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KLHL29 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL29
Gene Name:  kelch-like family member 29
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100073337)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00399449

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1509nts         Open reading frame : 51 to 1559
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGCCAGGCC
ACTACTCGCTCCCTCAGCCGCCCTCTCAGCCACTGAGCAGCGTGGTGGTCAACATGCCTG
CCCAGGCCCTGTATGCCAGCCCTCAGCCCCTGGCCGTGTCCACACTGCCCGGTGTGGGGC
AGGTGGCCCGCCCAGGACCCACCGCTGTGGGCAACGGCCACATGGCAGGGCCCCTGCTGC
CTCCACCGCCGCCAGCCCAGCCGTCCGCCACTCTCCCCAGTGGTGCCCCTGCCACCAATG
GGCCCCCCACAACCGACTCGGCCCACGGGCTGCAGATGCTGCGGACCATTGGCGTGGGGA
AGTATGAGTTCACCGACCCGGGGCACCCCAGAGAAATGTTGAAGGAATTGAACCAGCAAC
GCAGAGCGAAAGCGTTTACAGACCTGAAAATTGTTGTTGAAGGCAGAGAGTTTGAAGTCC
ACCAAAATGTTCTAGCTTCCTGCAGCTTGTATTTCAAGGACCTGATTCAAAGGTCCGTGC
AAGACAGCGGCCAGGGCGGCCGGGAGAAGCTGGAGCTCGTCCTGTCGAACCTGCAGGCAG
ACGTCCTGGAGTTGCTGCTGGAGTTTGTCTACACGGGCTCCCTGGTCATCGACTCGGCCA
ACGCCAAGACACTGCTGGAGGCGGCCAGCAAGTTCCAGTTCCACACCTTCTGCAAAGTCT
GCGTGTCCTTTCTCGAGAAGCAGCTGACGGCCAGCAACTGCCTGGGCGTGCTGGCCATGG
CCGAGGCCATGCAGTGCAGCGAGCTCTACCACATGGCCAAGGCCTTCGCGCTGCAGATCT
TCCCCGAGGTGGCCGCCCAGGAGGAGATCCTCAGCATCTCCAAGGACGACTTCATCGCCT
ACGTCTCCAACGACAGCCTCAACACCAAGGCTGAGGAGCTGGTGTACGAGACAGTCATCA
AGTGGATCAAGAAGGACCCCGCGACACGCACACAGTACGCGGCTGAGCTCCTGGCCGTGG
TCCGCCTCCCCTTCATCCACCCCAGCTACCTGCTCAATGTGGTTGACAATGAAGAGCTGA
TCAAGTCATCAGAAGCCTGCCGGGACCTGGTGAACGAGGCCAAACGCTACCATATGCTGC
CCCACGCCCGCCAGGAGATGCAGACGCCCCGAACCCGGCCGCGCCTCTCTGCAGGTGTGG
CTGAGGTCATCGTCTTGGTTGGGGGCCGTCAGATGGTGGGGATGACCCAGCGCTCGCTGG
TGGCCGTCACCTGCTGGAACCCGCAGAACAACAAGTGGTACCCCTTGGCCTCGCTGCCCT
TCTATGACCGCGAGTTCTTCAGTGTAGTGAGTGCAGGGGACAACATCTACCTCTCAGGTG
GGATGGAATCAGGGGTGACGCTGGCTGATGTCTGGTGCTACATGTCCCTGCTTGATAACT
GGAACCTCGTCTCCAGAATGACAGTCCCCCGCTGTCGGCACAATAGCCTCGTCTACGATG
GGAAGATTTACACCCTCGGGGGACTTGGCGTGGCAGGCAACGTGGACCATGTGGAGAGGT
ACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL29
Symbol Nomenclature : KLHL29
SYNONYM : KBTBD9
Designation : kelch repeat and BTB (POZ) domain containing 9|kelch repeat and BTB domain-containing protein 9|kelch-like 29|kelch-like protein 29
Full Nomenclature : kelch-like family member 29
GENEID : 114818
GenBank Accession : JF432182
HGNC : 29404
Vega : OTTHUMG00000151899
Target GenBank: JF432182

