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GCNT4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GCNT4
Gene Name:  glucosaminyl (N-acetyl) transferase 4, core 2
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358268

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1260nts         Open reading frame : 1 to 1260
Reference Sequence Alignment

AGACGACTCTTTCCGCAAAAAGACATTTACTTGGTTGAGTACTCCCTAAGTACCTCGCCT
TTTGTAAGAAACAGATACACTCATGTTAAGGATGAAGTCAGGTATGAAGTTAACTGTTCG
GGTATCTATGAACAGGAGCCTTTGGAAATTGGAAAGAGTCTGGAAATAAGAAGAAGGGAC
ATCATTGACTTGGAGGATGATGATGTTGTGGCAATGACCAGTGATTGTGACATTTATCAG
ACTCTAAGAGGTTATGCTCAAAAGCTTGTCTCAAAGGAGGAGAAAAGCTTCCCAATAGCC
TATTCTTTGGTTGTCCACAAAGATGCAATTATGGTTGAAAGGCTTATCCATGCTATATAC
AACCAGCACAATATTTACTGCATCCATTATGATCGTAAGGCACCTGATACCTTCAAAGTT
GCCATGAACAATTTAGCTAAGTGCTTCTCCAATATTTTCATTGCTTCCAAATTAGAGGCT
GTGGAATATGCCCACATTTCCAGACTCCAGGCTGATTTAAATTGCTTGTCGGACCTTCTG
AAGTCTTCAATCCAGTGGAAATATGTTATCAACTTGTGTGGGCAAGATTTTCCCCTGAAG
TCAAATTTTGAATTGGTGTCAGAGTTGAAAAAACTCAATGGAGCAAATATGTTGGAGACG
GTGAAACCCCCAAACAGTAAATTGGAAAGATTCACTTACCATCATGAACTTAGACGGGTG
CCTTATGAATATGTGAAGCTACCAATAAGGACAAACATCTCCAAGGAAGCACCCCCCCAT
AACATTCAGATATTTGTTGGCAGTGCTTATTTTGTTTTAAGTCAAGCATTTGTTAAATAT
ATTTTCAACAACTCCATCGTTCAAGACTTTTTTGCCTGGTCTAAAGACACATACTCTCCT
GATGAGCACTTTTGGGCTACCTTGATTCGGGTTCCAGGAATACCTGGGGAGATTTCCAGA
TCAGCCCAGGATGTGTCTGATCTGCAGAGTAAGACTCGCCTTGTCAAGTGGAATTACTAT
GAAGGCTTTTTCTATCCCAGTTGTACTGGATCTCACCTTCGAAGCGTGTGTATTTATGGA
GCTGCAGAATTAAGGTGGCTTATCAAAGATGGACATTGGTTTGCTAATAAATTTGATTCT
AAGGTGGACCCTATCTTGATTAAATGCTTGGCAGAAAAGCTTGAAGAACAGCAGAGAGAC
TGGATCACTTTGCCCTCAGAAAAGTTATTTATGGATAGAAATCTCACTACCACATCATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,102

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GCNT4
Symbol Nomenclature : GCNT4
SYNONYM : C2GNT3
Designation : beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4|core 2 beta-1,6-N-acetylglucosaminyltransferase 3|core 2-branching enzyme 3|core2-GlcNAc-transferase 3|glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
Full Nomenclature : glucosaminyl (N-acetyl) transferase 4, core 2
GENEID : 51301
GenBank Accession : BC160070
HGNC : 17973
Vega : OTTHUMG00000131272
Target GenBank: BC160070

 

Reference Sequence Alignment

  

HsCD00358268      ------------------------------------------------------------
NM_016591.2       ATGAAGATATTCAAATGTTATTTTAAACATACCCTACAGCAGAAAGTTTTCATCCTGTTT
                                                                              

HsCD00358268      ------------------------------------------AGACGACTCTTTCCGCAA
NM_016591.2       TTAACCCTATGGCTGCTCTCTTTGTTAAAGCTTCTAAATGTGAGACGACTCTTTCCGCAA
                                                            ******************

HsCD00358268      AAAGACATTTACTTGGTTGAGTACTCCCTAAGTACCTCGCCTTTTGTAAGAAACAGATAC
NM_016591.2       AAAGACATTTACTTGGTTGAGTACTCCCTAAGTACCTCGCCTTTTGTAAGAAACAGATAC
                  ************************************************************

HsCD00358268      ACTCATGTTAAGGATGAAGTCAGGTATGAAGTTAACTGTTCGGGTATCTATGAACAGGAG
NM_016591.2       ACTCATGTTAAGGATGAAGTCAGGTATGAAGTTAACTGTTCGGGTATCTATGAACAGGAG
                  ************************************************************

HsCD00358268      CCTTTGGAAATTGGAAAGAGTCTGGAAATAAGAAGAAGGGACATCATTGACTTGGAGGAT
NM_016591.2       CCTTTGGAAATTGGAAAGAGTCTGGAAATAAGAAGAAGGGACATCATTGACTTGGAGGAT
                  ************************************************************

HsCD00358268      GATGATGTTGTGGCAATGACCAGTGATTGTGACATTTATCAGACTCTAAGAGGTTATGCT
NM_016591.2       GATGATGTTGTGGCAATGACCAGTGATTGTGACATTTATCAGACTCTAAGAGGTTATGCT
                  ************************************************************

HsCD00358268      CAAAAGCTTGTCTCAAAGGAGGAGAAAAGCTTCCCAATAGCCTATTCTTTGGTTGTCCAC
NM_016591.2       CAAAAGCTTGTCTCAAAGGAGGAGAAAAGCTTCCCAATAGCCTATTCTTTGGTTGTCCAC
                  ************************************************************

