Gene:
Gene Symbol:
TM0882
Gene Name:
O-acetylhomoserine sulfhydrylase
Original Clone ID:
8169
Keyword:
full-length cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00382426
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1293nts
Open reading frame: 51 to 1343
GAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCAATGGACTGGA
AGAAATACGGTTACAACACAAGGGCTCTTCACGCAGGTTATGAACCACCCGAGCAGGCCA
CAGGATCGAGAGCGGTCCCTATATATCAAACGACTTCTTACGTTTTCAGAGACTCTGATC
ACGCGGCGAGACTCTTCGCACTGGAAGAACCTGGGTTCATCTATACAAGGATTGGAAATC
CTACCGTCTCAGTTCTTGAAGAAAGAATAGCCGCCCTGGAAGAAGGGGTGGGAGCCTTAG
CGGTTGCCAGTGGACAAGCCGCTATAACTTACGCCATTTTGAACATCGCGGGCCCAGGAG
ATGAGATCGTCAGCGGGAGCGCGCTGTATGGGGGAACGTACAATCTGTTCAGACACACTC
TCTATAAAAAATCCGGCATCATCGTGAAGTTTGTGGATGAGACAGATCCAAAGAACATAG
AAGAGGCCATCACCGAGAAAACAAAGGCGGTGTACCTTGAAACTATCGGGAATCCCGGTC
TCACAGTGCCGGACTTTGAAGCGATAGCGGAGATCGCTCACAGACACGGTGTTCCTTTGA
TAGTGGACAATACGGTAGCTCCGTACATATTCAGGCCCTTCGAACACGGTGCCGACATCG
TTGTTTATTCGGCCACGAAATTCATCGGAGGACACGGAACATCGATAGGCGGTCTCATCG
TAGACAGCGGAAAATTCGACTGGACGAACGGAAAGTTTCCAGAACTCGTGGAACCAGATC
CCAGCTACCACGGTGTGAGTTATGTGGAGACGTTCAAAGAAGCAGCCTACATAGCAAAAT
GTAGAACCCAGCTTTTGAGGGACCTGGGAAGCTGTATGAGCCCGTTCAACGCGTTTCTGT
TCATCCTCGGACTTGAAACCCTCAGCTTGAGGATGAAGAAACACTGTGAAAACGCACTGA
AGATCGTTGAATTTCTGAAATCGCATCCCGCCGTGAGCTGGGTCAACTATCCGATAGCTG
AAGGCAATAAAACCAGAGAAAATGCGCTGAAATACCTCAAAGAAGGATACGGTGCGATTG
TAACGTTCGGTGTGAAAGGCGGAAAAGAGGCGGGAAAGAAGTTCATAGACAGTCTCACAC
TCATTTCCCACCTCGCCAACATTGGTGATGCAAGAACTCTGGCTATTCATCCCGCTTCGA
CAACCCATCAGCAGCTCACGGAAGAAGAGCAGTTGAAAACGGGTGTTACTCCGGATATGA
TAAGATTGTCTGTTGGAATAGAAGATGTGGAAGATATCATAGCCGATCTGGATCAGGCTC
TCAGAAAATCTCAGGAGGGATAATTAATTAACTTTAAGAAGGAGATATACATACCCGGGT
AAGTTTAAACGGTC
Protein sequence predicted based on nucleotide sequence
MDWKKYGYNTRALHAGYEPPEQATGSRAVPIYQTTSYVFRDSDHAARLFALEEPGFIYTR IGNPTVSVLEERIAALEEGVGALAVASGQAAITYAILNIAGPGDEIVSGSALYGGTYNLF RHTLYKKSGIIVKFVDETDPKNIEEAITEKTKAVYLETIGNPGLTVPDFEAIAEIAHRHG VPLIVDNTVAPYIFRPFEHGADIVVYSATKFIGGHGTSIGGLIVDSGKFDWTNGKFPELV EPDPSYHGVSYVETFKEAAYIAKCRTQLLRDLGSCMSPFNAFLFILGLETLSLRMKKHCE NALKIVEFLKSHPAVSWVNYPIAEGNKTRENALKYLKEGYGAIVTFGVKGGKEAGKKFID SLTLISHLANIGDARTLAIHPASTTHQQLTEEEQLKTGVTPDMIRLSVGIEDVEDIIADL DQALRKSQEG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 282751
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
