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GABRR2 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GABRR2
Gene Name:  gamma-aminobutyric acid (GABA) A receptor, rho 2
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100072868)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00403505

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1395nts         Open reading frame : 1 to 1395
Reference Sequence Alignment

ATGCCTTATTTTACAAGACTCATTTTGTTCTTGTTTTGCTTGATGGTTCTCGTGGAGAGC
AGAAAACCCAAGAGGAAGCGATGGACAGGGCAGGTGGAAATGCCCAAGCCAAGTCACTTA
TATAAGAAGAACCTTGATGTGACCAAGATCCGGAAGGGAAAGCCTCAGCAGCTTCTCAGA
GTGGACGAGCACGACTTCAGCATGAGACCCGCCTTCGGAGGCCCTGCCATCCCGGTGGGC
GTGGACGTACAGGTGGAGAGCCTGGACAGCATCTCCGAGGTGGACATGGACTTCACTATG
ACCCTGTACCTGCGGCATTACTGGAAGGATGAGAGGCTAGCTTTCTCCAGCGCCAGCAAC
AAGAGCATGACCTTCGATGGCCGGCTGGTGAAGAAGATCTGGGTCCCTGATGTCTTCTTT
GTTCACTCCAAAAGATCGTTCACTCATGACACCACCACTGACAACATCATGCTGAGGGTG
TTCCCAGATGGACACGTGCTGTACAGCATGAGGATTACGGTCACTGCCATGTGCAACATG
GACTTCAGCCACTTTCCCCTGGACTCCCAGACCTGTTCTTTGGAGCTGGAGAGCTATGCC
TATACAGATGAAGATCTAATGCTGTACTGGAAGAATGGGGATGAATCCCTAAAAACAGAT
GAGAAGATCTCCTTGTCTCAGTTTCTGATTCAGAAATTTCACACAACTTCCAGGCTGGCC
TTCTACAGCAGCACTGGCTGGTACAACCGTCTGTACATTAACTTCACGTTGCGTCGCCAC
ATCTTCTTCTTCTTGCTCCAAACATATTTCCCTGCCACTCTGATGGTCATGCTGTCCTGG
GTGTCCTTCTGGATCGACCGCAGAGCTGTGCCTGCCAGAGTTTCACTGGGTATCACGACG
GTGCTGACCATGACCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTAC
GTCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTG
GAGTATGCGGCTGTCAACTACCTGACCACCGTGCAGGAGCGCAAGGAACGGAAGCTGCGG
GAGAAGTTCCCGTGCATGTGTGGAATGCTTCATTCAAAAACCATGATGCTGGATGGAAGC
TACAGTGAGTCTGAGGCCAACAGCCTGGCTGGGTACCCCAGAAGCCATATCCTGACAGAA
GAAGAAAGGCAAGACAAAATAGTGGTCCACCTGGGCCTGAGTGGTGAAGCCAACGCTGCC
AGAAAGAAGGGGCTTCTGAAGGGCCAGACGGGTTTTCGTATCTTCCAGAATACCCATGCC
ATTGACAAATACTCTAGGTTGATATTCCCTGCCTCCTACATATTTTTCAACTTAATTTAT
TGGTCAGTGTTTTCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GABRR2
Symbol Nomenclature : GABRR2
Designation : GABA(A) receptor subunit rho-2|GABA(A) receptor, rho 2|GABA(C) receptor|GABA-C receptor, rho-2 subunit|gamma-aminobutyric acid (GABA) receptor, rho 2|gamma-aminobutyric acid receptor subunit rho-2
Full Nomenclature : gamma-aminobutyric acid (GABA) A receptor, rho 2
GENEID : 2570
Locus Tag : RP1-131H7.1
GenBank Accession : HQ258439
HGNC : 4091
MIM : 137162
Vega : OTTHUMG00000015198
Target GenBank: HQ258439

 

Reference Sequence Alignment

  

