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Detailed Plasmid Information

TOP

Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: ER559-21.1
Keyword: None
Species: Escherichia coli
Type: cDNA
Vector Name: pET21_NESG               Format:  FUSION
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23447540
Title: The TP0796 lipoprotein of Treponema pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis
PMID: 23447540
Title: The TP0796 lipoprotein of Treponema pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis
Authors: PSI
Northeast Structural Genomics Consortium
EcCD00385238

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 996nts         Open reading frame : 13 to 1008

GGAGATATACATATGGATCAAAAACCACAACCCGCCAAAACCCACGCTACTGAAGTTACC
GTTCTTGAAGGCAAAACTATGGGTACCTTCTGGCGTGCCAGCATCCCGGGCATTGACGCC
AAACGCAGCGCCGAACTTAAAGAAAAGATTCAGACCCAACTGGACGCCGACGATCAGCTG
CTTTCGACCTATAAAAAAGATTCCGCGCTGATGCGCTTTAACGACTCGCAAAGTTTATCG
CCGTGGCCGGTAAGTGAAGCGATGGCCGATATCGTCACCACCTCGCTGCGCATTGGCGCG
AAGACCGATGGCGCGATGGATATAACCGTCGGGCCGCTGGTGAATCTGTGGGGCTTTGGC
CCGGAACAACAGCCGGTTCAAATTCCGAGCCAGGAACAGATCGATGCGATGAAAGCCAAA
ACCGGCTTACAGCACCTGACGGTCATCAATCAGTCGCATCAGCAATATCTGCAAAAAGAC
CTGCCGGATTTATATGTCGATCTCTCCACCGTCGGCGAAGGCTATGCGGCCGATCATTTG
GCACGCTTGATGGAGCAGGAAGGGATTTCCCGCTATCTGGTGTCGGTGGGCGGCGCGCTG
AACAGCCGTGGTATGAACGGTGAAGGCCTGCCGTGGCGGGTAGCGATTCAAAAACCAACC
GATAAAGAAAACGCGGTTCAGGCTGTGGTGGATATCAACGGTCATGGGATTAGCACCTCT
GGCAGCTACCGTAACTATTACGAACTGGACGGCAAACGTCTTTCCCATGTTATCGATCCG
CAAACCGGGCGTCCCATCGAACACAATCTGGTATCCGTGACGGTGATTGCTCCGACGGCG
CTGGAAGCCGATGCCTGGGATACTGGCTTGATGGTACTCGGGCCGGAGAAAGCTAAAGAG
GTTGTTCGCCGGGAAGGGCTGGCGGTTTATATGATCACCAAAGAAGGCGATAGCTTTAAA
ACCTGGATGTCACCACAGTTTAAAAGCTTCCTTGTCAGCGAAAAAAATCTCGAGCACCAC
CACCACCACCACTGAGATCCGG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAGGAGATATACAT

3' Linker Sequence:
CTCGAGCACCACCACCACCACCACTGA

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in ER559
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET21_NESG

pET21_NESG in advanced viewed

Synonyms: pET21, pET21-modified, pET21Cc, pET21_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5415
Parent Vector: None
Empty Vector: EvNO00336944
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Thomas B. Acton
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, BamHI, EcoRI, XhoI) 157 253
bacterial origin ori pBR322-type bacterial origin of replication 3180 3862
gene fragment ROP ROP 2580 2756
primer T7 forward T7 forward sequencing primer 283 298
primer T7 reverse T7 reverse sequencing primer 66 84
promoter T7 T7 promoter 280 299
repressor binding site lacO lacO regulatory sequence 258 280
repressor protein gene LacI LacI 752 1768
selectable marker AmpR ampicillin resistance gene 3960 4619
ssDNA origin f1 ori f1 origin 5081 5387
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 termination sequence 68 72

 

  • Protein
  • Clone
  • Experimental

Protein

PDB - Structural Annotation : 2O18
Structural Annotation Wiki : TOPSAN
UniProt : APBE_ECOLI
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 542305
HIP Master Clone ID : 452625
Original Clone ID : ER559-21.1

Experimental

Structural Biology Knowledgebase : SBKB (2O18)
TargetTrack - Experimental data : ER559