SLC40A1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SLC40A1
Gene Name:  solute carrier family 40 (iron-regulated transporter), member 1
Original Clone ID:
FLH166738.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00039720
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1713nts
Open reading frame: 1 to 1713

ATGACCAGGGCGGGAGATCACAACCGCCAGAGAGGATGCTGTGGATCCTTGGCCGACTAC
CTGACCTCTGCAAAATTCCTTCTCTACCTTGGTCATTCTCTCTCTACTTGGGGAGATCGG
ATGTGGCACTTTGCGGTGTCTGTGTTTCTGGTAGAGCTCTATGGAAACAGCCTCCTTTTG
ACAGCAGTCTACGGGCTGGTGGTGGCAGGGTCTGTTCTGGTCCTGGGAGCCATCATCGGT
GACTGGGTGGACAAGAATGCTAGACTTAAAGTGGCCCAGACCTCGCTGGTGGTACAGAAT
GTTTCAGTCATCCTGTGTGGAATCATCCTGATGATGGTTTTCTTACATAAACATGAGCTT
CTGACCATGTACCATGGATGGGTTCTCACTTCCTGCTATATCCTGATCATCACTATTGCA
AATATTGCAAATTTGGCCAGTACTGCTACTGCAATCACAATCCAAAGGGATTGGATTGTT
GTTGTTGCAGGAGAAGACAGAAGCAAACTAGCAAATATGAATGCCACAATACGAAGGATT
GACCAGTTAACCAACATCTTAGCCCCCATGGCTGTTGGCCAGATTATGACATTTGGCTCC
CCAGTCATCGGCTGTGGCTTTATTTCGGGATGGAACTTGGTATCCATGTGCGTGGAGTAC
GTTCTGCTCTGGAAGGTTTACCAGAAAACCCCAGCTCTAGCTGTGAAAGCTGGTCTTAAA
GAAGAGGAAACTGAATTGAAACAGCTGAATTTACACAAAGATACTGAGCCAAAACCCCTG
GAGGGAACTCATCTAATGGGTGTGAAAGACTCTAACATCCATGAGCTTGAACATGAGCAA
GAGCCTACTTGTGCCTCCCAGATGGCTGAGCCCTTCCGTACCTTCCGAGATGGATGGGTC
TCCTACTACAACCAGCCTGTGTTTCTGGCTGGCATGGGTCTTGCTTTCCTTTATATGACT
GTCCTGGGCTTTGACTGCATCACCACAGGGTACGCCTACACTCAGGGACTGAGTGGTTCC
ATCCTCAGTATTTTGATGGGAGCATCAGCTATAACTGGAATAATGGGAACTGTAGCTTTT
ACTTGGCTACGTCGAAAATGTGGTTTGGTTCGGACAGGTCTGATCTCAGGATTGGCACAG
CTTTCCTGTTTGATCTTGTGTGTGATCTCTGTATTCATGCCTGGAAGCCCCCTGGACTTG
TCCGTTTCTCCTTTTGAAGATATCCGATCAAGGTTCATTCAAGGAGAGTCAATTACACCT
ACCAAGATACCTGAAATTACAACTGAAATATACATGTCTAATGGGTCTAATTCTGCTAAT
ATTGTCCCGGAGACAAGTCCTGAATCTGTGCCCATAATCTCTGTCAGTCTGCTGTTTGCA
GGCGTCATTGCTGCTAGAATCGGTCTTTGGTCCTTTGATTTAACTGTGACACAGTTGCTG
CAAGAAAATGTAATTGAATCTGAAAGAGGCATTATAAATGGTGTACAGAACTCCATGAAC
TATCTTCTTGATCTTCTGCATTTCATCATGGTCATCCTGGCTCCAAATCCTGAAGCTTTT
GGCTTGCTCGTATTGATTTCAGTCTCCTTTGTGGCAATGGGCCACATTATGTATTTCCGA
TTTGCCCAAAATACTCTGGGAAACAAGCTCTTTGCTTGCGGTCCTGATGCAAAAGAAGTT
AGGAAGGAAAATCAAGCAAATACATCTGTTGTT

Protein sequence predicted based on nucleotide sequence

MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL
TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHEL
LTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRI
DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLK
EEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWV
SYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAF
TWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITP
TKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLL
QENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFR
FAQNTLGNKLFACGPDAKEVRKENQANTSVV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2146
Start on reference sequence 431
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable