DNASU Plasmid Repository • 480.965.5697 | Email

MBP (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  MBP
Gene Name:  myelin basic protein
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH016242.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00003714

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 516nts         Open reading frame : 1 to 516
Reference Sequence Alignment

ATGGCGTCACAGAAGAGACCCTCCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGT
ACCATGGACCATGCCAGGCATGGCTTCCTCCCAAGGCACAGAGACACGGGCATCCTTGAC
TCCATCGGGCGCTTCTTTGGCGGTGACAGGGGTGCGCCCAAGCGGGGCTCTGGCAAGGAC
TCACACCACCCGGCAAGAACTGCTCACTACGGCTCCCTGCCCCAGAAGTCACACGGCCGG
ACCCAAGATGAAAACCCCGTAGTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCA
CCCCCGTCGCAGGGAAAGGGGAGAGGACTGTCCCTGAGCAGATTTAGCTGGGGGGCCGAA
GGCCAGAGACCAGGATTTGGCTACGGAGGCAGAGCGTCCGACTATAAATCGGCTCACAAG
GGATTCAAGGGAGTCGATGCCCAGGGCACGCTTTCCAAAATTTTTAAGCTGGGAGGAAGA
GATAGTCGCTCTGGATCACCCATGGCTAGACGCTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 554
Start on reference sequence 39
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MBP
Symbol Nomenclature : MBP
Designation : myelin A1 protein|myelin membrane encephalitogenic protein
Full Nomenclature : myelin basic protein
GENEID : 4155
GI : 60656032
GenBank Accession : AY889789
HGNC : 6925
MIM : 159430
Vega : OTTHUMG00000132874
Target GenBank: BC008749

 

Reference Sequence Alignment

  

HsCD00003714        ------------------------------------------------------------
NM_001025092.1      ------------------------------------------------------------
NM_001025101.1      ATGGGAAACCACGCAGGCAAACGAGAATTAAATGCCGAGAAGGCCAGTACGAATAGTGAA
XM_005266699.1      ATGGGAAACCACGCAGGCAAACGAGAATTAAATGCCGAGAAGGCCAGTACGAATAGTGAA
NM_001025090.1      ------------------------------------------------------------
                                                                                

HsCD00003714        ------------------------------------------------------------
NM_001025092.1      ------------------------------------------------------------
NM_001025101.1      ACTAACAGAGGAGAATCTGAAAAAAAGAGAAACCTGGGTGAACTTTCACGGACAACCTCA
XM_005266699.1      ACTAACAGAGGAGAATCTGAAAAAAAGAGAAACCTGGGTGAACTTTCACGGACAACCTCA
NM_001025090.1      ------------------------------------------------------------
                                                                                

HsCD00003714        ------------------------------------------------------------
NM_001025092.1      ------------------------------------------------------------
NM_001025101.1      GAGGACAACGAAGTGTTCGGAGAGGCAGATGCGAACCAGAACAATGGGACCTCCTCTCAG
XM_005266699.1      GAGGACAACGAAGTGTTCGGAGAGGCAGATGCGAACCAGAACAATGGGACCTCCTCTCAG
NM_001025090.1      ------------------------------------------------------------
                                                                                

HsCD00003714        ------------------------------------------------------------
NM_001025092.1      ------------------------------------------------------------
NM_001025101.1      GACACAGCGGTGACTGACTCCAAGCGCACAGCGGACCCGAAGAATGCCTGGCAGGATGCC
XM_005266699.1      GACACAGCGGTGACTGACTCCAAGCGCACAGCGGACCCGAAGAATGCCTGGCAGGATGCC
NM_001025090.1      ------------------------------------------------------------
                                                                                

HsCD00003714        ------------------------------------------------------------
NM_001025092.1      ------------------------------------------------------------
NM_001025101.1      CACCCAGCTGACCCAGGGAGCCGCCCCCACTTGATCCGCCTCTTTTCCCGAGATGCCCCG
XM_005266699.1      CACCCAGCTGACCCAGGGAGCCGCCCCCACTTGATCCGCCTCTTTTCCCGAGATGCCCCG
NM_001025090.1      ------------------------------------------------------------
                                                                                

