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UHMK1 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  UHMK1
Gene Name:  U2AF homology motif (UHM) kinase 1
Sequence             
Original Clone ID: FLH205315.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038439

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1035nts         Open reading frame : 1 to 1035
Reference Sequence Alignment

ATGGCGGGATCCGGCTGCGCCTGGGGCGCGGAGCCGCCGCGTTTTCTGGAGGCCTTCGGG
CGGCTGTGGCAGGTACAGAGCCGTCTGGGTAGCGGCTCCTCCGCCTCGGTGTATCGGGTT
CGCTGCTGCGGCAACCCTGGCTCGCCCCCCGGCGCCCTCAAGCAGTTCTTGCCGCCAGGA
ACCACCGGGGCTGCGGCCTCTGCCGCCGAGTATGGTTTCCGCAAAGAGAGGGCGGCGCTG
GAACAGTTGCAGGGTCACAGAAACATCGTGACTTTGTATGGAGTGTTTACAATCCACTTT
TCTCCAAATGTGCCATCACGCTGTCTGTTGCTTGAACTCCTGGATGTCAGTGTTTCGGAA
TTGCTCTTATATTCCAGTCACCAGGGTTGTTCCATGTGGATGATACAGCATTGTGCCCGA
GATGTTTTGGAGGCCCTTGCTTTTCTTCATCATGAGGGCTATGTCCATGCGGACCTCAAA
CCACGTAACATATTGTGGAGTGCAGAGAATGAATGTTTTAAACTCATTGACTTTGGACTT
AGCTTCAAAGAAGGCAATCAGGATGTAAAGTATATTCAGACAGACGGGTATCGGGCTCCA
GAAGCAGAATTGCAAAATTGCTTGGCCCAGGCTGGCCTGCAGAGTGATACAGAATGTACC
TCAGCTGTTGATCTGTGGAGCCTAGGAATCATTTTACTGGAAATGTTCTCAGGAATGAAA
CTGAAACATACAGTCAGATCTCAGGAATGGAAGGCAAACAGTTCTGCTATTATTGATCAC
ATATTTGCCAGTAAAGCAGTGGTGAATGCCGCAATTCCAGCCTATCACCTAAGAGACCTT
ATCAAAAGCATGCTTCATGATGATCCAAGCAGAAGAATTCCTGCTGAAATGGCATTGTGC
AGCCCATTCTTTAGCATTCCTTTTGCCCCTCATATTGAAGATCTGGTCATGCTTCCCACT
CCAGTGCTAAGACTGCTGAATGTGCTGGATGATGATTATCTTGAGAATGAAGAGGAATAT
GAAGGTCTTTGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1193
Start on reference sequence 159
Mutations:
None
Discrepancy:
c770t,T257I
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UHMK1
Symbol Nomenclature : UHMK1
SYNONYM : KIS; KIST; P-CIP2
Designation : KIS protein kinase|PAM COOH-terminal interactor protein 2|kinase interacting with leukemia-associated gene (stathmin)|serine/threonine-protein kinase Kist
Full Nomenclature : U2AF homology motif (UHM) kinase 1
GENEID : 127933
HGNC : 19683
MIM : 608849
Vega : OTTHUMG00000031373
Target GenBank: NM_144624

 

Reference Sequence Alignment

  

HsCD00038439        ATGGCGGGATCCGGCTGCGCCTGGGGCGCGGAGCCGCCGCGTTTTCTGGAGGCCTTCGGG
NM_175866.4         ATGGCGGGATCCGGCTGCGCCTGGGGCGCGGAGCCGCCGCGTTTTCTGGAGGCCTTCGGG
NM_001184763.1      ------------------------------------------------------------
NM_144624.2         ATGGCGGGATCCGGCTGCGCCTGGGGCGCGGAGCCGCCGCGTTTTCTGGAGGCCTTCGGG
                                                                                

