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MTRF1L (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MTRF1L
Gene Name:  mitochondrial translational release factor 1-like
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100071230)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00356359

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1140nts         Open reading frame : 1 to 1140
Reference Sequence Alignment

ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCGCCCGGGGC
GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCAGGAGCTGCGGGAG
ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
GTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTA
GAAGAAGAAATAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGA
TCAGAGAAAATAAGAACATATAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAAC
AAGACGCTGCATGATCTTGAAACTTTTATGCAAGGAGATTATCTACTGGATGAACTTGTA
CAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAGAAATTATTTCCCAAAAAGTT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MTRF1L
Symbol Nomenclature : MTRF1L
SYNONYM : HMRF1L; MRF1L; mtRF1a
Designation : mitochondrial release factor 1 like|peptide chain release factor 1-like, mitochondrial
Full Nomenclature : mitochondrial translational release factor 1-like
GENEID : 54516
Locus Tag : RP1-101K10.1
GenBank Accession : 567756
HGNC : 21051
MIM : 613542
Vega : OTTHUMG00000015857
Target GenBank: HQ447880

 

Reference Sequence Alignment

  

HsCD00356359      ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
NM_019041.5       ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
                  ************************************************************

HsCD00356359      CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCGCCCGGGGC
NM_019041.5       CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCACCCGGGGC
                  ***************************************************.********

HsCD00356359      GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
NM_019041.5       GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
                  ************************************************************

HsCD00356359      AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCAGGAGCTGCGGGAG
NM_019041.5       AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCGGGAGCTGCGGGAG
                  **********************************************.*************

HsCD00356359      ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
NM_019041.5       ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
                  ************************************************************

HsCD00356359      ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
NM_019041.5       ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
                  ************************************************************

HsCD00356359      TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
NM_019041.5       TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
                  ************************************************************

HsCD00356359      GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
NM_019041.5       GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
                  ************************************************************

HsCD00356359      AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
NM_019041.5       AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
                  ************************************************************

HsCD00356359      TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
NM_019041.5       TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
                  ************************************************************

HsCD00356359      AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
NM_019041.5       AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
                  ************************************************************

HsCD00356359      GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
NM_019041.5       GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
                  ************************************************************

HsCD00356359      ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
NM_019041.5       ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
                  ************************************************************

HsCD00356359      GTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
NM_019041.5       GTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
                  ************************************************************

HsCD00356359      CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTA
NM_019041.5       CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTA
                  ************************************************************

HsCD00356359      GAAGAAGAAATAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGA
NM_019041.5       GAAGAAGAAATAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGA
                  ************************************************************

HsCD00356359      TCAGAGAAAATAAGAACATATAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAAC
NM_019041.5       TCAGAGAAAATAAGAACATATAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAAC
                  ************************************************************

HsCD00356359      AAGACGCTGCATGATCTTGAAACTTTTATGCAAGGAGATTATCTACTGGATGAACTTGTA
NM_019041.5       AAGACGCTGCATGATCTTGAAACTTTTATGCAAGGAGATTATCTACTGGATGAACTTGTA
                  ************************************************************

HsCD00356359      CAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAGAAATTATTTCCCAAAAAGTT
NM_019041.5       CAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAGAAATTATTTCCCAAAAAGTT
                  ************************************************************

HsCD00356359      ---
NM_019041.5       TAA
                     

Protein

SMART domain : PCRF :
UniProt : Q9UGC7
HPRD : 17613

Clone

Cloning Information : 567756

Organism


TAX_ID : 9606
Species Specific ID: 54516

Genome

Chromosome : 6
Map Location : 6q25-q26
Ensembl : ENSG00000112031

Reagents

Labome : MTRF1L-antibody