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CDKL1 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CDKL1
Gene Name:  cyclin-dependent kinase-like 1 (CDC2-related kinase)
Sequence             
Original Clone ID: FLH205316.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038440

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 906nts         Open reading frame : 1 to 906
Reference Sequence Alignment

ATGATGGAGAAGTATGAAAAAATTGGGAAAATTGGAGAAGGATCCTATGGAGTTGTTTTC
AAATGTAGAAACAGGGACACGGGTCAGATTGTGGCCATCAAGAAGTTTCTGGAATCAGAA
GATGACCCTGTCATAAAGAAAATTGCCCTTCGGGAAATCCGAATGCTCAAGCAACTCAAG
CATCCCAACCTTGTTAACCTCCTGGAAGTCTTCAGGAGGAAACGGAGGCTTCACCTGGTG
TTTGAATATTGTGACCACACAGTTCTCCATGAGTTGGACAGATACCAAAGAGGGGTACCA
GAACATCTCGTGAAGAGCATAACTTGGCAGACACTGCAAGCTGTAAATTTTTGCCATAAA
CACAATTGCATACATAGAGACGTGAAGCCAGAAAATATCCTCATCACGAAACATTCCGTG
ATTAAGCTTTGTGACTTTGGATTTGCTCGGCTTTTGACTGGACCGAGTGACTACTATACA
GACTACGTGGCTACCAGGTGGTACCGCTCCCCTGAGCTGCTGGTGGGGGACACGCAGTAC
GGCCCCCCGGTGGATGTTTGGGCAATTGGCTGTGTCTTTGCTGAGCTGCTGTCAGGAGTG
CCTCTGTGGCCAGGAAAATCGGATGTGGATCAGCTGTATCTGATTAGGAAGACCTTGGGG
GATCTCATTCCTAGGCACCAGCAAGTGTTTAGCACGAATCAGTACTTCAGTGGAGTGAAA
ATTCCAGACCCTGAAGATATGGAACCACTTGAATTAAAATTCCCAAACATCTCTTATCCT
GCCCTGGGGCTCCTAAAGTTGCAGTACCTACCCCAGCTAACTGGCAGCAGCATCCTTCCA
GCTTTGGATAATAAGAAGTACTACTGTGATACCAAGAAACTTAACTACCGTTTTCCAAAC
ATTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1105
Start on reference sequence 29
Mutations:
None
Discrepancy:
del@796,171;
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDKL1
Symbol Nomenclature : CDKL1
SYNONYM : KKIALRE; P42
Designation : CDC2-related kinase 1|cyclin-dependent kinase-like 1|protein kinase p42 KKIALRE|serine/threonine protein kinase KKIALRE|serine/threonine-protein kinase KKIALRE
Full Nomenclature : cyclin-dependent kinase-like 1 (CDC2-related kinase)
GENEID : 8814
HGNC : 1781
MIM : 603441
Target GenBank: NM_004196

 

Reference Sequence Alignment

  

HsCD00038440        ATGATGGAGAAGTATGAAAAAATTGGGAAAATTGGAGAAGGATCCTATGGAGTTGTTTTC
NM_004196.4         ATGATGGAGAAGTATGAAAAAATTGGGAAAATTGGAGAAGGATCCTATGGAGTTGTTTTC
XM_005268157.1      ATGATGGAGAAGTATGAAAAAATTGGGAAAATTGGAGAAGGATCCTATGGAGTTGTTTTC
XM_005268159.1      ATGATGGAGAAGTATGAAAAAATTGGGAAAATTGGAGAAGGATCCTATGGAGTTGTTTTC
                    ************************************************************

HsCD00038440        AAATGTAGAAACAGGGACACGGGTCAGATTGTGGCCATCAAGAAGTTTCTGGAATCAGAA
NM_004196.4         AAATGTAGAAACAGGGACACGGGTCAGATTGTGGCCATCAAGAAGTTTCTGGAATCAGAA
XM_005268157.1      AAATGTAGAAACAGGGACACGGGTCAGATTGTGGCCATCAAGAAGTTTCTGGAATCAGAA
XM_005268159.1      AAATGTAGAAACAGGGACACGGGTCAGATTGTGGCCATCAAGAAGTTTCTGGAATCAGAA
                    ************************************************************

HsCD00038440        GATGACCCTGTCATAAAGAAAATTGCCCTTCGGGAAATCCGAATGCTCAAGCAACTCAAG
NM_004196.4         GATGACCCTGTCATAAAGAAAATTGCCCTTCGGGAAATCCGAATGCTCAAGCAACTCAAG
XM_005268157.1      GATGACCCTGTCATAAAGAAAATTGCCCTTCGGGAAATCCGAATGCTCAAGCAACTCAAG
XM_005268159.1      GATGACCCTGTCATAAAGAAAATTGCCCTTCGGGAAATCCGAATGCTCAAGCAACTCAAG
                    ************************************************************