HsCD00038439        CGGCTGTGGCAGGTACAGAGCCGTCTGGGTAGCGGCTCCTCCGCCTCGGTGTATCGGGTT
NM_175866.4         CGGCTGTGGCAGGTACAGAGCCGTCTGGGTAGCGGCTCCTCCGCCTCGGTGTATCGGGTT
NM_001184763.1      ------------------------------------------------------------
NM_144624.2         CGGCTGTGGCAGGTACAGAGCCGTCTGGGTAGCGGCTCCTCCGCCTCGGTGTATCGGGTT
                                                                                

HsCD00038439        CGCTGCTGCGGCAACCCTGGCTCGCCCCCCGGCGCCCTCAAGCAGTTCTTGCCGCCAGGA
NM_175866.4         CGCTGCTGCGGCAACCCTGGCTCGCCCCCCGGCGCCCTCAAGCAGTTCTTGCCGCCAGGA
NM_001184763.1      ------------------------------------------------------------
NM_144624.2         CGCTGCTGCGGCAACCCTGGCTCGCCCCCCGGCGCCCTCAAGCAGTTCTTGCCGCCAGGA
                                                                                

HsCD00038439        ACCACCGGGGCTGCGGCCTCTGCCGCCGAGTATGGTTTCCGCAAAGAGAGGGCGGCGCTG
NM_175866.4         ACCACCGGGGCTGCGGCCTCTGCCGCCGAGTATGGTTTCCGCAAAGAGAGGGCGGCGCTG
NM_001184763.1      ------------------------------------------ATGTTTCTTACCAGACCG
NM_144624.2         ACCACCGGGGCTGCGGCCTCTGCCGCCGAGTATGGTTTCCGCAAAGAGAGGGCGGCGCTG
                                                              *:. : .  .* . .* *

HsCD00038439        GAACAGTTGCAGGGTCACAGAAACATCGTGACTTTGTATGGAGTGTTTACAATCCACTTT
NM_175866.4         GAACAGTTGCAGGGTCACAGAAACATCGTGACTTTGTATGGAGTGTTTACAATCCACTTT
NM_001184763.1      AAGGTCTGTGTTGACCTTAACCGGAGAGTGACTTTGTATGGAGTGTTTACAATCCACTTT
NM_144624.2         GAACAGTTGCAGGGTCACAGAAACATCGTGACTTTGTATGGAGTGTTTACAATCCACTTT
                    .*. : *   : *. *: *.... * .*********************************

HsCD00038439        TCTCCAAATGTGCCATCACGCTGTCTGTTGCTTGAACTCCTGGATGTCAGTGTTTCGGAA
NM_175866.4         TCTCCAAATGTGCCATCACGCTGTCTGTTGCTTGAACTCCTGGATGTCAGTGTTTCGGAA
NM_001184763.1      TCTCCAAATGTGCCATCACGCTGTCTGTTGCTTGAACTCCTGGATGTCAGTGTTTCGGAA
NM_144624.2         TCTCCAAATGTGCCATCACGCTGTCTGTTGCTTGAACTCCTGGATGTCAGTGTTTCGGAA
                    ************************************************************

HsCD00038439        TTGCTCTTATATTCCAGTCACCAGGGTTGTTCCATGTGGATGATACAGCATTGTGCCCGA
NM_175866.4         TTGCTCTTATATTCCAGTCACCAGGGTTGTTCCATGTGGATGATACAGCATTGTGCCCGA
NM_001184763.1      TTGCTCTTATATTCCAGTCACCAGGGTTGTTCCATGTGGATGATACAGCATTGTGCCCGA
NM_144624.2         TTGCTCTTATATTCCAGTCACCAGGGTTGTTCCATGTGGATGATACAGCATTGTGCCCGA
                    ************************************************************

HsCD00038439        GATGTTTTGGAGGCCCTTGCTTTTCTTCATCATGAGGGCTATGTCCATGCGGACCTCAAA
NM_175866.4         GATGTTTTGGAGGCCCTTGCTTTTCTTCATCATGAGGGCTATGTCCATGCGGACCTCAAA
NM_001184763.1      GATGTTTTGGAGGCCCTTGCTTTTCTTCATCATGAGGGCTATGTCCATGCGGACCTCAAA
NM_144624.2         GATGTTTTGGAGGCCCTTGCTTTTCTTCATCATGAGGGCTATGTCCATGCGGACCTCAAA
                    ************************************************************

