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ALS2CR7 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CDK15
Gene Name:  cyclin-dependent kinase 15
Sequence             
Original Clone ID: FLH205318.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00038442

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1155nts         Open reading frame : 1 to 1155
Reference Sequence Alignment

ATGACTTCATTTCACCCCAGGGGACTTCAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAA
AGGCCACGGAGTAACAGTGATTGTTTTCAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGG
AGGAAGAGCCTCCCTTTTGGGGCAGCCTCATCTTACTTGAACTTGGAGAAGCTGGGTGAA
GGCTCTTATGCGACAGTTTACAAGGGGATTAGCAGAATAAATGGACAACTAGTGGCTTTA
AAAGTCATCAGCATGAATGCAGAGGAAGGAGTCCCATTTACAGCTATCCGAGAAGCTTCT
CTCCTGAAGGGTTTGAAACATGCCAATATTGTGCTCCTGCATGACATAATCCACACCAAA
GAGACACTGACATTCGTTTTTGAATACATGCACACAGACCTGGCCCAGTATATGTCTCAG
CATCCAGGAGGGCTTCATCCTCATAATGTCAGACTTTTCATGTTTCAACTTTTGCGGGGC
CTGGCGTACATCCACCACCAACACGTTCTTCACAGGGACCTGAAACCTCAGAACTTACTC
ATCAGTCACCTGGGAGAGCTCAAACTGGCTGATTTTGGTCTTGCCCGGGCCAAGTCCATT
CCCAGCCAGACATACTCTTCAGAAGTCGTGACCCTCTGGTACCGGCCCCCTGATGCTTTG
CTGGGAGCCACTGAATATTCCTCTGAGCTGGACATATGGGGTGCAGGCTGCATCTTTATT
GAAATGTTCCAGGGTCAACCTTTGTTTCCTGGGGTTTCCAACATCCTTGAACAGCTGGAG
AAAATCTGGGAGGTGCTGGGAGTCCCTACAGAGGATACTTGGCCGGGAGTCTCCAAGCTA
CCTAACTACAATCCAGAATGGTTCCCACTGCCTACGCCTCGAAGCCTTCATGTTGTCTGG
AACAGGCTGGGCAGGGTTCCTGAAGCTGAAGACCTGGCCTCCCAGATGCTAAAAGGCTTT
CCCAGAGACCGCGTCTCCGCCCAGGAAGCACTTGTTCATGATTATTTCAGCGCCCTGCCA
TCTCAGCTGTACCAGCTTCCTGATGAGGAGTCTTTGTTTACAGTTTCAGGAGTGAGGCTA
AAGCCAGAAATGTGTGACCTTTTGGCCTCCTACCAGAAAGGTCACCACCCAGCCCAGTTT
AGCAAATGCTGGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1258
Start on reference sequence 104
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDK15
Symbol Nomenclature : CDK15
SYNONYM : ALS2CR7; PFTAIRE2; PFTK2
Designation : PFTAIRE protein kinase 2|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7|amyotrophic lateral sclerosis 2 chromosomal region candidate gene 7 protein|cell division protein kinase 15|serine/threonine protein kinase|serine/threonine-protein kinase ALS2CR7|serine/threonine-protein kinase PFTAIRE-2
Full Nomenclature : cyclin-dependent kinase 15
GENEID : 65061
HGNC : 14434
Vega : OTTHUMG00000132838
Target GenBank: NM_139158

 

Reference Sequence Alignment

  

HsCD00038442        ------------------------------------------------------------
NM_139158.2         ------------------------------------------------------------
NM_001261436.1      ATGGGTCAAGAGCTGTGTGCAAAGACTGTACAGCCTGGATGCAGCTGCTACCATTGTTCA
NM_001261435.1      ATGGGTCAAGAGCTGTGTGCAAAGACTGTACAGCCTGGATGCAGCTGCTACCATTGTTCA
                                                                                

HsCD00038442        ------------------------------------------------------------
NM_139158.2         ------------------------------------------------------------
NM_001261436.1      GAGGGAGGCGAGGCACACAGCTGTCGGAGGAGTCAGCCTGAGACCACGGAGGCTGCGTTC
NM_001261435.1      GAGGGAGGCGAGGCACACAGCTGTCGGAGGAGTCAGCCTGAGACCACGGAGGCTGCGTTC
                                                                                

