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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: 5430.1.40_GO.6760
Keyword: full-length cds (mutant)
Species: Arabidopsis thaliana Columbia
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Center for Eukaryotic Structural Genomics
AtCD00383677

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 540nts         Open reading frame : 51 to 590

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCCGACGTAGCAACAAAGCACCCCATGGAAGATGAGGTGAAGAAGACGG
AGGCGTCTAGTTTGGTGGGGAAGCTAGAGACAGACGTGGAGATCAAGGCTTCGGCTGATA
AGTTTCACCACATGTTCGCTGGGAAACCACACCATGTCTCCAAAGCAAGTCCAGGCAACA
TTCAGGGATGTGATCTGCACGAAGGCGACTGGGGCACAGTCGGCTCTATCGTCTTCTGGA
ATTACGTACATGATGGGGAGGCAAAGGTGGCGAAGGAGAGGATCGAGGCGGTGGAGCCAG
ATAAGAACTTGATCACGTTTAGGGTTATAGAAGGTGATCTGATGAAAGAGTACAAGAGCT
TCTTGCTCACCATCCAGGTGACCCCTAAGCCTGGAGGCCCTGGAAGTATTGTGCACTGGC
ACCTTGAGTATGAGAAAATTAGCGAGGAGGTAGCTCACCCGGAAACTCTCCTCCAGTTCT
GTGTCGAGGTCTCCAAAGAGATCGACGAACATCTTTTGGCCGAGGAATAGTAGGACCCAG
CTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
CCAACTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
GACCCAGCTTTCTTGTACAAAGTTGGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.6760
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Protein
  • Clone
  • Experimental

Protein

SMART domain : Bet_v_1 : Pathogenesis-related protein Bet v I family
Structural Annotation Wiki : TOPSAN

Clone

Cloning Information : 503158
HIP Master Clone ID : 414325
Original Clone ID : 5430.1.40_GO.6760

Experimental

TargetTrack - Experimental data : GO.6760