HsCD00038439        CCACGTAACATATTGTGGAGTGCAGAGAATGAATGTTTTAAACTCATTGACTTTGGACTT
NM_175866.4         CCACGTAACATATTGTGGAGTGCAGAGAATGAATGTTTTAAACTCATTGACTTTGGACTT
NM_001184763.1      CCACGTAACATATTGTGGAGTGCAGAGAATGAATGTTTTAAACTCATTGACTTTGGACTT
NM_144624.2         CCACGTAACATATTGTGGAGTGCAGAGAATGAATGTTTTAAACTCATTGACTTTGGACTT
                    ************************************************************

HsCD00038439        AGCTTCAAAGAAGGCAATCAGGATGTAAAGTATATTCAGACAGACGGGTATCGGGCTCCA
NM_175866.4         AGCTTCAAAGAAGGCAATCAGGATGTAAAGTATATTCAGACAGACGGGTATCGGGCTCCA
NM_001184763.1      AGCTTCAAAGAAGGCAATCAGGATGTAAAGTATATTCAGACAGACGGGTATCGGGCTCCA
NM_144624.2         AGCTTCAAAGAAGGCAATCAGGATGTAAAGTATATTCAGACAGACGGGTATCGGGCTCCA
                    ************************************************************

HsCD00038439        GAAGCAGAATTGCAAAATTGCTTGGCCCAGGCTGGCCTGCAGAGTGATACAGAATGTACC
NM_175866.4         GAAGCAGAATTGCAAAATTGCTTGGCCCAGGCTGGCCTGCAGAGTGATACAGAATGTACC
NM_001184763.1      GAAGCAGAATTGCAAAATTGCTTGGCCCAGGCTGGCCTGCAGAGTGATACAGAATGTACC
NM_144624.2         GAAGCAGAATTGCAAAATTGCTTGGCCCAGGCTGGCCTGCAGAGTGATACAGAATGTACC
                    ************************************************************

HsCD00038439        TCAGCTGTTGATCTGTGGAGCCTAGGAATCATTTTACTGGAAATGTTCTCAGGAATGAAA
NM_175866.4         TCAGCTGTTGATCTGTGGAGCCTAGGAATCATTTTACTGGAAATGTTCTCAGGAATGAAA
NM_001184763.1      TCAGCTGTTGATCTGTGGAGCCTAGGAATCATTTTACTGGAAATGTTCTCAGGAATGAAA
NM_144624.2         TCAGCTGTTGATCTGTGGAGCCTAGGAATCATTTTACTGGAAATGTTCTCAGGAATGAAA
                    ************************************************************

HsCD00038439        CTGAAACATACAGTCAGATCTCAGGAATGGAAGGCAAACAGTTCTGCTATTATTGATCAC
NM_175866.4         CTGAAACATACAGTCAGATCTCAGGAATGGAAGGCAAACAGTTCTGCTATTATTGATCAC
NM_001184763.1      CTGAAACATACAGTCAGATCTCAGGAATGGAAGGCAAACAGTTCTGCTATTATTGATCAC
NM_144624.2         CTGAAACATACAGTCAGATCTCAGGAATGGAAGGCAAACAGTTCTGCTATTATTGATCAC
                    ************************************************************

HsCD00038439        ATATTTGCCAGTAAAGCAGTGGTGAATGCCGCAATTCCAGCCTATCACCTAAGAGACCTT
NM_175866.4         ATATTTGCCAGTAAAGCAGTGGTGAATGCCGCAATTCCAGCCTATCACCTAAGAGACCTT
NM_001184763.1      ATATTTGCCAGTAAAGCAGTGGTGAATGCCGCAATTCCAGCCTATCACCTAAGAGACCTT
NM_144624.2         ATATTTGCCAGTAAAGCAGTGGTGAATGCCGCAATTCCAGCCTATCACCTAAGAGACCTT
                    ************************************************************

