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AKTIP (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AKTIP
Gene Name:  AKT interacting protein
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100071412)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00356152

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 876nts         Open reading frame : 1 to 876
Reference Sequence Alignment

ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAG
AAGACATTAACAGGGGACGTGAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTG
CCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTACATCTCCTGCCCCAGCAGCA
CAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTAT
CGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGC
GTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTG
GTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTAT
GCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTA
CTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGC
ACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
TGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAA
GAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTA
CAGCCTTTCAGTAAAGAAGAGAAAACAGTGGCGACT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AKTIP
Symbol Nomenclature : AKTIP
SYNONYM : FT1; FTS
Designation : AKT-interacting protein|fused toes homolog|fused toes protein homolog
Full Nomenclature : AKT interacting protein
GENEID : 64400
GenBank Accession : 567514
HGNC : 16710
MIM : 608483
Vega : OTTHUMG00000133199
Target GenBank: HQ448037

 

Reference Sequence Alignment

  

HsCD00356152        ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAG
NM_022476.2         ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAG
NM_001012398.1      ATGAACCCTTTCTGGAGCATGTCTACAAGCTCTGTACGCAAACGATCTGAAGGTGAAGAG
                    ************************************************************

HsCD00356152        AAGACATTAACAGGGGACGTGAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTG
NM_022476.2         AAGACATTAACAGGGGACGTGAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTG
NM_001012398.1      AAGACATTAACAGGGGACGTGAAAACCAGTCCTCCACGAACTGCACCAAAGAAACAGCTG
                    ************************************************************

HsCD00356152        CCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTACATCTCCTGCCCCAGCAGCA
NM_022476.2         CCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTACATCTCCTGCCCCAGCAGCA
NM_001012398.1      CCTTCTATTCCCAAAAATGCTTTGCCCATAACTAAGCCTACATCTCCTGCCCCAGCAGCA
                    ************************************************************

HsCD00356152        CAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
NM_022476.2         CAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
NM_001012398.1      CAGTCAACAAATGGCACGCATGCGTCCTATGGACCCTTCTACCTGGAATACTCTCTTCTT
                    ************************************************************

HsCD00356152        GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTAT
NM_022476.2         GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTAT
NM_001012398.1      GCAGAATTTACCTTGGTTGTGAAGCAGAAGCTACCAGGCGTCTATGTGCAGCCATCTTAT
                    ************************************************************

HsCD00356152        CGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGC
NM_022476.2         CGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGC
NM_001012398.1      CGCTCTGCATTAATGTGGTTTGGAGTAATATTCATACGGCATGGACTTTACCAAGATGGC
                    ************************************************************

HsCD00356152        GTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTG
NM_022476.2         GTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTG
NM_001012398.1      GTATTTAAGTTTACAGTTTACATCCCTGATAACTATCCAGATGGTGACTGTCCACGCTTG
                    ************************************************************

HsCD00356152        GTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
NM_022476.2         GTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
NM_001012398.1      GTGTTCGATATTCCTGTCTTTCACCCGCTAGTTGATCCCACCTCAGGTGAGCTGGATGTG
                    ************************************************************

HsCD00356152        AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTAT
NM_022476.2         AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTAT
NM_001012398.1      AAGAGAGCATTTGCAAAATGGAGGCGGAACCATAATCATATTTGGCAGGTATTAATGTAT
                    ************************************************************

HsCD00356152        GCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTA
NM_022476.2         GCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTA
NM_001012398.1      GCAAGGAGAGTTTTCTACAAGATTGATACAGCAAGCCCCCTGAACCCAGAGGCTGCAGTA
                    ************************************************************

HsCD00356152        CTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGC
NM_022476.2         CTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGC
NM_001012398.1      CTGTATGAAAAAGATATTCAGCTTTTTAAAAGTAAAGTTGTTGACAGTGTTAAGGTGTGC
                    ************************************************************

HsCD00356152        ACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
NM_022476.2         ACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
NM_001012398.1      ACTGCTCGTTTGTTTGACCAACCTAAAATAGAAGACCCCTATGCAATTAGCTTTTCTCCA
                    ************************************************************

HsCD00356152        TGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAA
NM_022476.2         TGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAA
NM_001012398.1      TGGAATCCTTCTGTACATGATGAAGCCAGAGAAAAGATGCTGACTCAGAAAAAGCCTGAA
                    ************************************************************

HsCD00356152        GAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTA
NM_022476.2         GAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTA
NM_001012398.1      GAACAGCACAATAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTA
                    ************************************************************

HsCD00356152        CAGCCTTTCAGTAAAGAAGAGAAAACAGTGGCGACT---
NM_022476.2         CAGCCTTTCAGTAAAGAAGAGAAAACAGTGGCGACTTAA
NM_001012398.1      CAGCCTTTCAGTAAAGAAGAGAAAACAGTGGCGACTTAA
                    ************************************   

Clone

Cloning Information : 567514

Organism


TAX_ID : 9606
Species Specific ID: 64400

Genome

Chromosome : 16
Map Location : 16q12.2
Ensembl : ENSG00000166971

Reagents

Labome : AKTIP-antibody