HsCD00038442        ---------------------------------ATGACTTCATTTCACCCCAGGGGACTT
NM_139158.2         ---------------------------------ATGACTTCATTTCACCCCAGGGGACTT
NM_001261436.1      AAGCTAACAGACCTAAAAGAAGCATCATGTTCCATGACTTCATTTCACCCCAGGGGACTT
NM_001261435.1      AAGCTAACAGACCTAAAAGAAGCATCATGTTCCATGACTTCATTTCACCCCAGGGGACTT
                                                     ***************************

HsCD00038442        CAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAAAGGCCACGGAGTAACAGTGATTGTTTT
NM_139158.2         CAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAAAGGCCACGGAGTAACAGTGATTGTTTT
NM_001261436.1      CAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAAAGGCCACGGAGTAACAGTGATTGTTTT
NM_001261435.1      CAAGCTGCCCGTGCCCAGAAGTTCAAGAGTAAAAGGCCACGGAGTAACAGTGATTGTTTT
                    ************************************************************

HsCD00038442        CAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGGAGGAAGAGCCTCCCTTTTGGGGCAGCC
NM_139158.2         CAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGGAGGAAGAGCCTCCCTTTTGGGGCAGCC
NM_001261436.1      CAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGGAGGAAGAGCCTCCCTTTTGGGGCAGCC
NM_001261435.1      CAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGGAGGAAGAGCCTCCCTTTTGGGGCAGCC
                    ************************************************************

HsCD00038442        TCATCTTACTTGAACTTGGAGAAGCTGGGTGAAGGCTCTTATGCGACAGTTTACAAGGGG
NM_139158.2         TCATCTTACTTGAACTTGGAGAAGCTGGGTGAAGGCTCTTATGCGACAGTTTACAAGGGG
NM_001261436.1      TCATCTTACTTGAACTTGGAGAAGCTGGGTGAAGGCTCTTATGCGACAGTTTACAAGGGG
NM_001261435.1      TCATCTTACTTGAACTTGGAGAAGCTGGGTGAAGGCTCTTATGCGACAGTTTACAAGGGG
                    ************************************************************

HsCD00038442        ATTAGCAGAATAAATGGACAACTAGTGGCTTTAAAAGTCATCAGCATGAATGCAGAGGAA
NM_139158.2         ATTAGCAGAATAAATGGACAACTAGTGGCTTTAAAAGTCATCAGCATGAATGCAGAGGAA
NM_001261436.1      ATTAGCAGAATAAATGGACAACTAGTGGCTTTAAAAGTCATCAGCATGAATGCAGAGGAA
NM_001261435.1      ATTAGCAGAATAAATGGACAACTAGTGGCTTTAAAAGTCATCAGCATGAATGCAGAGGAA
                    ************************************************************

HsCD00038442        GGAGTCCCATTTACAGCTATCCGAGAAGCTTCTCTCCTGAAGGGTTTGAAACATGCCAAT
NM_139158.2         GGAGTCCCATTTACAGCTATCCGAGAAGCTTCTCTCCTGAAGGGTTTGAAACATGCCAAT
NM_001261436.1      GGAGTCCCATTTACAGCTATCCGAGAAGCTTCTCTCCTGAAGGGTTTGAAACATGCCAAT
NM_001261435.1      GGAGTCCCATTTACAGCTATCCGAGAAGCTTCTCTCCTGAAGGGTTTGAAACATGCCAAT
                    ************************************************************

HsCD00038442        ATTGTGCTCCTGCATGACATAATCCACACCAAAGAGACACTGACATTCGTTTTTGAATAC
NM_139158.2         ATTGTGCTCCTGCATGACATAATCCACACCAAAGAGACACTGACATTCGTTTTTGAATAC
NM_001261436.1      ATTGTGCTCCTGCATGACATAATCCACACCAAAGAGACACTGACATTCGTTTTTGAATAC
NM_001261435.1      ATTGTGCTCCTGCATGACATAATCCACACCAAAGAGACACTGACATTCGTTTTTGAATAC
                    ************************************************************