HsCD00038439        ATCAAAAGCATGCTTCATGATGATCCAAGCAGAAGAATTCCTGCTGAAATGGCATTGTGC
NM_175866.4         ATCAAAAGCATGCTTCATGATGATCCAAGCAGAAGAATTCCTGCTGAAATGGCATTGTGC
NM_001184763.1      ATCAAAAGCATGCTTCATGATGATCCAAGCAGAAGAATTCCTGCTGAAATGGCATTGTGC
NM_144624.2         ATCAAAAGCATGCTTCATGATGATCCAAGCAGAAGAATTCCTGCTGAAATGGCATTGTGC
                    ************************************************************

HsCD00038439        AGCCCATTCTTTAGCATTCCTTTTGCCCCTCATATTGAAGATCTGGTCATGCTTCCCACT
NM_175866.4         AGCCCATTCTTTAGCATTCCTTTTGCCCCTCATATTGAAGATCTGGTCATGCTTCCCACT
NM_001184763.1      AGCCCATTCTTTAGCATTCCTTTTGCCCCTCATATTGAAGATCTGGTCATGCTTCCCACT
NM_144624.2         AGCCCATTCTTTAGCATTCCTTTTGCCCCTCATATTGAAGATCTGGTCATGCTTCCCACT
                    ************************************************************

HsCD00038439        CCAGTGCTAAGACTGCTGAATGTGCTGGATGATGATTATCTTGAGAATGAAGAGGAATAT
NM_175866.4         CCAGTGCTAAGACTGCTGAATGTGCTGGATGATGATTATCTTGAGAATGAAGAGGAATAT
NM_001184763.1      CCAGTGCTAAGACTGCTGAATGTGCTGGATGATGATTATCTTGAGAATGAAGAGGAATAT
NM_144624.2         CCAGTGCTAAGACTGCTGAATGTGCTGGATGATGATTATCTTGAGAATGAAGAGGAATAT
                    ************************************************************

HsCD00038439        GAAGGTCTTTGTTTG---------------------------------------------
NM_175866.4         GAAGATGTTGTAGAAGATGTAAAAGAGGAGTGTCAAAAATATGGACCAGTGGTATCTCTA
NM_001184763.1      GAAGATGTTGTAGAAGATGTAAAAGAGGAGTGTCAAAAATATGGACCAGTGGTATCTCTA
NM_144624.2         GAAGGTCTTTGTTGA---------------------------------------------
                    ****.* **  :  .                                             

HsCD00038439        ------------------------------------------------------------
NM_175866.4         CTTGTTCCAAAGGAAAATCCTGGCAGAGGACAAGTCTTTGTTGAGTATGCAAATGCTGGT
NM_001184763.1      CTTGTTCCAAAGGAAAATCCTGGCAGAGGACAAGTCTTTGTTGAGTATGCAAATGCTGGT
NM_144624.2         ------------------------------------------------------------
                                                                                

HsCD00038439        ------------------------------------------------------------
NM_175866.4         GATTCCAAAGCTGCGCAGAAATTACTGACTGGAAGGATGTTTGATGGGAAGTTTGTTGTG
NM_001184763.1      GATTCCAAAGCTGCGCAGAAATTACTGACTGGAAGGATGTTTGATGGGAAGTTTGTTGTG
NM_144624.2         ------------------------------------------------------------
                                                                                

HsCD00038439        ------------------------------------------------------------
NM_175866.4         GCTACATTCTACCCGCTGAGTGCCTACAAGAGGGGATATCTGTATCAAACCTTGCTTTAA
NM_001184763.1      GCTACATTCTACCCGCTGAGTGCCTACAAGAGGGGATATCTGTATCAAACCTTGCTTTAA
NM_144624.2         ------------------------------------------------------------
                                                                                

Clone

Cloning Information : 205315
HIP Master Clone ID : 177812
Original Clone ID : FLH205315.01L

Organism


TAX_ID : 9606
Species Specific ID: 127933

Genome

Chromosome : 1
Map Location : 1q23.3
Ensembl : ENSG00000152332

Reagents

Labome : UHMK1-antibody