HsCD00038440        CATCCCAACCTTGTTAACCTCCTGGAAGTCTTCAGGAGGAAACGGAGGCTTCACCTGGTG
NM_004196.4         CATCCCAACCTTGTTAACCTCCTGGAAGTCTTCAGGAGGAAACGGAGGCTTCACCTGGTG
XM_005268157.1      CATCCCAACCTTGTTAACCTCCTGGAAGTCTTCAGGAGGAAACGGAGGCTTCACCTGGTG
XM_005268159.1      CATCCCAACCTTGTTAACCTCCTGGAAGTCTTCAGGAGGAAACGGAGGCTTCACCTGGTG
                    ************************************************************

HsCD00038440        TTTGAATATTGTGACCACACAGTTCTCCATGAGTTGGACAGATACCAAAGAGGGGTACCA
NM_004196.4         TTTGAATATTGTGACCACACAGTTCTCCATGAGTTGGACAGATACCAAAGAGGGGTACCA
XM_005268157.1      TTTGAATATTGTGACCACACAGTTCTCCATGAGTTGGACAGATACCAAAGAGGGGTACCA
XM_005268159.1      TTTGAATATTGTGACCACACAGTTCTCCATGAGTTGGACAGATACCAAAGAGGGGTACCA
                    ************************************************************

HsCD00038440        GAACATCTCGTGAAGAGCATAACTTGGCAGACACTGCAAGCTGTAAATTTTTGCCATAAA
NM_004196.4         GAACATCTCGTGAAGAGCATAACTTGGCAGACACTGCAAGCTGTAAATTTTTGCCATAAA
XM_005268157.1      GAACATCTCGTGAAGAGCATAACTTGGCAGACACTGCAAGCTGTAAATTTTTGCCATAAA
XM_005268159.1      GAACATCTCGTGAAGAGCATAACTTGGCAGACACTGCAAGCTGTAAATTTTTGCCATAAA
                    ************************************************************

HsCD00038440        CACAATTGCATACATAGAGACGTGAAGCCAGAAAATATCCTCATCACGAAACATTCCGTG
NM_004196.4         CACAATTGCATACATAGAGACGTGAAGCCAGAAAATATCCTCATCACGAAACATTCCGTG
XM_005268157.1      CACAATTGCATACATAGAGACGTGAAGCCAGAAAATATCCTCATCACGAAACATTCCGTG
XM_005268159.1      CACAATTGCATACATAGAGACGTGAAGCCAGAAAATATCCTCATCACGAAACATTCCGTG
                    ************************************************************

HsCD00038440        ATTAAGCTTTGTGACTTTGGATTTGCTCGGCTTTTGACTGGACCGAGTGACTACTATACA
NM_004196.4         ATTAAGCTTTGTGACTTTGGATTTGCTCGGCTTTTGACTGGACCGAGTGACTACTATACA
XM_005268157.1      ATTAAGCTTTGTGACTTTGGATTTGCTCGGCTTTTGACTGGACCGAGTGACTACTATACA
XM_005268159.1      ATTAAGCTTTGTGACTTTGGATTTGCTCGGCTTTTGACTGGACCGAGTGACTACTATACA
                    ************************************************************

HsCD00038440        GACTACGTGGCTACCAGGTGGTACCGCTCCCCTGAGCTGCTGGTGGGGGACACGCAGTAC
NM_004196.4         GACTACGTGGCTACCAGGTGGTACCGCTCCCCTGAGCTGCTGGTGGGGGACACGCAGTAC
XM_005268157.1      GACTACGTGGCTACCAGGTGGTACCGCTCCCCTGAGCTGCTGGTGGGGGACACGCAGTAC
XM_005268159.1      GACTACGTGGCTACCAGGTGGTACCGCTCCCCTGAGCTGCTGGTGGGGGACACGCAGTAC
                    ************************************************************

HsCD00038440        GGCCCCCCGGTGGATGTTTGGGCAATTGGCTGTGTCTTTGCTGAGCTGCTGTCAGGAGTG
NM_004196.4         GGCCCCCCGGTGGATGTTTGGGCAATTGGCTGTGTCTTTGCTGAGCTGCTGTCAGGAGTG
XM_005268157.1      GGCCCCCCGGTGGATGTTTGGGCAATTGGCTGTGTCTTTGCTGAGCTGCTGTCAGGAGTG
XM_005268159.1      GGCCCCCCGGTGGATGTTTGGGCAATTGGCTGTGTCTTTGCTGAGCTGCTGTCAGGAGTG
                    ************************************************************

