                  ************************************************************

HsCD00353766      ACCCTGTACCTGCGGCATTACTGGAAGGATGAGAGGCTAGCTTTCTCCAGCGCCAGCAAC
NM_002043.3       ACCCTGTACCTGCGGCATTACTGGAAGGATGAGAGGCTAGCTTTCTCCAGCGCCAGCAAC
                  ************************************************************

HsCD00353766      AAGAGCATGACCTTCGATGGCCGGCTGGTGAAGAAGATCTGGGTCCCTGATGTCTTCTTT
NM_002043.3       AAGAGCATGACCTTCGATGGCCGGCTGGTGAAGAAGATCTGGGTCCCTGATGTCTTCTTT
                  ************************************************************

HsCD00353766      GTTCACTCCAAAAGATCGTTCACTCATGACACCACCACTGACAACATCATGCTGAGGGTG
NM_002043.3       GTTCACTCCAAAAGATCGTTCACTCATGACACCACCACTGACAACATCATGCTGAGGGTG
                  ************************************************************

HsCD00353766      TTCCCAGATGGACACGTGCTGTACAGCATGAGGATTACGGTCACTGCCATGTGCAACATG
NM_002043.3       TTCCCAGATGGACACGTGCTGTACAGCATGAGGATTACGGTCACTGCCATGTGCAACATG
                  ************************************************************

HsCD00353766      GACTTCAGCCACTTTCCCCTGGACTCCCAGACCTGTTCTTTGGAGCTGGAGAGCTATGCC
NM_002043.3       GACTTCAGCCACTTTCCCCTGGACTCCCAGACCTGTTCTTTGGAGCTGGAGAGCTATGCC
                  ************************************************************

HsCD00353766      TATACAGATGAAGATCTAATGCTGTACTGGAAGAATGGGGATGAATCCCTAAAAACAGAT
NM_002043.3       TATACAGATGAAGATCTAATGCTGTACTGGAAGAATGGGGATGAATCCCTAAAAACAGAT
                  ************************************************************

HsCD00353766      GAGAAGATCTCCTTGTCTCAGTTTCTGATTCAGAAATTTCACACAACTTCCAGGCTGGCC
NM_002043.3       GAGAAGATCTCCTTGTCTCAGTTTCTGATTCAGAAATTTCACACAACTTCCAGGCTGGCC
                  ************************************************************

HsCD00353766      TTCTACAGCAGCACTGGCTGGTACAACCGTCTGTACATTAACTTCACGTTGCGTCGCCAC
NM_002043.3       TTCTACAGCAGCACTGGCTGGTACAACCGTCTGTACATTAACTTCACGTTGCGTCGCCAC
                  ************************************************************

HsCD00353766      ATCTTCTTCTTCTTGCTCCAAACATATTTCCCTGCCACTCTGATGGTCATGCTGTCCTGG
NM_002043.3       ATCTTCTTCTTCTTGCTCCAAACATATTTCCCTGCCACTCTGATGGTCATGCTGTCCTGG
                  ************************************************************

HsCD00353766      GTGTCCTTCTGGATCGACCGCAGAGCTGTGCCTGCCAGAGTTTCACTGGGTATCACGACG
NM_002043.3       GTGTCCTTCTGGATCGACCGCAGAGCTGTGCCTGCCAGAGTTTCACTGGGTATCACGACG
                  ************************************************************

HsCD00353766      GTGCTGACCATGACCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTAC
NM_002043.3       GTGCTGACCATGACCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTAC
                  ************************************************************

HsCD00353766      GTCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTG
NM_002043.3       GTCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTG
                  ************************************************************

HsCD00353766      GAGTATGCGGCTGTCAACTACCTGACCACCGTGCAGGAGCGCAAGGAACGGAAGCTGCGG
NM_002043.3       GAGTATGCGGCTGTCAACTACCTGACCACCGTGCAGGAGCGCAAGGAACGGAAGCTGCGG
                  ************************************************************

HsCD00353766      GAGAAGTTCCCGTGCATGTGTGGAATGCTTCATTCAAAAACCATGATGCTGGATGGAAGC
NM_002043.3       GAGAAGTTCCCGTGCATGTGTGGAATGCTTCATTCAAAAACCATGATGCTGGATGGAAGC
                  ************************************************************

HsCD00353766      TACAGTGAGTCTGAGGCCAACAGCCTGGCTGGGTACCCCAGAAGCCATATCCTGACAGAA
NM_002043.3       TACAGTGAGTCTGAGGCCAACAGCCTGGCTGGGTACCCCAGAAGCCATATCCTGACAGAA
                  ************************************************************

HsCD00353766      GAAGAAAGGCAAGACAAAATAGTGGTCCACCTGGGCCTGAGTGGTGAAGCCAACGCTGCC
NM_002043.3       GAAGAAAGGCAAGACAAAATAGTGGTCCACCTGGGCCTGAGTGGTGAAGCCAACGCTGCC
                  ************************************************************

HsCD00353766      AGAAAGAAGGGGCTTCTGAAGGGCCAGACGGGTTTTCGTATCTTCCAGAATACCCATGCC
NM_002043.3       AGAAAGAAGGGGCTTCTGAAGGGCCAGACGGGTTTTCGTATCTTCCAGAATACCCATGCC
                  ************************************************************

HsCD00353766      ATTGACAAATACTCTAGGTTGATATTCCCTGCCTCCTACATATTTTTCAACTTAATTTAT
NM_002043.3       ATTGACAAATACTCTAGGTTGATATTCCCTGCCTCCTACATATTTTTCAACTTAATTTAT
                  ************************************************************

HsCD00353766      TGGTCAGTGTTTTCC---
NM_002043.3       TGGTCAGTGTTTTCCTAG
                  ***************   

Pathways

Reactome : GABA A (rho) receptor activation
Reactome : GABA receptor activation
Reactome : Ion channel transport
Reactome : Ligand-gated ion channel transport
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Transmembrane transport of small molecules
Reactome : Transmission across Chemical Synapses

Protein

UniProt : P28476
HPRD : 15922

Clone

Cloning Information : 571768

Organism


TAX_ID : 9606
Species Specific ID: 2570

Genome

Chromosome : 6
Map Location : 6q15
Ensembl : ENSG00000111886

Reagents

Labome : GABRR2-antibody