Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name:  None
Original Clone ID:
CpR63-21.1
Keyword:
None
Species:
Clostridium perfringens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
CpCD00385254
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1281nts
Open reading frame: 13 to 1293

GCGGGGGATCCGATGAGTTTATCAACTAACGAATTAAAAGAGATTGTCAGAAAAATTGGT
AAAGATTTATCAGGTAAAATAGAGGACAAGAAGTTACAAGAATTATTTTATAATTGTTTT
ATAAATACAATGGATACAACTGTAGAGGTTTCAGAGGGAGATGCCTTTGTAATTACTGGT
GATATTCCTGCAATGTGGCTTAGGGATTCCACTTCTCAAGTAGAGCATTACTTACCATTT
GTTAAGGAATATCCAGAATTAAAGGCTATTTTTACTGGATTAATAAATAGACAAGTAAAA
TGTATATTCATAGATCCATATGCAAATGCCTTTAATAAAGAGCCTAATGGACAAAAATGG
GACAATGATATAACTAAGGATAGCCCTTGGGTTTGGGAAAGAAAGTATGAAATAGATTCA
CTATGTTATCCAGTTAGATTAATACATAAGTATTGGAAAGAATCTGGTGATGAAACTTTC
TTTAATGATGATATTAAAAAAGCTTTCAATATGATAATAGATTTATGGAGAGTGGAGCAG
TATCATAGAGAAAAATCAGATTATAGCTTCCAAAGATTAAATTGTTCAGTAACTGACACT
TTAAGCCATGAAGGATTAGGAACTCCAGTTACTTACACAGGAATGACATGGTCAGGATTT
AGACCAAGTGATGATGCTTGTGAATATGGATACTTAATTCCAGCAAACATGTTCGCTGTA
GTTGCCTTAAGATATATTTCAGAAATTGCAGAGAAGGTTTATAAGGATGAAGAGCTTAAA
GAAAAAGCTGATAGTTTAAGAGAAGAAATAGATAATGCTATAGAAAAGCATGGAAAAGTT
TATAAAGAAGGATTTGGAGAAGTTTATGCATATGAAACTGATGGTATGGGTAACTATAAC
TTCATGGATGATGCTAATGTACCAAGCCTTCTATCAATTCCTTACTTAGAGTACAAAGGA
ATTGAAGATGAAGTTTATCAAAATACTAGAAAATTTATTTTGAGCAAAAATAATAGATTC
TTCTTTGAAGGAAAAGCTGCTAAAGGAATTGGAAGTCCACATACTCCAGACCAGTATATT
TGGCACATAGCTTTATCAATGCAAGGGTTAACAACAAATAATCAAGAGGAAATAGATCAG
TTAATTAAATTATTAAAAGAAACTGATGCAGGGACTGGATATATGCATGAAGGTTTCCAT
GTAGATGACCCTACTAAGTTTACAAGAGATTGGTTTGCATGGTCAAACTCATTATTCTCA
CACTTTATCTATGAGAAAGTTATAAATAAAAAA

Protein sequence predicted based on nucleotide sequence

MSLSTNELKEIVRKIGKDLSGKIEDKKLQELFYNCFINTMDTTVEVSEGDAFVITGDIPA
MWLRDSTSQVEHYLPFVKEYPELKAIFTGLINRQVKCIFIDPYANAFNKEPNGQKWDNDI
TKDSPWVWERKYEIDSLCYPVRLIHKYWKESGDETFFNDDIKKAFNMIIDLWRVEQYHRE
KSDYSFQRLNCSVTDTLSHEGLGTPVTYTGMTWSGFRPSDDACEYGYLIPANMFAVVALR
YISEIAEKVYKDEELKEKADSLREEIDNAIEKHGKVYKEGFGEVYAYETDGMGNYNFMDD
ANVPSLLSIPYLEYKGIEDEVYQNTRKFILSKNNRFFFEGKAAKGIGSPHTPDQYIWHIA
LSMQGLTTNNQEEIDQLIKLLKETDAGTGYMHEGFHVDDPTKFTRDWFAWSNSLFSHFIY
EKVINKK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAGGAGATATACATATGGCGGGGGATCCg
3' Linker Sequence:
CTCGAGCACCACCACCACCACCACTGA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in CpR63
Recommended expression in:
Not Applicable