HsCD00038440        CCTCTGTGGCCAGGAAAATCGGATGTGGATCAGCTGTATCTGATTAGGAAGACCTTGGGG
NM_004196.4         CCTCTGTGGCCAGGAAAATCGGATGTGGATCAGCTGTATCTGATTAGGAAGACCTTGGGG
XM_005268157.1      CCTCTGTGGCCAGGAAAATCGGATGTGGATCAGCTGTATCTGATTAGGAAGACCTTGGGG
XM_005268159.1      CCTCTGTGGCCAGGAAAATCGGATGTGGATCAGCTGTATCTGATTAGGAAGACCTTGGGG
                    ************************************************************

HsCD00038440        GATCTCATTCCTAGGCACCAGCAAGTGTTTAGCACGAATCAGTACTTCAGTGGAGTGAAA
NM_004196.4         GATCTCATTCCTAGGCACCAGCAAGTGTTTAGCACGAATCAGTACTTCAGTGGAGTGAAA
XM_005268157.1      GATCTCATTCCTAGGCACCAGCAAGTGTTTAGCACGAATCAGTACTTCAGTGGAGTGAAA
XM_005268159.1      GATCTCATTCCTAGGCACCAGCAAGTGTTTAGCACGAATCAGTACTTCAGTGGAGTGAAA
                    ************************************************************

HsCD00038440        ATTCCAGACCCTGAAGATATGGAACCACTTGAATTAAAATTCCCAAACATCTCTTATCCT
NM_004196.4         ATTCCAGACCCTGAAGATATGGAACCACTTGAATTAAAATTCCCAAACATCTCTTATCCT
XM_005268157.1      ATTCCAGACCCTGAAGATATGGAACCACTTGAATTAAAATTCCCAAACATCTCTTATCCT
XM_005268159.1      ATTCCAGACCCTGAAGATATGGAACCACTTGAATTAAAATTCCCAAACATCTCTTATCCT
                    ************************************************************

HsCD00038440        GCCCTGGGGCTCCTAAAG------------------------------------------
NM_004196.4         GCCCTGGGGCTCCTAAAGGGCTGTCTCCACATGGACCCTACTCAAAGGCTGACATGTGAA
XM_005268157.1      GCCCTGGGGCTCCTAAAGGGCTGTCTCCACATGGACCCTACTCAAAGGCTGACATGTGAA
XM_005268159.1      GCCCTGGGGCTCCTAAAG------------------------------------------
                    ******************                                          

HsCD00038440        ------------------------------------------------------------
NM_004196.4         CAGCTGTTGCATCACCCATATTTTGAAAACATCAGAGAAATAGAGGATTTGGCAAAAGAA
XM_005268157.1      CAGCTGTTGCATCACCCATATTTTGAAAACATCAGAGAAATAGAGGATTTGGCAAAAGAA
XM_005268159.1      ------------------------------------------------------------
                                                                                

HsCD00038440        ------------------------------------------------------------
NM_004196.4         CACAACAAACCAACAAGGAAGACCCTAAGAAAGAGCCGAAAGCACCACTGCTTTACAGAA
XM_005268157.1      CACAACAAACCAACAAGGAAGACCCTAAGAAAGAGCCGAAAGCACCACTGCTTTACAGAA
XM_005268159.1      ------------------------------------------------------------
                                                                                

HsCD00038440        ---------TTGCAGTACCTACCCCAGCTAACTGGCAGCAGCATCCTTCCAGCTTTGGAT
NM_004196.4         ACATCCAAGTTGCAGTACCTACCCCAGCTAACTGGCAGCAGCATCCTTCCAGCTTTGGAT
XM_005268157.1      ACATCCAAGTTGCAGTACCTACCCCAGCTAACTGGCAGCAGCATCCTTCCAGCTTTGGAT
XM_005268159.1      ---------TTGCAGTACCTACCCCAGCTAACTGGCAGCAGCATCCTTCCAGCTTTGGAT
                             ***************************************************

HsCD00038440        AATAAGAAGTACTACTGTGATACCAAGAAACTTAACTACCGTTTTCCAAACATTTTG
NM_004196.4         AATAAGAAGTACTACTGTGATACCAAGAAACTTAACTACCGTTTTCCAAACATTTAA
XM_005268157.1      AATAAGAAGTACTACTGTGATACCAAGAAACTTAACTACCGTTTTCCAAACATTTAA
XM_005268159.1      AATAAGAAGTACTACTGTGATACCAAGAAACTTAACTACCGTTTTCCAAACATTTAA
                    *******************************************************:.

Clone

Cloning Information : 205316
HIP Master Clone ID : 177821
Original Clone ID : FLH205316.01L

Organism


TAX_ID : 9606
Species Specific ID: 8814

Genome

Chromosome : 14
Map Location : 14q21.3

Reagents

Labome : CDKL1-antibody