HsCD00403505      ATGCCTTATTTTACAAGACTCATTTTGTTCTTGTTTTGCTTGATGGTTCTCGTGGAGAGC
NM_002043.3       ATGCCTTATTTTACAAGACTCATTTTGTTCTTGTTTTGCTTGATGGTTCTCGTGGAGAGC
                  ************************************************************

HsCD00403505      AGAAAACCCAAGAGGAAGCGATGGACAGGGCAGGTGGAAATGCCCAAGCCAAGTCACTTA
NM_002043.3       AGAAAACCCAAGAGGAAGCGATGGACAGGGCAGGTGGAAATGCCCAAGCCAAGTCACTTA
                  ************************************************************

HsCD00403505      TATAAGAAGAACCTTGATGTGACCAAGATCCGGAAGGGAAAGCCTCAGCAGCTTCTCAGA
NM_002043.3       TATAAGAAGAACCTTGATGTGACCAAGATCCGGAAGGGAAAGCCTCAGCAGCTTCTCAGA
                  ************************************************************

HsCD00403505      GTGGACGAGCACGACTTCAGCATGAGACCCGCCTTCGGAGGCCCTGCCATCCCGGTGGGC
NM_002043.3       GTGGACGAGCACGACTTCAGCATGAGACCCGCCTTCGGAGGCCCTGCCATCCCGGTGGGC
                  ************************************************************

HsCD00403505      GTGGACGTACAGGTGGAGAGCCTGGACAGCATCTCCGAGGTGGACATGGACTTCACTATG
NM_002043.3       GTGGACGTACAGGTGGAGAGCCTGGACAGCATCTCCGAGGTGGACATGGACTTCACTATG
                  ************************************************************

HsCD00403505      ACCCTGTACCTGCGGCATTACTGGAAGGATGAGAGGCTAGCTTTCTCCAGCGCCAGCAAC
NM_002043.3       ACCCTGTACCTGCGGCATTACTGGAAGGATGAGAGGCTAGCTTTCTCCAGCGCCAGCAAC
                  ************************************************************

HsCD00403505      AAGAGCATGACCTTCGATGGCCGGCTGGTGAAGAAGATCTGGGTCCCTGATGTCTTCTTT
NM_002043.3       AAGAGCATGACCTTCGATGGCCGGCTGGTGAAGAAGATCTGGGTCCCTGATGTCTTCTTT
                  ************************************************************

HsCD00403505      GTTCACTCCAAAAGATCGTTCACTCATGACACCACCACTGACAACATCATGCTGAGGGTG
NM_002043.3       GTTCACTCCAAAAGATCGTTCACTCATGACACCACCACTGACAACATCATGCTGAGGGTG
                  ************************************************************

HsCD00403505      TTCCCAGATGGACACGTGCTGTACAGCATGAGGATTACGGTCACTGCCATGTGCAACATG
NM_002043.3       TTCCCAGATGGACACGTGCTGTACAGCATGAGGATTACGGTCACTGCCATGTGCAACATG
                  ************************************************************

HsCD00403505      GACTTCAGCCACTTTCCCCTGGACTCCCAGACCTGTTCTTTGGAGCTGGAGAGCTATGCC
NM_002043.3       GACTTCAGCCACTTTCCCCTGGACTCCCAGACCTGTTCTTTGGAGCTGGAGAGCTATGCC
                  ************************************************************

HsCD00403505      TATACAGATGAAGATCTAATGCTGTACTGGAAGAATGGGGATGAATCCCTAAAAACAGAT
NM_002043.3       TATACAGATGAAGATCTAATGCTGTACTGGAAGAATGGGGATGAATCCCTAAAAACAGAT
                  ************************************************************

HsCD00403505      GAGAAGATCTCCTTGTCTCAGTTTCTGATTCAGAAATTTCACACAACTTCCAGGCTGGCC
NM_002043.3       GAGAAGATCTCCTTGTCTCAGTTTCTGATTCAGAAATTTCACACAACTTCCAGGCTGGCC
                  ************************************************************

HsCD00403505      TTCTACAGCAGCACTGGCTGGTACAACCGTCTGTACATTAACTTCACGTTGCGTCGCCAC
NM_002043.3       TTCTACAGCAGCACTGGCTGGTACAACCGTCTGTACATTAACTTCACGTTGCGTCGCCAC
                  ************************************************************

HsCD00403505      ATCTTCTTCTTCTTGCTCCAAACATATTTCCCTGCCACTCTGATGGTCATGCTGTCCTGG
NM_002043.3       ATCTTCTTCTTCTTGCTCCAAACATATTTCCCTGCCACTCTGATGGTCATGCTGTCCTGG
                  ************************************************************

HsCD00403505      GTGTCCTTCTGGATCGACCGCAGAGCTGTGCCTGCCAGAGTTTCACTGGGTATCACGACG
NM_002043.3       GTGTCCTTCTGGATCGACCGCAGAGCTGTGCCTGCCAGAGTTTCACTGGGTATCACGACG
                  ************************************************************

HsCD00403505      GTGCTGACCATGACCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTAC
NM_002043.3       GTGCTGACCATGACCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTAC
                  ************************************************************

HsCD00403505      GTCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTG
NM_002043.3       GTCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTG
                  ************************************************************

HsCD00403505      GAGTATGCGGCTGTCAACTACCTGACCACCGTGCAGGAGCGCAAGGAACGGAAGCTGCGG
NM_002043.3       GAGTATGCGGCTGTCAACTACCTGACCACCGTGCAGGAGCGCAAGGAACGGAAGCTGCGG
                  ************************************************************

HsCD00403505      GAGAAGTTCCCGTGCATGTGTGGAATGCTTCATTCAAAAACCATGATGCTGGATGGAAGC
NM_002043.3       GAGAAGTTCCCGTGCATGTGTGGAATGCTTCATTCAAAAACCATGATGCTGGATGGAAGC
                  ************************************************************

HsCD00403505      TACAGTGAGTCTGAGGCCAACAGCCTGGCTGGGTACCCCAGAAGCCATATCCTGACAGAA
NM_002043.3       TACAGTGAGTCTGAGGCCAACAGCCTGGCTGGGTACCCCAGAAGCCATATCCTGACAGAA
                  ************************************************************

HsCD00403505      GAAGAAAGGCAAGACAAAATAGTGGTCCACCTGGGCCTGAGTGGTGAAGCCAACGCTGCC
NM_002043.3       GAAGAAAGGCAAGACAAAATAGTGGTCCACCTGGGCCTGAGTGGTGAAGCCAACGCTGCC
                  ************************************************************

HsCD00403505      AGAAAGAAGGGGCTTCTGAAGGGCCAGACGGGTTTTCGTATCTTCCAGAATACCCATGCC
NM_002043.3       AGAAAGAAGGGGCTTCTGAAGGGCCAGACGGGTTTTCGTATCTTCCAGAATACCCATGCC
                  ************************************************************

HsCD00403505      ATTGACAAATACTCTAGGTTGATATTCCCTGCCTCCTACATATTTTTCAACTTAATTTAT
NM_002043.3       ATTGACAAATACTCTAGGTTGATATTCCCTGCCTCCTACATATTTTTCAACTTAATTTAT
                  ************************************************************

HsCD00403505      TGGTCAGTGTTTTCC---
NM_002043.3       TGGTCAGTGTTTTCCTAG
                  ***************   

Pathways

Reactome : GABA A (rho) receptor activation
Reactome : GABA receptor activation
Reactome : Ion channel transport
Reactome : Ligand-gated ion channel transport
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Transmembrane transport of small molecules
Reactome : Transmission across Chemical Synapses

Protein

HPRD : 15922

Clone

Cloning Information : 574209

Organism


TAX_ID : 9606
Species Specific ID: 2570

Genome

Chromosome : 6
Map Location : 6q15
Ensembl : ENSG00000111886

Reagents

Labome : GABRR2-antibody