 

Reference Sequence Alignment

  

HsCD00399449      ------------------------------------------------------------
NM_052920.1       ATGTCCCGGCACCATAGCCGCTTCGAAAGAGATTACCGGGTGGGCTGGGACCGCCGCGAA
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       TGGAGCGTCAACGGGACGCATGGGACCACCAGCATCTGCAGTGTCACCTCGGGGGCCGGT
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       GGCGGCACAGCCAGCAGCCTCAGCGTCCGGCCCGGCCTCCTGCCGCTGCCCGTGGTGCCC
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       TCCCGGCTGCCCACCCCGGCTACAGCTCCTGCTCCCTGCACCACCGGCAGCAGCGAGGCC
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       ATCACCAGCCTCGTGGCCAGCTCTGCGTCTGCGGTCACCACCAAGGCTCCCGGCATCTCC
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       AAAGGGGACAGTCAGTCCCAGGGACTGGCGACCAGCATCCGGTGGGGGCAGACGCCTATC
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       AATCAGTCCACACCCTGGGACACTGATGAGCCACCCTCCAAACAGATGAGAGAGAGTGAC
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       AATCCAGGCACAGGGCCATGGGTGACCACGGTGGCCGCCGGGAACCAGCCCACCCTGATT
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       GCACACTCCTATGGAGTGGCCCAGCCTCCCACCTTCAGCCCGGCTGTGAACGTCCAGGCC
                                                                              

HsCD00399449      -------TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATG
NM_052920.1       CCGGTCATTGGGGTGACCCCCTCACTGCCTCCCCACGTGGGGCCCCAGCTCCCGCTGATG
                         *  .  :*. .  ** : * . :.  *...    * .*.*..:.      ***

HsCD00399449      CCAGGCCACTACTCGCTCCCTCAGCCGCCCTCTCAGCCACTGAGCAGCGTGGTGGTCAAC
NM_052920.1       CCAGGCCACTACTCGCTCCCTCAGCCGCCCTCTCAGCCACTGAGCAGCGTGGTGGTCAAC
                  ************************************************************

HsCD00399449      ATGCCTGCCCAGGCCCTGTATGCCAGCCCTCAGCCCCTGGCCGTGTCCACACTGCCCGGT
NM_052920.1       ATGCCTGCCCAGGCCCTGTATGCCAGCCCTCAGCCCCTGGCCGTGTCCACACTGCCCGGT
                  ************************************************************

HsCD00399449      GTGGGGCAGGTGGCCCGCCCAGGACCCACCGCTGTGGGCAACGGCCACATGGCAGGGCCC
NM_052920.1       GTGGGGCAGGTGGCCCGCCCAGGACCCACCGCTGTGGGCAACGGCCACATGGCAGGGCCC
                  ************************************************************

HsCD00399449      CTGCTGCCTCCACCGCCGCCAGCCCAGCCGTCCGCCACTCTCCCCAGTGGTGCCCCTGCC
NM_052920.1       CTGCTGCCTCCACCGCCGCCAGCCCAGCCGTCCGCCACTCTCCCCAGTGGTGCCCCTGCC
                  ************************************************************

HsCD00399449      ACCAATGGGCCCCCCACAACCGACTCGGCCCACGGGCTGCAGATGCTGCGGACCATTGGC
NM_052920.1       ACCAATGGGCCCCCCACAACCGACTCGGCCCACGGGCTGCAGATGCTGCGGACCATTGGC
                  ************************************************************

HsCD00399449      GTGGGGAAGTATGAGTTCACCGACCCGGGGCACCCCAGAGAAATGTTGAAGGAATTGAAC
NM_052920.1       GTGGGGAAGTATGAGTTCACCGACCCGGGGCACCCCAGAGAAATGTTGAAGGAATTGAAC
                  ************************************************************

HsCD00399449      CAGCAACGCAGAGCGAAAGCGTTTACAGACCTGAAAATTGTTGTTGAAGGCAGAGAGTTT
NM_052920.1       CAGCAACGCAGAGCGAAAGCGTTTACAGACCTGAAAATTGTTGTTGAAGGCAGAGAGTTT
                  ************************************************************