HsCD00038442        ATGCACACAGACCTGGCCCAGTATATGTCTCAGCATCCAGGAGGGCTTCATCCTCATAAT
NM_139158.2         ATGCACACAGACCTGGCCCAGTATATGTCTCAGCATCCAGGAGGGCTTCATCCTCATAAT
NM_001261436.1      ATGCACACAGACCTGGCCCAGTATATGTCTCAGCATCCAGGAGGGCTTCATCCTCATAAT
NM_001261435.1      ATGCACACAGACCTGGCCCAGTATATGTCTCAGCATCCAGGAGGGCTTCATCCTCATAAT
                    ************************************************************

HsCD00038442        GTCAGACTTTTCATGTTTCAACTTTTGCGGGGCCTGGCGTACATCCACCACCAACACGTT
NM_139158.2         GTCAGACTTTTCATGTTTCAACTTTTGCGGGGCCTGGCGTACATCCACCACCAACACGTT
NM_001261436.1      GTCAGACTTTTCATGTTTCAACTTTTGCGGGGCCTGGCGTACATCCACCACCAACACGTT
NM_001261435.1      GTCAGACTTTTCATGTTTCAACTTTTGCGGGGCCTGGCGTACATCCACCACCAACACGTT
                    ************************************************************

HsCD00038442        CTTCACAGGGACCTGAAACCTCAGAACTTACTCATCAGTCACCTGGGAGAGCTCAAACTG
NM_139158.2         CTTCACAGGGACCTGAAACCTCAGAACTTACTCATCAGTCACCTGGGAGAGCTCAAACTG
NM_001261436.1      CTTCACAGGGACCTGAAACCTCAGAACTTACTCATCAGTCACCTGGGAGAGCTCAAACTG
NM_001261435.1      CTTCACAGGGACCTGAAACCTCAGAACTTACTCATCAGTCACCTGGGAGAGCTCAAACTG
                    ************************************************************

HsCD00038442        GCTGATTTTGGTCTTGCCCGGGCCAAGTCCATTCCCAGCCAGACATACTCTTCAGAAGTC
NM_139158.2         GCTGATTTTGGTCTTGCCCGGGCCAAGTCCATTCCCAGCCAGACATACTCTTCAGAAGTC
NM_001261436.1      GCTGATTTTGGTCTTGCCCGGGCCAAGTCCATTCCCAGCCAGACATACTCTTCAGAAGTC
NM_001261435.1      GCTGATTTTGGTCTTGCCCGGGCCAAGTCCATTCCCAGCCAGACATACTCTTCAGAAGTC
                    ************************************************************

HsCD00038442        GTGACCCTCTGGTACCGGCCCCCTGATGCTTTGCTGGGAGCCACTGAATATTCCTCTGAG
NM_139158.2         GTGACCCTCTGGTACCGGCCCCCTGATGCTTTGCTGGGAGCCACTGAATATTCCTCTGAG
NM_001261436.1      GTGACCCTCTGGTACCGGCCCCCTGATGCTTTGCTGGGAGCCACTGAATATTCCTCTGAG
NM_001261435.1      GTGACCCTCTGGTACCGGCCCCCTGATGCTTTGCTGGGAGCCACTGAATATTCCTCTGAG
                    ************************************************************

HsCD00038442        CTGGACATATGGGGTGCAGGCTGCATCTTTATTGAAATGTTCCAGGGTCAACCTTTGTTT
NM_139158.2         CTGGACATATGGGGTGCAGGCTGCATCTTTATTGAAATGTTCCAGGGTCAACCTTTGTTT
NM_001261436.1      CTGGACATATGGGGTGCAGGCTGCATCTTTATTGAAATGTTCCAGGGTCAACCTTTGTTT
NM_001261435.1      CTGGACATATGGGGTGCAGGCTGCATCTTTATTGAAATGTTCCAGGGTCAACCTTTGTTT
                    ************************************************************

HsCD00038442        CCTGGGGTTTCCAACATCCTTGAACAGCTGGAGAAAATCTGGGAGGTGCTGGGAGTCCCT
NM_139158.2         CCTGGGGTTTCCAACATCCTTGAACAGCTGGAGAAAATCTGGGAGGTGCTGGGAGTCCCT
NM_001261436.1      CCTGGGGTTTCCAACATCCTTGAACAGCTGGAGAAAATCTGGGAGGTGCTGGGAGTCCCT
NM_001261435.1      CCTGGGGTTTCCAACATCCTTGAACAGCTGGAGAAAATCTGGGAGGTGCTGGGAGTCCCT
                    ************************************************************

