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RNASE9 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RNASE9
Gene Name:  ribonuclease, RNase A family, 9 (non-active)
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100072777)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00403787

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 615nts         Open reading frame : 1 to 615
Reference Sequence Alignment

ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTATTGCCGCAGCAGCTG
CTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTCCCAGAAGAAGATAAA
AAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGGCCCGCCAGACCACCT
ACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATGCCACTAAATCATATA
GAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAACACCGTTGGGTGGCA
GAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATCTGTTACAACAGATTT
GTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGTCTTGTAGAAGGAGTG
TATTGTAATTTAACAGAAGCATCTGAAATACCAGCGTGTAAATACGAATCACTTTATAGG
AAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAAAAACGTATTCCTCAT
ACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTCAGTGAGGATGGTGTC
TTTGTCATACCGCCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNASE9
Symbol Nomenclature : RNASE9
SYNONYM : HEL128; RAK1; h461
Designation : inactive ribonuclease-like protein 9|ribonuclear enzyme|ribonuclease A K1
Full Nomenclature : ribonuclease, RNase A family, 9 (non-active)
GENEID : 390443
GenBank Accession : HQ258747
HGNC : 20673
MIM : 614014
Vega : OTTHUMG00000170989
Target GenBank: HQ258747

 

Reference Sequence Alignment

  

HsCD00403787        ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110359.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001289110.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110356.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001001673.3      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110361.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110357.1      ---------------ATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110360.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
NM_001110358.1      ATGTCTGCAGGAAAAATGATGAGAACTCTCATCACCACACACCCACTGCCCCTGCTTCTA
                                   *********************************************

HsCD00403787        TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110359.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001289110.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110356.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001001673.3      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110361.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110357.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110360.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
NM_001110358.1      TTGCCGCAGCAGCTGCTGCAGCTGGTGCAGTTTCAAGAGGTGGATACAGATTTTGATTTC
                    ************************************************************

HsCD00403787        CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110359.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001289110.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110356.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001001673.3      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110361.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110357.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110360.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
NM_001110358.1      CCAGAAGAAGATAAAAAAGAAGAATTTGAAGAGTGTTTGGAAAAATTTTTTAGTACAGGG
                    ************************************************************

HsCD00403787        CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110359.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001289110.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110356.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001001673.3      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110361.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110357.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110360.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
NM_001110358.1      CCCGCCAGACCACCTACCAAAGAAAAAGTCAAAAGACGTGTCCTTATTGAACCTGGAATG
                    ************************************************************

HsCD00403787        CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110359.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001289110.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110356.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001001673.3      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110361.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110357.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110360.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
NM_001110358.1      CCACTAAATCATATAGAGTACTGTAACCATGAAATCATGGGAAAAAATGTTTACTACAAA
                    ************************************************************

HsCD00403787        CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110359.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001289110.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110356.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001001673.3      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110361.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110357.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110360.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
NM_001110358.1      CACCGTTGGGTGGCAGAACATTACTTCCTTCTTATGCAATATGACGAGCTCCAAAAAATC
                    ************************************************************

HsCD00403787        TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110359.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001289110.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110356.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001001673.3      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110361.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110357.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110360.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
NM_001110358.1      TGTTACAACAGATTTGTGCCATGTAAGAATGGAATTAGGAAATGTAACAGGAGCAAAGGT
                    ************************************************************

HsCD00403787        CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATCTGAAATACCAGCGTGTAAATAC
NM_001110359.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001289110.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110356.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001001673.3      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110361.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110357.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110360.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
NM_001110358.1      CTTGTAGAAGGAGTGTATTGTAATTTAACAGAAGCATTTGAAATACCAGCGTGTAAATAC
                    ************************************* **********************

HsCD00403787        GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110359.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001289110.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110356.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001001673.3      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110361.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110357.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110360.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
NM_001110358.1      GAATCACTTTATAGGAAGGGCTACGTCCTTATCACTTGTTCATGGCAAAATGAAATGCAA
                    ************************************************************

HsCD00403787        AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110359.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001289110.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110356.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001001673.3      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110361.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110357.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110360.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
NM_001110358.1      AAACGTATTCCTCATACTATAAATGATCTCGTGGAGCCACCTGAACACAGAAGTTTCCTC
                    ************************************************************

HsCD00403787        AGTGAGGATGGTGTCTTTGTCATACCGCCC---
NM_001110359.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001289110.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110356.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001001673.3      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110361.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110357.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110360.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
NM_001110358.1      AGTGAGGATGGTGTCTTTGTCATATCGCCCTAG
                    ************************ *****   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 17983

Clone

Cloning Information : 574626

Organism


TAX_ID : 9606
Species Specific ID: 390443

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000188655

Reagents

Labome : RNASE9-antibody