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CAPN11 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN11
Gene Name:  calpain 11
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100073459)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00399267

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2106nts         Open reading frame : 51 to 2156
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGTGGCTC
ACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAACT
TTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTCG
AGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAACT
CCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTAT
TCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGGC
TGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCCA
GAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTTG
GACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTGT
TTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCCA
AGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGACT
TCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGGC
TCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGTG
ATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTGA
CTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAATC
CCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAGG
TGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATGT
CCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGATA
CACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGCA
GAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAGT
TTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTGG
CCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGAG
CCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATTC
AGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAGA
TCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATCA
TTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACCG
AGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAGG
AAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCAG
GAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATCA
AATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAACC
TCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAAA
AACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTGA
ACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTAA
TGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTCA
TCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAGA
ATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATACC
CAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN11
Symbol Nomenclature : CAPN11
SYNONYM : calpain11
Designation : CANP 11|calcium-activated neutral proteinase 11|calpain-11
Full Nomenclature : calpain 11
GENEID : 11131
GenBank Accession : JF432283
HGNC : 1478
MIM : 604822
Vega : OTTHUMG00000014758
Target GenBank: JF432283

 

Reference Sequence Alignment

  

HsCD00399267      ------------------------------------------------------------
NM_007058.3       ATGCTGTACTCCCCAGGGCCGAGTCTTCCGGAGTCAGCAGAGAGCCTGGATGGATCACAG
                                                                              

HsCD00399267      -TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGTGGCT
NM_007058.3       GAGGATAAGCCTCGGGGCTCATGTGCGGAGCCCACTTTTACTGATACGGGAATGGTGGCT
                   :  **:**  : *    * :: :. * ...* :  *: *.:.*:.  **.*********

HsCD00399267      CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
NM_007058.3       CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
                  ************************************************************

HsCD00399267      TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
NM_007058.3       TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
                  ************************************************************

HsCD00399267      GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
NM_007058.3       GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
                  ************************************************************

HsCD00399267      TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
NM_007058.3       TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
                  ************************************************************

HsCD00399267      TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
NM_007058.3       TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
                  ************************************************************

HsCD00399267      CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
NM_007058.3       CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
                  ************************************************************

HsCD00399267      AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
NM_007058.3       AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
                  ************************************************************

HsCD00399267      GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
NM_007058.3       GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
                  ************************************************************

HsCD00399267      TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
NM_007058.3       TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
                  ************************************************************

HsCD00399267      AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
NM_007058.3       AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
                  ************************************************************

HsCD00399267      TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
NM_007058.3       TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
                  ************************************************************

HsCD00399267      CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
NM_007058.3       CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
                  ************************************************************

HsCD00399267      GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
NM_007058.3       GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
                  ************************************************************

HsCD00399267      ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
NM_007058.3       ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
                  ************************************************************

HsCD00399267      CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
NM_007058.3       CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
                  ************************************************************

HsCD00399267      GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
NM_007058.3       GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
                  ************************************************************

HsCD00399267      TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
NM_007058.3       TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
                  ************************************************************

HsCD00399267      ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
NM_007058.3       ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
                  ************************************************************

HsCD00399267      AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
NM_007058.3       AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
                  ************************************************************

HsCD00399267      TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
NM_007058.3       TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
                  ************************************************************

HsCD00399267      GCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
NM_007058.3       GTCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
                  * **********************************************************

HsCD00399267      GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
NM_007058.3       GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
                  ************************************************************

HsCD00399267      CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
NM_007058.3       CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
                  ************************************************************

HsCD00399267      ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
NM_007058.3       ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
                  ************************************************************

HsCD00399267      ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
NM_007058.3       ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
                  ************************************************************

HsCD00399267      GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
NM_007058.3       GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
                  ************************************************************

HsCD00399267      GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
NM_007058.3       GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
                  ************************************************************

HsCD00399267      GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
NM_007058.3       GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
                  ************************************************************

HsCD00399267      AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
NM_007058.3       AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
                  ************************************************************

HsCD00399267      CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
NM_007058.3       CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
                  ************************************************************

HsCD00399267      AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
NM_007058.3       AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
                  ************************************************************

HsCD00399267      AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
NM_007058.3       AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
                  ************************************************************

HsCD00399267      ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
NM_007058.3       ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
                  ************************************************************

HsCD00399267      ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
NM_007058.3       ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
                  ************************************************************

HsCD00399267      AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAC
NM_007058.3       AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAG
                  *********************************************************** 

HsCD00399267      CCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_007058.3       -------------------------------------------------
                                                                   

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : calpain_III :
SMART domain : CysPc : Calpain-like thiol protease family.
HPRD : 05315

Clone

Cloning Information : 573365

Organism


TAX_ID : 9606
Species Specific ID: 11131

Genome

Chromosome : 6
Map Location : 6p12
Ensembl : ENSG00000137225

Reagents

Labome : CAPN11-antibody