HsCD00358268      AAAGATGCAATTATGGTTGAAAGGCTTATCCATGCTATATACAACCAGCACAATATTTAC
NM_016591.2       AAAGATGCAATTATGGTTGAAAGGCTTATCCATGCTATATACAACCAGCACAATATTTAC
                  ************************************************************

HsCD00358268      TGCATCCATTATGATCGTAAGGCACCTGATACCTTCAAAGTTGCCATGAACAATTTAGCT
NM_016591.2       TGCATCCATTATGATCGTAAGGCACCTGATACCTTCAAAGTTGCCATGAACAATTTAGCT
                  ************************************************************

HsCD00358268      AAGTGCTTCTCCAATATTTTCATTGCTTCCAAATTAGAGGCTGTGGAATATGCCCACATT
NM_016591.2       AAGTGCTTCTCCAATATTTTCATTGCTTCCAAATTAGAGGCTGTGGAATATGCCCACATT
                  ************************************************************

HsCD00358268      TCCAGACTCCAGGCTGATTTAAATTGCTTGTCGGACCTTCTGAAGTCTTCAATCCAGTGG
NM_016591.2       TCCAGACTCCAGGCTGATTTAAATTGCTTGTCGGACCTTCTGAAGTCTTCAATCCAGTGG
                  ************************************************************

HsCD00358268      AAATATGTTATCAACTTGTGTGGGCAAGATTTTCCCCTGAAGTCAAATTTTGAATTGGTG
NM_016591.2       AAATATGTTATCAACTTGTGTGGGCAAGATTTTCCCCTGAAGTCAAATTTTGAATTGGTG
                  ************************************************************

HsCD00358268      TCAGAGTTGAAAAAACTCAATGGAGCAAATATGTTGGAGACGGTGAAACCCCCAAACAGT
NM_016591.2       TCAGAGTTGAAAAAACTCAATGGAGCAAATATGTTGGAGACGGTGAAACCCCCAAACAGT
                  ************************************************************

HsCD00358268      AAATTGGAAAGATTCACTTACCATCATGAACTTAGACGGGTGCCTTATGAATATGTGAAG
NM_016591.2       AAATTGGAAAGATTCACTTACCATCATGAACTTAGACGGGTGCCTTATGAATATGTGAAG
                  ************************************************************

HsCD00358268      CTACCAATAAGGACAAACATCTCCAAGGAAGCACCCCCCCATAACATTCAGATATTTGTT
NM_016591.2       CTACCAATAAGGACAAACATCTCCAAGGAAGCACCCCCCCATAACATTCAGATATTTGTT
                  ************************************************************

HsCD00358268      GGCAGTGCTTATTTTGTTTTAAGTCAAGCATTTGTTAAATATATTTTCAACAACTCCATC
NM_016591.2       GGCAGTGCTTATTTTGTTTTAAGTCAAGCATTTGTTAAATATATTTTCAACAACTCCATC
                  ************************************************************

HsCD00358268      GTTCAAGACTTTTTTGCCTGGTCTAAAGACACATACTCTCCTGATGAGCACTTTTGGGCT
NM_016591.2       GTTCAAGACTTTTTTGCCTGGTCTAAAGACACATACTCTCCTGATGAGCACTTTTGGGCT
                  ************************************************************

HsCD00358268      ACCTTGATTCGGGTTCCAGGAATACCTGGGGAGATTTCCAGATCAGCCCAGGATGTGTCT
NM_016591.2       ACCTTGATTCGGGTTCCAGGAATACCTGGGGAGATTTCCAGATCAGCCCAGGATGTGTCT
                  ************************************************************

HsCD00358268      GATCTGCAGAGTAAGACTCGCCTTGTCAAGTGGAATTACTATGAAGGCTTTTTCTATCCC
NM_016591.2       GATCTGCAGAGTAAGACTCGCCTTGTCAAGTGGAATTACTATGAAGGCTTTTTCTATCCC
                  ************************************************************

HsCD00358268      AGTTGTACTGGATCTCACCTTCGAAGCGTGTGTATTTATGGAGCTGCAGAATTAAGGTGG
NM_016591.2       AGTTGTACTGGATCTCACCTTCGAAGCGTGTGTATTTATGGAGCTGCAGAATTAAGGTGG
                  ************************************************************

HsCD00358268      CTTATCAAAGATGGACATTGGTTTGCTAATAAATTTGATTCTAAGGTGGACCCTATCTTG
NM_016591.2       CTTATCAAAGATGGACATTGGTTTGCTAATAAATTTGATTCTAAGGTGGACCCTATCTTG
                  ************************************************************

HsCD00358268      ATTAAATGCTTGGCAGAAAAGCTTGAAGAACAGCAGAGAGACTGGATCACTTTGCCCTCA
NM_016591.2       ATTAAATGCTTGGCAGAAAAGCTTGAAGAACAGCAGAGAGACTGGATCACTTTGCCCTCA
                  ************************************************************

HsCD00358268      GAAAAGTTATTTATGGATAGAAATCTCACTACCACATCATAG
NM_016591.2       GAAAAGTTATTTATGGATAGAAATCTCACTACCACATCATGA
                  ****************************************..

Pathways

Reactome : Metabolism of proteins
Reactome : O-linked glycosylation
Reactome : O-linked glycosylation of mucins
Reactome : Post-translational protein modification

Protein

UniProt : Q9P109
HPRD : 16651

Clone

Cloning Information : 560402

Organism


TAX_ID : 9606
Species Specific ID: 51301

Genome

Chromosome : 5
Map Location : 5q12
Ensembl : ENSG00000176928

Reagents

Labome : GCNT4-antibody