HsCD00399449      GAAGTCCACCAAAATGTTCTAGCTTCCTGCAGCTTGTATTTCAAGGACCTGATTCAAAGG
NM_052920.1       GAAGTCCACCAAAATGTTCTAGCTTCCTGCAGCTTGTATTTCAAGGACCTGATTCAAAGG
                  ************************************************************

HsCD00399449      TCCGTGCAAGACAGCGGCCAGGGCGGCCGGGAGAAGCTGGAGCTCGTCCTGTCGAACCTG
NM_052920.1       TCCGTGCAAGACAGCGGCCAGGGCGGCCGGGAGAAGCTGGAGCTCGTCCTGTCGAACCTG
                  ************************************************************

HsCD00399449      CAGGCAGACGTCCTGGAGTTGCTGCTGGAGTTTGTCTACACGGGCTCCCTGGTCATCGAC
NM_052920.1       CAGGCAGACGTCCTGGAGTTGCTGCTGGAGTTTGTCTACACGGGCTCCCTGGTCATCGAC
                  ************************************************************

HsCD00399449      TCGGCCAACGCCAAGACACTGCTGGAGGCGGCCAGCAAGTTCCAGTTCCACACCTTCTGC
NM_052920.1       TCGGCCAACGCCAAGACACTGCTGGAGGCGGCCAGCAAGTTCCAGTTCCACACCTTCTGC
                  ************************************************************

HsCD00399449      AAAGTCTGCGTGTCCTTTCTCGAGAAGCAGCTGACGGCCAGCAACTGCCTGGGCGTGCTG
NM_052920.1       AAAGTCTGCGTGTCCTTTCTCGAGAAGCAGCTGACGGCCAGCAACTGCCTGGGCGTGCTG
                  ************************************************************

HsCD00399449      GCCATGGCCGAGGCCATGCAGTGCAGCGAGCTCTACCACATGGCCAAGGCCTTCGCGCTG
NM_052920.1       GCCATGGCCGAGGCCATGCAGTGCAGCGAGCTCTACCACATGGCCAAGGCCTTCGCGCTG
                  ************************************************************

HsCD00399449      CAGATCTTCCCCGAGGTGGCCGCCCAGGAGGAGATCCTCAGCATCTCCAAGGACGACTTC
NM_052920.1       CAGATCTTCCCCGAGGTGGCCGCCCAGGAGGAGATCCTCAGCATCTCCAAGGACGACTTC
                  ************************************************************

HsCD00399449      ATCGCCTACGTCTCCAACGACAGCCTCAACACCAAGGCTGAGGAGCTGGTGTACGAGACA
NM_052920.1       ATCGCCTACGTCTCCAACGACAGCCTCAACACCAAGGCTGAGGAGCTGGTGTACGAGACA
                  ************************************************************

HsCD00399449      GTCATCAAGTGGATCAAGAAGGACCCCGCGACACGCACACAGTACGCGGCTGAGCTCCTG
NM_052920.1       GTCATCAAGTGGATCAAGAAGGACCCCGCGACACGCACACAGTACGCGGCTGAGCTCCTG
                  ************************************************************

HsCD00399449      GCCGTGGTCCGCCTCCCCTTCATCCACCCCAGCTACCTGCTCAATGTGGTTGACAATGAA
NM_052920.1       GCCGTGGTCCGCCTCCCCTTCATCCACCCCAGCTACCTGCTCAATGTGGTTGACAATGAA
                  ************************************************************

HsCD00399449      GAGCTGATCAAGTCATCAGAAGCCTGCCGGGACCTGGTGAACGAGGCCAAACGCTACCAT
NM_052920.1       GAGCTGATCAAGTCATCAGAAGCCTGCCGGGACCTGGTGAACGAGGCCAAACGCTACCAT
                  ************************************************************

HsCD00399449      ATGCTGCCCCACGCCCGCCAGGAGATGCAGACGCCCCGAACCCGGCCGCGCCTCTCTGCA
NM_052920.1       ATGCTGCCCCACGCCCGCCAGGAGATGCAGACGCCCCGAACCCGGCCGCGCCTCTCTGCA
                  ************************************************************

