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CAPN13 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN13
Gene Name:  calpain 13
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100072647)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404074

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2007nts         Open reading frame : 1 to 2007
Reference Sequence Alignment

ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGACC
GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCATGTCAAGATTTC
CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
GAGTGGATGAGCCTGGTCATGTACAAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN13
Symbol Nomenclature : CAPN13
Designation : CANP 13|calcium-activated neutral proteinase 13|calpain-13
Full Nomenclature : calpain 13
GENEID : 92291
GenBank Accession : HQ258338
HGNC : 16663
MIM : 610228
Vega : OTTHUMG00000152053
Target GenBank: HQ258338

 

Reference Sequence Alignment

  

HsCD00404074      ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
NM_144575.2       ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
                  ************************************************************

HsCD00404074      TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
NM_144575.2       TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
                  ************************************************************

HsCD00404074      CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
NM_144575.2       CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
                  ************************************************************

HsCD00404074      ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
NM_144575.2       ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
                  ************************************************************

HsCD00404074      AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
NM_144575.2       AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
                  ************************************************************

HsCD00404074      TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
NM_144575.2       TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
                  ************************************************************

HsCD00404074      CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
NM_144575.2       CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
                  ************************************************************

HsCD00404074      ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
NM_144575.2       ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
                  ************************************************************

HsCD00404074      AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
NM_144575.2       AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
                  ************************************************************

HsCD00404074      TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
NM_144575.2       TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
                  ************************************************************

HsCD00404074      ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
NM_144575.2       ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
                  ************************************************************

HsCD00404074      AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
NM_144575.2       AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
                  ************************************************************

HsCD00404074      ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
NM_144575.2       ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
                  ************************************************************

HsCD00404074      TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGACC
NM_144575.2       TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGGCC
                  *********************************************************.**

HsCD00404074      GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
NM_144575.2       GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
                  ************************************************************

HsCD00404074      AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCATGTCAAGATTTC
NM_144575.2       AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCGTGTCAAGATTTC
                  ***********************************************.************

HsCD00404074      CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
NM_144575.2       CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
                  ************************************************************

HsCD00404074      AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
NM_144575.2       AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
                  ************************************************************

HsCD00404074      ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
NM_144575.2       ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
                  ************************************************************

HsCD00404074      GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
NM_144575.2       GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
                  ************************************************************

HsCD00404074      GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
NM_144575.2       GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
                  ************************************************************

HsCD00404074      AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
NM_144575.2       AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
                  ************************************************************

HsCD00404074      TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
NM_144575.2       TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
                  ************************************************************

HsCD00404074      CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
NM_144575.2       CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
                  ************************************************************

HsCD00404074      GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
NM_144575.2       GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
                  ************************************************************

HsCD00404074      TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
NM_144575.2       TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
                  ************************************************************

HsCD00404074      CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
NM_144575.2       CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
                  ************************************************************

HsCD00404074      GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
NM_144575.2       GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
                  ************************************************************

HsCD00404074      GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
NM_144575.2       GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
                  ************************************************************

HsCD00404074      CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
NM_144575.2       CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
                  ************************************************************

HsCD00404074      TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
NM_144575.2       TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
                  ************************************************************

HsCD00404074      GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
NM_144575.2       GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
                  ************************************************************

HsCD00404074      ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
NM_144575.2       ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
                  ************************************************************

HsCD00404074      GAGTGGATGAGCCTGGTCATGTACAAC---
NM_144575.2       GAGTGGATGAGCCTGGTCATGTACAACTGA
                  ***************************   

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
HPRD : 07008

Clone

Cloning Information : 574928

Organism


TAX_ID : 9606
Species Specific ID: 92291

Genome

Chromosome : 2
Map Location : 2p22-p21
Ensembl : ENSG00000162949

Reagents

Labome : CAPN13-antibody