HsCD00038442        ACAGAGGATACTTGGCCGGGAGTCTCCAAGCTACCTAACTACAATCCAGAATGGTTCCCA
NM_139158.2         ACAGAGGATACTTGGCCGGGAGTCTCCAAGCTACCTAACTACAATCCAGAATGGTTCCCA
NM_001261436.1      ACAGAGGATACTTGGCCGGGAGTCTCCAAGCTACCTAACTACAATCCAGAATGGTTCCCA
NM_001261435.1      ACAGAGGATACTTGGCCGGGAGTCTCCAAGCTACCTAACTACAATCCAGAATGGTTCCCA
                    ************************************************************

HsCD00038442        CTGCCTACGCCTCGAAGCCTTCATGTTGTCTGGAACAGGCTGGGCAGGGTTCCTGAAGCT
NM_139158.2         CTGCCTACGCCTCGAAGCCTTCATGTTGTCTGGAACAGGCTGGGCAGGGTTCCTGAAGCT
NM_001261436.1      CTGCCTACGCCTCGAAGCCTTCATGTTGTCTGGAACAGGCTGGGCAGGGTTCCTGAAGCT
NM_001261435.1      CTGCCTACGCCTCGAAGCCTTCATGTTGTCTGGAACAGGCTGGGCAGGGTTCCTGAAGCT
                    ************************************************************

HsCD00038442        GAAGACCTGGCCTCCCAGATGCTAAAAGGCTTTCCCAGAGACCGCGTCTCCGCCCAGGAA
NM_139158.2         GAAGACCTGGCCTCCCAGATGCTAAAAGGCTTTCCCAGAGACCGCGTCTCCGCCCAGGAA
NM_001261436.1      GAAGACCTGGCCTCCCAGATGCTAAAAGGCTTTCCCAGAGACCGCGTCTCCGCCCAGGAA
NM_001261435.1      GAAGACCTGGCCTCCCAGATGCTAAAAGGCTTTCCCAGAGACCGCGTCTCCGCCCAGGAA
                    ************************************************************

HsCD00038442        GCACTTGTTCATGATTATTTCAGCGCCCTGCCATCTCAGCTGTACCAGCTTCCTGATGAG
NM_139158.2         GCACTTGTTCATGATTATTTCAGCGCCCTGCCATCTCAGCTGTACCAGCTTCCTGATGAG
NM_001261436.1      GCACTTGTTCATGATTATTTCAGCGCCCTGCCATCTCAGCTGTACCAGCTTCCTGATGAG
NM_001261435.1      GCACTTGTTCATGATTATTTCAGCGCCCTGCCATCTCAGCTGTACCAGCTTCCTGATGTT
                    **********************************************************: 

HsCD00038442        GAGTCTTTGTTTACAGTTTCAGGAGTGAGGCTAAAGCCAGAAATGTGTGACCTTTTGGCC
NM_139158.2         GAGTCTTTGTTTACAGTTTCAGGAGTGAGGCTAAAGCCAGAAATGTGTGACCTTTTGGCC
NM_001261436.1      TGA---------------------------------------------------------
NM_001261435.1      TCAGGAGTGAGGCTAAAGCCAGAAATGTGTGACCTTTTGGCCTCCTACCAGAAAGGTCAC
                      .                                                         

HsCD00038442        TCCTACCAGAAAGGTCACCACCCAGCCCAGTTTAGCAAATGCTGGTTG
NM_139158.2         TCCTACCAGAAAGGTCACCACCCAGCCCAGTTTAGCAAATGCTGGTGA
NM_001261436.1      ------------------------------------------------
NM_001261435.1      CACCCAGCCCAGTTTAGCAAATGCTGGTGA------------------
                                                                    

Clone

Cloning Information : 205318
HIP Master Clone ID : 177830
Original Clone ID : FLH205318.01L

Organism


TAX_ID : 9606
Species Specific ID: 65061

Genome

Chromosome : 2
Map Location : 2q33.2
Ensembl : ENSG00000138395

Reagents

Labome : CDK15-antibody