HsCD00003714        ------------------------------------------------------------
NM_001025092.1      ------------------------------------------------------------
NM_001025101.1      GGGAGGGAGGACAACACCTTCAAAGACAGGCCCTCTGAGTCCGACGAGCTCCAGACCATC
XM_005266699.1      GGGAGGGAGGACAACACCTTCAAAGACAGGCCCTCTGAGTCCGACGAGCTCCAGACCATC
NM_001025090.1      ------------------------------------------------------------
                                                                                

HsCD00003714        ---------------------------------------ATGGCGTCACAGAAGAGACCC
NM_001025092.1      ---------------------------------------ATGGCGTCACAGAAGAGACCC
NM_001025101.1      CAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGATGGCGTCACAGAAGAGACCC
XM_005266699.1      CAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGATGGCGTCACAGAAGAGACCC
NM_001025090.1      ---------------------------------------ATGGCGTCACAGAAGAGACCC
                                                           *********************

HsCD00003714        TCCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGTACCATGGACCATGCCAGGCAT
NM_001025092.1      TCCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGTACCATGGACCATGCCAGGCAT
NM_001025101.1      TCCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGTACCATGGACCATGCCAGGCAT
XM_005266699.1      TCCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGTACCATGGACCATGCCAGGCAT
NM_001025090.1      TCCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGTACCATGGACCATGCCAGGCAT
                    ************************************************************

HsCD00003714        GGCTTCCTCCCAAGGCACAGAGACACGGGCATCCTTGACTCCATCGGGCGCTTCTTTGGC
NM_001025092.1      GGCTTCCTCCCAAGGCACAGAGACACGGGCATCCTTGACTCCATCGGGCGCTTCTTTGGC
NM_001025101.1      GGCTTCCTCCCAAGGCACAGAGACACGGGCATCCTTGACTCCATCGGGCGCTTCTTTGGC
XM_005266699.1      GGCTTCCTCCCAAGGCACAGAGACACGGGCATCCTTGACTCCATCGGGCGCTTCTTTGGC
NM_001025090.1      GGCTTCCTCCCAAGGCACAGAGACACGGGCATCCTTGACTCCATCGGGCGCTTCTTTGGC
                    ************************************************************

HsCD00003714        GGTGACAGGGGTGCGCCCAAGCGGGGCTCTGGCAAGGACTCACACCACCCGGCAAGAACT
NM_001025092.1      GGTGACAGGGGTGCGCCCAAGCGGGGCTCTGGCAAGGACTCACACCACCCGGCAAGAACT
NM_001025101.1      GGTGACAGGGGTGCGCCCAAGCGGGGCTCTGGCAAGGACTCACACCACCCGGCAAGAACT
XM_005266699.1      GGTGACAGGGGTGCGCCCAAGCGGGGCTCTGGCAAGGACTCACACCACCCGGCAAGAACT
NM_001025090.1      GGTGACAGGGGTGCGCCCAAGCGGGGCTCTGGCAAGGACTCACACCACCCGGCAAGAACT
                    ************************************************************

HsCD00003714        GCTCACTACGGCTCCCTGCCCCAGAAGTCACACGGCCGGACCCAAGATGAAAACCCCGTA
NM_001025092.1      GCTCACTACGGCTCCCTGCCCCAGAAGTCACACGGCCGGACCCAAGATGAAAACCCCGTA
NM_001025101.1      GCTCACTACGGCTCCCTGCCCCAGAAGTCACACGGCCGGACCCAAGATGAAAACCCCGTA
XM_005266699.1      GCTCACTACGGCTCCCTGCCCCAGAAGTCACACGGCCGGACCCAAGATGAAAACCCCGTA
NM_001025090.1      GCTCACTACGGCTCCCTGCCCCAGAAGTCACACGGCCGGACCCAAGATGAAAACCCCGTA
                    ************************************************************

