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MRPL45 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MRPL45
Gene Name:  mitochondrial ribosomal protein L45
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100072794)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00403746

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 918nts         Open reading frame : 1 to 918
Reference Sequence Alignment

ATGGCAGCCCCCATACCTCAAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTT
CGGCAGCCAGTTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGT
TTCACACCTCCTATTTATCAACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCC
CGGAAAGCAGGATTGGTTATTCCTCCAGAAAAATCGGACCGTTCCATACATCTGGCCTGT
ACAGCTGGTATATTTGATGCCTATGTTCCTCCTGAGGGTGATGCACGCATATCATCTCTT
TCAAAGGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGCATCACAAGTG
TCAATCCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAA
GCTAAGGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTT
CATACCTTGGTAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACC
GTCCGCTGGAGCTTTGTGGAATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCA
AGTATGATGAACCAGGGCAACGTGTACGGCCAGATCACCGTACGCATGCACACCCGGCAG
ACTCTGGCCATCTATGACCGGTTTGGCCGGTTGATGTATGGACAGGAAGATGTACCCAAG
GATGTCCTGGAGTATGTTGTATTCGAAAAGCAGTTGACAAACCCCTATGGAAGCTGGAGA
ATGCATACCAAGATCGTTCCCCCATGGGCACCCCCTAAGCAGCCCATCCTTAAGACGGTG
ATGATCCCTGGCCCTCAGCTGAAACCAGAAGAAGAATATGAAGAGGCACAAGGAGAGGCC
CAGAAGCCTCAGCTAGCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MRPL45
Symbol Nomenclature : MRPL45
SYNONYM : L45mt; MRP-L45
Designation : 39S ribosomal protein L45, mitochondrial
Full Nomenclature : mitochondrial ribosomal protein L45
GENEID : 84311
GenBank Accession : HQ258365
HGNC : 16651
MIM : 611850
Vega : OTTHUMG00000133120
Target GenBank: HQ258365

 

Reference Sequence Alignment

  

HsCD00403746      ATGGCAGCCCCCATACCTCAAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTT
NM_032351.4       ATGGCAGCCCCCATACCTCAAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTT
                  ************************************************************

HsCD00403746      CGGCAGCCAGTTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGT
NM_032351.4       CGGCAGCCAGTTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGT
                  ************************************************************

HsCD00403746      TTCACACCTCCTATTTATCAACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCC
NM_032351.4       TTCACACCTCCTATTTATCAACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCC
                  ************************************************************

HsCD00403746      CGGAAAGCAGGATTGGTTATTCCTCCAGAAAAATCGGACCGTTCCATACATCTGGCCTGT
NM_032351.4       CGGAAAGCAGGATTGGTTATTCCTCCAGAAAAATCGGACCGTTCCATACATCTGGCCTGT
                  ************************************************************

HsCD00403746      ACAGCTGGTATATTTGATGCCTATGTTCCTCCTGAGGGTGATGCACGCATATCATCTCTT
NM_032351.4       ACAGCTGGTATATTTGATGCCTATGTTCCTCCTGAGGGTGATGCACGCATATCATCTCTT
                  ************************************************************

HsCD00403746      TCAAAGGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGCATCACAAGTG
NM_032351.4       TCAAAGGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGCATCACAAGTG
                  ************************************************************

HsCD00403746      TCAATCCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAA
NM_032351.4       TCAATCCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAA
                  ************************************************************

HsCD00403746      GCTAAGGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTT
NM_032351.4       GCTAAGGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTT
                  ************************************************************

HsCD00403746      CATACCTTGGTAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACC
NM_032351.4       CATACCTTGGTAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACC
                  ************************************************************

HsCD00403746      GTCCGCTGGAGCTTTGTGGAATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCA
NM_032351.4       GTCCGCTGGAGCTTTGTGGAATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCA
                  ************************************************************

HsCD00403746      AGTATGATGAACCAGGGCAACGTGTACGGCCAGATCACCGTACGCATGCACACCCGGCAG
NM_032351.4       AGTATGATGAACCAGGGCAACGTGTACGGCCAGATCACCGTACGCATGCACACCCGGCAG
                  ************************************************************

HsCD00403746      ACTCTGGCCATCTATGACCGGTTTGGCCGGTTGATGTATGGACAGGAAGATGTACCCAAG
NM_032351.4       ACTCTGGCCATCTATGACCGGTTTGGCCGGTTGATGTATGGACAGGAAGATGTACCCAAG
                  ************************************************************

HsCD00403746      GATGTCCTGGAGTATGTTGTATTCGAAAAGCAGTTGACAAACCCCTATGGAAGCTGGAGA
NM_032351.4       GATGTCCTGGAGTATGTTGTATTCGAAAAGCAGTTGACAAACCCCTATGGAAGCTGGAGA
                  ************************************************************

HsCD00403746      ATGCATACCAAGATCGTTCCCCCATGGGCACCCCCTAAGCAGCCCATCCTTAAGACGGTG
NM_032351.4       ATGCATACCAAGATCGTTCCCCCATGGGCACCCCCTAAGCAGCCCATCCTTAAGACGGTG
                  ************************************************************

HsCD00403746      ATGATCCCTGGCCCTCAGCTGAAACCAGAAGAAGAATATGAAGAGGCACAAGGAGAGGCC
NM_032351.4       ATGATCCCTGGCCCTCAGCTGAAACCAGAAGAAGAATATGAAGAGGCACAAGGAGAGGCC
                  ************************************************************

HsCD00403746      CAGAAGCCTCAGCTAGCC---
NM_032351.4       CAGAAGCCTCAGCTAGCCTGA
                  ******************   

Protein

SMART domain : Tim44 :
HPRD : 14764

Clone

Cloning Information : 574578

Organism


TAX_ID : 9606
Species Specific ID: 84311

Genome

Chromosome : 17
Map Location : 17q21.2
Ensembl : ENSG00000174100

Reagents

Labome : MRPL45-antibody