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UBE2Q2 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  UBE2Q2
Gene Name:  ubiquitin-conjugating enzyme E2Q family member 2
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100071631)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00357474

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1125nts         Open reading frame : 1 to 1125
Reference Sequence Alignment

ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCCATCTTCGACAAGAAC
CACGAGCGATTCCGCATCGTCAGTTGGAAGCTGGACGAGCTGCACTGCCAGTTCCTGGTG
CCGCAGCAGGGCAGCCCGCACTCGCTGCCGCCGCCACTCACGCTCCACTGCAACATCACG
GAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACA
TCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAATTGAAG
TGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCACCTGGATGTTGAGATG
CTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAAGAAGTGACTTCAGAAGAA
GAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTAGATCACTATGAGATGAAG
GAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTG
GCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCT
GGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGACATATACAGATCACAG
AGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGACAGTTTATATGACTGGCAT
GTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAA
GAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTT
GATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTGGGTGGA
GGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAA
TCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAAGCCAGAGTGCAGTTTGGA
GCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCCTATAATTCCATTGTACAG
ATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGATGGC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2Q2
Symbol Nomenclature : UBE2Q2
Designation : ubiquitin carrier protein Q2|ubiquitin-conjugating enzyme E2 Q2|ubiquitin-protein ligase Q2
Full Nomenclature : ubiquitin-conjugating enzyme E2Q family member 2
GENEID : 92912
GenBank Accession : 569758
HGNC : 19248
MIM : 612501
Vega : OTTHUMG00000142839
Target GenBank: HQ448230

 

Reference Sequence Alignment

  

HsCD00357474        ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCCATCTTCGACAAGAAC
NM_173469.3         ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCCATCTTCGACAAGAAC
NM_001145335.1      ------------------------------------ATGAGAATGGACTCGTTGACGGAG
NM_001284382.1      ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCCATCTTCGACAAGAAC
                                                        .**. .:  .:**   : *.*.* 

HsCD00357474        CACGAGCGATTCCGCATCGTCAGTTGGAAGCTGGACGAGCTGCACTGCCAGTTCCTGGTG
NM_173469.3         CACGAGCGATTCCGCATCGTCAGTTGGAAGCTGGACGAGCTGCACTGCCAGTTCCTGGTG
NM_001145335.1      GAAAAGTTGGAATGCAGACTCTGGTGCTGTTTGAGCGACCCCTCTCCCCCGGGCCTGGCT
NM_001284382.1      CACGAGCGATTCCGCATCGTCAGTTGGAAGCTGGACGAGCTGCACTGCCAGTTCCTGGTG
                     *..**  . :. *** . **:* ** :.  **..*** *   .   **.*  *****  

HsCD00357474        CCGCAGCAGGGCAGCCCGCACTCGCTGCCGCCGCCACTCACGCTCCACTGCAACATCACG
NM_173469.3         CCGCAGCAGGGCAGCCCGCACTCGCTGCCGCCGCCACTCACGCTCCACTGCAACATCACG
NM_001145335.1      GCGCGCTG------------CTGTGTTCTGGAAAGGAGCATAGTACCGTCGTTGCGGCAG
NM_001284382.1      CCGCAGCAGGGCAGCCCGCACTCGCTGCCGCCGCCACTCACGCTCCACTGCAACATCACG
                     ***.  .            **   * * * ... .. ** . *.*. *  :: .  ..*

HsCD00357474        GAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACA
NM_173469.3         GAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACA
NM_001145335.1      GAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACA
NM_001284382.1      GAATCCTATCCATCTTCTTCACCGATATGGTTTGTGGATTCTGAAGACCCAAATCTGACA
                    ************************************************************

HsCD00357474        TCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAATTGAAG
NM_173469.3         TCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAATTGAAG
NM_001145335.1      TCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGCTTCGTCAGCAATTGAAG
NM_001284382.1      TCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATTTGAATG--------------
                    ******************************************.:*               

HsCD00357474        TGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCACCTGGATGTTGAGATG
NM_173469.3         TGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCACCTGGATGTTGAGATG
NM_001145335.1      TGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCACCTGGATGTTGAGATG
NM_001284382.1      ------------------------------------------------------------
                                                                                