HsCD00399449      GGTGTGGCTGAGGTCATCGTCTTGGTTGGGGGCCGTCAGATGGTGGGGATGACCCAGCGC
NM_052920.1       GGTGTGGCTGAGGTCATCGTCTTGGTTGGGGGCCGTCAGATGGTGGGGATGACCCAGCGC
                  ************************************************************

HsCD00399449      TCGCTGGTGGCCGTCACCTGCTGGAACCCGCAGAACAACAAGTGGTACCCCTTGGCCTCG
NM_052920.1       TCGCTGGTGGCCGTCACCTGCTGGAACCCGCAGAACAACAAGTGGTACCCCTTGGCCTCG
                  ************************************************************

HsCD00399449      CTGCCCTTCTATGACCGCGAGTTCTTCAGTGTAGTGAGTGCAGGGGACAACATCTACCTC
NM_052920.1       CTGCCCTTCTATGACCGCGAGTTCTTCAGTGTAGTGAGTGCAGGGGACAACATCTACCTC
                  ************************************************************

HsCD00399449      TCAGGTGGGATGGAATCAGGGGTGACGCTGGCTGATGTCTGGTGCTACATGTCCCTGCTT
NM_052920.1       TCAGGTGGGATGGAATCAGGGGTGACGCTGGCTGATGTCTGGTGCTACATGTCCCTGCTT
                  ************************************************************

HsCD00399449      GATAACTGGAACCTCGTCTCCAGAATGACAGTCCCCCGCTGTCGGCACAATAGCCTCGTC
NM_052920.1       GATAACTGGAACCTCGTCTCCAGAATGACAGTCCCCCGCTGTCGGCACAATAGCCTCGTC
                  ************************************************************

HsCD00399449      TACGATGGGAAGATTTACACCCTCGGGGGACTTGGCGTGGCAGGCAACGTGGACCATGTG
NM_052920.1       TACGATGGGAAGATTTACACCCTCGGGGGACTTGGCGTGGCAGGCAACGTGGACCACGTG
                  ******************************************************** ***

HsCD00399449      GAGAGGTACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA--
NM_052920.1       GAGAGGTACGACACCATCACCAACCAATGGGAGGCGGTGGCCCCTCTGCCCAAGGCAGTA
                  ********* ..** :**:  :**.**   *. .  .*.. ... *:   *::. **.  

HsCD00399449      ------------------------------------------------------------
NM_052920.1       CACTCTGCTGCAGCCACAGTGTGTGGCGGCAAGATCTACGTGTTTGGTGGGGTGAACGAG
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       GCAGGCCGAGCTGCCGGCGTCCTCCAGTCTTACGTTCCTCAGACCAACACGTGGAGCTTC
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       ATCGAGTCCCCAATGATTGACAACAAGTATGCCCCCGCTGTCACGCTCAATGGCTTCGTT
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       TTCATCCTGGGCGGGGCTTATGCCAGAGCTACCACCATCTACGACCCTGAGAAAGGAAAC
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       ATTAAGGCGGGCCCAAACATGAACCACTCTCGCCAGTTCTGCAGTGCTGTGGTGCTTGAT
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       GGCAAGATTTATGCAACTGGAGGTATTGTCAGCAGTGAAGGGCCCGCGCTGGGCAACATG
                                                                              

HsCD00399449      ------------------------------------------------------------
NM_052920.1       GAGGCCTACGAGCCCACAACCAACACATGGACCCTCCTCCCCCACATGCCCTGCCCTGTG
                                                                              

HsCD00399449      ------------------------------------------------
NM_052920.1       TTCAGACACGGCTGCGTCGTGATAAAGAAATATATTCAAAGCGGCTGA
                                                                  

Clone

Cloning Information : 573547

Organism


TAX_ID : 9606
Species Specific ID: 114818

Genome

Chromosome : 2
Map Location : 2p24.1
Ensembl : ENSG00000119771

Reagents

Labome : KLHL29-antibody