HsCD00003714        GTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCACCCCCGTCGCAGGGAAAGGGG
NM_001025092.1      GTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCACCCCCGTCGCAGGGAAAGGGG
NM_001025101.1      GTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCACCCCCGTCGCAGGGAAAGGGG
XM_005266699.1      GTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCACCCCCGTCGCAGGGAAAG---
NM_001025090.1      GTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCACCCCCGTCGCAGGGAAAGGGG
                    *********************************************************   

HsCD00003714        AGAGGACTGTCCCTGAGCAGATTTAGCTGGGGGGCCGAAGGCCAGAGACCAGGATTTGGC
NM_001025092.1      G---------------------------------CCGAAGGCCAGAGACCAGGATTTGGC
NM_001025101.1      AGAGGACTGTCCCTGAGCAGATTTAGCTGGGGGGCCGAAGGCCAGAGACCAGGATTTGGC
XM_005266699.1      ------------------------------GGGGCCGAAGGCCAGAGACCAGGATTTGGC
NM_001025090.1      AGAGGACTGTCCCTGAGCAGATTTAGCTGGGGGGCCGAAGGCCAGAGACCAGGATTTGGC
                                                      **************************

HsCD00003714        TACGGAGGCAGAGCGTCCGACTATAAATCGGCTCACAAGGGATTCAAGGGAGTCGATGCC
NM_001025092.1      TACGGAGGCAGAGCGTCCGACTATAAATCGGCTCACAAGGGATTCAAGGGAGTCGATGCC
NM_001025101.1      TACGGAGGCAGAGCGTCCGACTATAAATCGGCTCACAAGGGATTCAAGGGAGTCGATGCC
XM_005266699.1      TACGGAGGCAGAGCGTCCGACTATAAATCGGCTCACAAGGGATTCAAGGGAGTCGATGCC
NM_001025090.1      TACGGAGGCAGAGCGTCCGACTATAAATCGGCTCACAAGGGATTCAAGGGAGTCGATGCC
                    ************************************************************

HsCD00003714        CAGGGCACGCTTTCCAAAATTTTTAAGCTGGGAGGAAGAGATAGTCGCTCTGGATCACCC
NM_001025092.1      CAGGGCACGCTTTCCAAAATTTTTAAGCTGGGAGGAAGAGATAGTCGCTCTGGATCACCC
NM_001025101.1      CAGGGCACGCTTTCCAAAATTTTTAAGCTGGGAGGAAGAGATAGTCGCTCTGGATCACCC
XM_005266699.1      CAGGGCACGCTTTCCAAAATTTTTAAGCTGGGAGGAAGAGATAGTCGCTCTGGATCACCC
NM_001025090.1      CAGGGCACGCTTTCCAAAATTTTTAAGCTGGGAGGAAGAGATAGTCGCTCTGGATCACCC
                    ************************************************************

HsCD00003714        ATGGCTAGACGCTGA
NM_001025092.1      ATGGCTAGACGCTGA
NM_001025101.1      ATGGCTAGACGCTGA
XM_005266699.1      ATGGCTAGACGCTGA
NM_001025090.1      ATGGCTAGACGCTGA
                    ***************

Pathways

Reactome : Complement cascade
Reactome : Creation of C4 and C2 activators
Reactome : Immune System
Reactome : Initial triggering of complement
Reactome : Innate Immune System
Reactome : Lectin pathway of complement activation
WikiPathway : Regulation of toll-like receptor signaling pathway

Protein

UniProt : Q5SQS3
UniProt : P11226
HPRD : 01158

Clone

Cloning Information : 16242
HIP Master Clone ID : 30441
Original Clone ID : FLH016242.01X

Organism


TAX_ID : 9606
Species Specific ID: 4153

Genome

Chromosome : 18
Map Location : 18q23
Ensembl : ENSG00000197971

Reagents

Labome : MBP-antibody