HsCD00357474        CTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAAGAAGTGACTTCAGAAGAA
NM_173469.3         CTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAAGAAGTGACTTCAGAAGAA
NM_001145335.1      CTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAAGAAGTGACTTCAGAAGAA
NM_001284382.1      -------------------------------GGACAACAGAAGAAGTGACTTCAGAAGAA
                                                   *****************************

HsCD00357474        GAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTAGATCACTATGAGATGAAG
NM_173469.3         GAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTAGATCACTATGAGATGAAG
NM_001145335.1      GAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTAGATCACTATGAGATGAAG
NM_001284382.1      GAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTAGATCACTATGAGATGAAG
                    ************************************************************

HsCD00357474        GAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTG
NM_173469.3         GAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTG
NM_001145335.1      GAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTG
NM_001284382.1      GAAGAAGAGCCTATTAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTG
                    ************************************************************

HsCD00357474        GCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCT
NM_173469.3         GCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCT
NM_001145335.1      GCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCT
NM_001284382.1      GCAATATTAGAGAAAATTAGGAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCT
                    ************************************************************

HsCD00357474        GGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGACATATACAGATCACAG
NM_173469.3         GGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGACATATACAGATCACAG
NM_001145335.1      GGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGACATATACAGATCACAG
NM_001284382.1      GGGTCAGTGCAAGCTTCAGATAGACTTATGAAAGAGCTCAGGGACATATACAGATCACAG
                    ************************************************************

HsCD00357474        AGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGACAGTTTATATGACTGGCAT
NM_173469.3         AGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGACAGTTTATATGACTGGCAT
NM_001145335.1      AGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGACAGTTTATATGACTGGCAT
NM_001284382.1      AGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATGACAGTTTATATGACTGGCAT
                    ************************************************************

HsCD00357474        GTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAA
NM_173469.3         GTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAA
NM_001145335.1      GTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAA
NM_001284382.1      GTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGTGATCTTCAGATCTTAAAA
                    ************************************************************

HsCD00357474        GAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTT
NM_173469.3         GAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTT
NM_001145335.1      GAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTT
NM_001284382.1      GAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTT
                    ************************************************************

HsCD00357474        GATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTGGGTGGA
NM_173469.3         GATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTGGGTGGA
NM_001145335.1      GATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTGGGTGGA
NM_001284382.1      GATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTGGGTGGA
                    ************************************************************

HsCD00357474        GGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAA
NM_173469.3         GGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAA
NM_001145335.1      GGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAA
NM_001284382.1      GGAGCATTATGTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAA
                    ************************************************************

HsCD00357474        TCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAAGCCAGAGTGCAGTTTGGA
NM_173469.3         TCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAAGCCAGAGTGCAGTTTGGA
NM_001145335.1      TCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAAGCCAGAGTGCAGTTTGGA
NM_001284382.1      TCGGTCATCATGCAAATAAATGCCACCTTAGTCAAAGGCAAAGCCAGAGTGCAGTTTGGA
                    ************************************************************

HsCD00357474        GCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCCTATAATTCCATTGTACAG
NM_173469.3         GCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCCTATAATTCCATTGTACAG
NM_001145335.1      GCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCCTATAATTCCATTGTACAG
NM_001284382.1      GCAAATAAGAATCAATATAATCTAGCAAGAGCCCAACAATCCTATAATTCCATTGTACAG
                    ************************************************************

HsCD00357474        ATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGATGGC---
NM_173469.3         ATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGATGGCTAA
NM_001145335.1      ATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGATGGCTAA
NM_001284382.1      ATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAGATGGCTAA
                    *********************************************   

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : UBCc : Ubiquitin-conjugating enzyme E2, catalytic domain homologues
UniProt : B7Z3Q2
UniProt : Q8WVN8
UniProt : E9PHD0
HPRD : 14294

Clone

Cloning Information : 569758

Organism


TAX_ID : 9606
Species Specific ID: 92912

Genome

Chromosome : 15
Map Location : 15q24.2
Ensembl : ENSG00000140367

Reagents

Labome : UBE2Q2-antibody