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MRI1 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MRI1
Gene Name:  methylthioribose-1-phosphate isomerase 1
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100071642)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00357468

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1107nts         Open reading frame : 1 to 1107
Reference Sequence Alignment

ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
GATGGAACCCTAGATGGACCCCAGATG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MRI1
Symbol Nomenclature : MRI1
SYNONYM : MRDI; MTNA; Ypr118w
Designation : M1Pi|MTR-1-P isomerase|S-methyl-5-thioribose-1-phosphate isomerase 1|mediator of RhoA-dependent invasion|methylthioribose-1-phosphate isomerase|methylthioribose-1-phosphate isomerase homolog|translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Full Nomenclature : methylthioribose-1-phosphate isomerase 1
GENEID : 84245
Locus Tag : UNQ6390/PRO21135
GenBank Accession : 569751
HGNC : 28469
MIM : 615105
Vega : OTTHUMG00000180867
Target GenBank: HQ448241

 

Reference Sequence Alignment

  

HsCD00357468        ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
NM_001031727.2      ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
                    ************************************************************

HsCD00357468        CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
NM_001031727.2      CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
                    ************************************************************

HsCD00357468        CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
NM_001031727.2      CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
                    ************************************************************

HsCD00357468        GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
NM_001031727.2      GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
                    ************************************************************

HsCD00357468        GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
NM_001031727.2      GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
                    ************************************************************

HsCD00357468        CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
NM_001031727.2      CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
                    ************************************************************

HsCD00357468        GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
NM_001031727.2      GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
                    ************************************************************

HsCD00357468        CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
NM_001031727.2      CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
                    ************************************************************

HsCD00357468        AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
NM_001031727.2      AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
                    ************************************************************

HsCD00357468        GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
NM_001031727.2      GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
                    ************************************************************

HsCD00357468        GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
NM_001031727.2      GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
                    ************************************************************

HsCD00357468        ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
NM_001031727.2      ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
                    ************************************************************

HsCD00357468        TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
NM_001031727.2      TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
                    ************************************************************

HsCD00357468        GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
NM_001031727.2      GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
                    ************************************************************

HsCD00357468        CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
NM_001031727.2      CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
                    ************************************************************

HsCD00357468        CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
NM_001031727.2      CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
                    ************************************************************

HsCD00357468        TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
NM_001031727.2      TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
                    ************************************************************

HsCD00357468        CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
NM_001031727.2      CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
                    ************************************************************

HsCD00357468        GATGGAACCCTAGATGGACCCCAGATG---
NM_001031727.2      GATGGAACCCTAGATGGACCCCAGATGTAA
                    ***************************   

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Protein

UniProt : Q9BV20
HPRD : 14548

Clone

Cloning Information : 569751

Organism


TAX_ID : 9606
Species Specific ID: 84245

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000037757

Reagents

Labome : MRI1-antibody