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RNASE9 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RNASE9
Gene Name:  ribonuclease, RNase A family, 9 (non-active)
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072777)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00398707

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 615nts         Open reading frame : 1 to 615
Reference Sequence Alignment

ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTATTGCCGCAGCAGCTG
CTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTCCCAGAAGAAGATAAA
AAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGGCCCGCCAGACCACCT
ACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATGCCACTAAATCATATA
GAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAACACCGTTGGGTGGCA
GAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATCTGTTACAACAGATTT
GTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGTCTTGTAGAAGGAGTG
TATTGTAATTTAACAGAAGCATCTGAAATACCAGCGTGTAAATACGAATCACTTTATAGG
AAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAAAAACGTATTCCTCAT
ACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTCAGTGAGGATGGTGTC
TTTGTCATACCGCCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNASE9
Symbol Nomenclature : RNASE9
SYNONYM : HEL128; RAK1; h461
Designation : inactive ribonuclease-like protein 9|ribonuclear enzyme|ribonuclease A K1
Full Nomenclature : ribonuclease, RNase A family, 9 (non-active)
GENEID : 390443
GenBank Accession : HQ258747
HGNC : 20673
MIM : 614014
Vega : OTTHUMG00000170989
Target GenBank: HQ258747

 

Reference Sequence Alignment

  

HsCD00398707        ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110359.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001289110.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110356.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001001673.3      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110361.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110357.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110360.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110358.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
                                   *********************************************

HsCD00398707        TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110359.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001289110.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110356.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001001673.3      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110361.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110357.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110360.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110358.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
                    ************************************************************

HsCD00398707        CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110359.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001289110.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110356.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001001673.3      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110361.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110357.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110360.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110358.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
                    ************************************************************

HsCD00398707        CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110359.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001289110.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110356.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001001673.3      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110361.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110357.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110360.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110358.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
                    ************************************************************

HsCD00398707        CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110359.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001289110.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110356.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001001673.3      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110361.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110357.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110360.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110358.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
                    ************************************************************

HsCD00398707        CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110359.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001289110.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110356.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001001673.3      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110361.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110357.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110360.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110358.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
                    ************************************************************

HsCD00398707        TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110359.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001289110.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110356.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001001673.3      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110361.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110357.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110360.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110358.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
                    ************************************************************

HsCD00398707        CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATCTGAAATACCAGCGTGTAAATAC
NM_001110359.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001289110.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110356.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001001673.3      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110361.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110357.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110360.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110358.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
                    ************************************* **********************

HsCD00398707        GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110359.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001289110.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110356.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001001673.3      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110361.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110357.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110360.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110358.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
                    ************************************************************

HsCD00398707        AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110359.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001289110.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110356.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001001673.3      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110361.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110357.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110360.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110358.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
                    ************************************************************

HsCD00398707        AGTGAGGATGGTGTCTTTGTCATACCGCCC---
NM_001110359.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001289110.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110356.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001001673.3      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110361.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110357.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110360.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110358.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
                    ************************ *****   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 17983

Clone

Cloning Information : 571245

Organism


TAX_ID : 9606
Species Specific ID: 390443

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000188655

Reagents

Labome : RNASE9-antibody