TFAP2B (Homo sapiens) in pVP16

Explanation of Terms

Gene:
Gene Symbol:  TFAP2B
Gene Name:  transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
Original Clone ID:
1136.1.41_GO.37075
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00396714
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1368nts
Open reading frame: 51 to 1418

GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCCTCTGGAAGCTTGTGGAGAATGTCAAGTACGAAGATATCTATGAGGACC
GGCACGATGGTGTCCCGAGCCACAGCTCGCGGCTCTCCCAGCTGGGCTCAGTGTCCCAAG
GACCCTACTCGAGCGCCCCGCCGCTGTCCCACACCCCGTCGTCGGACTTCCAGCCGCCCT
ACTTCCCACCCCCCTACCAGCCGCTCCCCTACCACCAGAGCCAGGACCCCTACTCCCACG
TCAACGACCCCTACTCCCTGAACCCACTGCACCAGCCCCAGCAACATCCCTGGGGGCAAC
GGCAGCGGCAAGAAGTGGGTTCGGAAGCCGGCTCTCTCCTGCCCCAGCCTCGGGCCGCCT
TGCCCCAGCTCTCGGGCCTTGACCCCCGGAGGGACTACCACTCGGTCCGCCGGCCGGACG
TGCTGCTGCATTCGGCGCACCACGGCCTGGACGCGGGCATGGGTGACAGCCTCTCGCTGC
ACGGCCTCGGCCATCCCGGAATGGAAGACGTCCAGTCAGTTGAAGATGCCAATAACAGCG
GCATGAATCTATTGGACCAGTCTGTCATTAAAAAAGTTCCAGTTCCTCCCAAATCGGTGA
CTTCTCTAATGATGAATAAAGACGGCTTCCTGGGAGGCATGTCTGTCAACACCGGCGAGG
TGTTTTGCTCCGTCCCAGGCCGTTTGTCTCTGCTCAGTTCAACTTCGAAGTACAAAGTAA
CTGTGGGAGAAGTTCAGAGACGGCTGTCGCCCCCTGAATGCCTCAATGCATCTCTCCTCG
GCGGAGTCCTCAGAAGAGCCAAATCGAAAAATGGGGGGAGATCTTTGCGAGAAAGGCTAG
AAAAAATCGGTTTGAATTTACCCGCGGGCAGGCGCAAAGCAGCAAATGTCACGTTACTCA
CCTCCCTGGTGGAAGGAGAAGCTGTTCACTTAGCTAGGGATTTTGGGTACATTTGCGAAA
CGGAGTTTCCCGCCAAAGCCGTCTCTGAGTATTTGAACCGGCAGCACACAGACCCGAGTG
ACCTGCACTCCCGAAAGAATATGCTGTTGGCCACCAAGCAACTTTGTAAAGAATTTACGG
ATCTACTGGCGCAGGACCGGACACCGATAGGGAACAGCCGACCCAGCCCCATCCTGGAGC
CGGGGATCCAGAGCTGCCTCACGCACTTCAGCCTCATCACGCACGGCTTCGGCGCCCCGG
CCATTTGCGCCGCGCTCACGGCCCTGCAGAACTATCTCACCGAGGCGCTCAAAGGCATGG
ACAAGATGTTCTTGAACAACACCACCACTAACAGGCACACGTCTGGGGAAGGCCCAGGTA
GTAAAACTGGCGACAAGGAGGAGAAACACAGGAAATGATAGGACCCAGCTTTCTTGTACA
AAGTGGTGATGGCGCGCCTGTAGGACGTC

Protein sequence predicted based on nucleotide sequence

ENLYFQSLWKLVENVKYEDIYEDRHDGVPSHSSRLSQLGSVSQGPYSSAPPLSHTPSSDF
QPPYFPPPYQPLPYHQSQDPYSHVNDPYSLNPLHQPQQHPWGQRQRQEVGSEAGSLLPQP
RAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDA
NNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSK
YKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANV
TLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCK
EFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEAL
KGMDKMFLNNTTTNRHTSGEGPGSKTGDKEEKHRK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g120a,S40S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in GO.37075
Recommended expression in:
Not Applicable