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C1R (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  C1R
Gene Name:  complement component 1, r subcomponent
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073261)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404462

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2115nts         Open reading frame : 51 to 2165
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGTGGCTCT
TGTACCTCCTGGTGCCGGCCCTGTTCTGCAGGGCAGGAGGCTCCATTCCCATCCCTCAGA
AGTTATTTGGGGAGGTGACTTCCCCTCTGTTCCCCAAGCCTTACCCCAACAACTTTGAAA
CAACCACTGTGATCACAGTCCCCACGGGATACAGGGTGAAGCTCGTCTTCCAGCAGTTTG
ACCTGGAGCCTTCTGAAGGCTGCTTCTATGATTATGTCAAGATCTCTGCTGATAAGAAAA
GCCTGGGGAGGTTCTGTGGGCAACTGGGTTCTCCACTGGGCAACCCCCCGGGAAAGAAGG
AATTTATGTCCCAAGGGAACAAGATGCTGCTGACCTTCCACACAGACTTCTCCAACGAGG
AGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAAGCTGTGGACCTTG
ATGAATGTGCTTCCCGGAGCAAATTAGGGGAGGAGGATCCCCAGCCCCAGTGCCAGCACC
TGTGTCACAACTACGTTGGAGGCTACTTCTGTTCCTGCCGTCCAGGCTATGAGCTTCAGG
AAGACAGGCATTCCTGCCAGGCTGAGTGCAGCAGCGAGCTGTACACGGAGGCATCAGGCT
ACATCTCCAGCCTGGAGTACCCTCGGTCCTACCCCCCTGACCTGCGCTGCAACTACAGCA
TCCGGGTGGAGCGGGGCCTCACCCTGCACCTCAAGTTCCTGGAGCCTTTTGATATTGATG
ACCACCAGCAAGTACACTGCCCCTATGACCAGCTACAGATCTATGCCAACGGGAAGAACA
TTGGCGAGTTCTGTGGGAAGCAAAGGCCCCCCGACCTCGACACCAGCAGCAATGCTGTGG
ATCTGCTGTTCTTCACAGATGAGTCGGGGGACAGCCGGGGCTGGAAGCTGCGCTACACCA
CCGAGATCATCAAGTGCCCCCAGCCCAAGACCCTAGACGAGTTCACCATCATCCAGAACC
TGCAGCCTCAGTACCAGTTCCGTGACTACTTCATTGCTACCTGCAAGCAAGGCTACCAGC
TCATAGAGGGGAACCAGGTGCTGCATTCCTTCACAGCTGTCTGCCAGGATGATGGCACGT
GGCATCGTGCCATGCCCAGATGCAAGATCAAGGACTGTGGGCAGCCCCGAAACCTGCCTA
ATGGTGACTTCCGTTACACCACCACAATGGGAGTGAACACCTACAAGGCCCGTATCCAGT
ACTACTGCCATGAGCCATATTACAAGATGCAGACCAGAGCTGGCAGCAGGGAGTCTGAGC
AAGGGGTGTACACCTGCACAGCACAGGGCATTTGGAAGAATGAACAGAAGGGAGAGAAGA
TTCCTCGGTGCTTGCCAGTGTGTGGGAAGCCCGTGAACCCCGTGGAACAGAGGCAGCGCA
TCATCGGAGGGCAAAAAGCCAAGATGGGCAACTTCCCCTGGCAGGTGTTCACCAACATCC
ACGGGCGCGGGGGCGGGGCCCTGCTGGGCGACCGCTGGATCCTCACAGCTGCCCACACCC
TGTATCCCAAGGAACACGAAGCGCAAAGCAACGCCTCTTTGGATGTGTTCCTGGGCCACA
CAAATGTGGAAGAGCTCATGAAGCTAGGAAATCACCCCATCCGCAGGGTCAGCGTCCACC
CGGACTACCGTCAGGATGAGTCCTACAATTTTGAGGGGGACATCGCCCTGCTGGAGCTGG
AAAATAGTGTCACCCTGGGTCCCAACCTCCTCCCCATCTGCCTCCCTGACAACGATACCT
TCTACGACCTGGGCTTGATGGGCTATGTCAGTGGCTTCGGGGTCATGGAGGAGAAGATTG
CTCATGACCTCAGGTTTGTCCGTCTGCCCGTAGCTAATCCACAGGCCTGTGAGAACTGGC
TCCGGGGAAAGAATAGGATGGATGTGTTCTCTCAAAACATGTTCTGTGCTGGACACCCAT
CTCTAAAGCAGGACGCCTGCCAGGGGGATAGTGGGGGCGTTTTTGCAGTAAGGGACCCGA
ACACTGATCGCTGGGTGGCCACGGGCATCGTGTCCTGGGGCATCGGGTGCAGCAGGGGCT
ATGGCTTCTACACCAAAGTGCTCAACTACGTGGACTGGATCAAGAAAGAGATGGAGGAGG
AGGACTGCCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : C1R
Symbol Nomenclature : C1R
Designation : complement C1r subcomponent
Full Nomenclature : complement component 1, r subcomponent
GENEID : 715
GenBank Accession : JF432122
HGNC : 1246
MIM : 613785
Target GenBank: JF432122

 

Reference Sequence Alignment

  

HsCD00404462      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGTGGCTCT
NM_001733.4       --------------------------------------------------ATGTGGCTCT
                                                                    **********

HsCD00404462      TGTACCTCCTGGTGCCGGCCCTGTTCTGCAGGGCAGGAGGCTCCATTCCCATCCCTCAGA
NM_001733.4       TGTACCTCCTGGTGCCGGCCCTGTTCTGCAGGGCAGGAGGCTCCATTCCCATCCCTCAGA
                  ************************************************************

HsCD00404462      AGTTATTTGGGGAGGTGACTTCCCCTCTGTTCCCCAAGCCTTACCCCAACAACTTTGAAA
NM_001733.4       AGTTATTTGGGGAGGTGACTTCCCCTCTGTTCCCCAAGCCTTACCCCAACAACTTTGAAA
                  ************************************************************

HsCD00404462      CAACCACTGTGATCACAGTCCCCACGGGATACAGGGTGAAGCTCGTCTTCCAGCAGTTTG
NM_001733.4       CAACCACTGTGATCACAGTCCCCACGGGATACAGGGTGAAGCTCGTCTTCCAGCAGTTTG
                  ************************************************************

HsCD00404462      ACCTGGAGCCTTCTGAAGGCTGCTTCTATGATTATGTCAAGATCTCTGCTGATAAGAAAA
NM_001733.4       ACCTGGAGCCTTCTGAAGGCTGCTTCTATGATTATGTCAAGATCTCTGCTGATAAGAAAA
                  ************************************************************

HsCD00404462      GCCTGGGGAGGTTCTGTGGGCAACTGGGTTCTCCACTGGGCAACCCCCCGGGAAAGAAGG
NM_001733.4       GCCTGGGGAGGTTCTGTGGGCAACTGGGTTCTCCACTGGGCAACCCCCCGGGAAAGAAGG
                  ************************************************************

HsCD00404462      AATTTATGTCCCAAGGGAACAAGATGCTGCTGACCTTCCACACAGACTTCTCCAACGAGG
NM_001733.4       AATTTATGTCCCAAGGGAACAAGATGCTGCTGACCTTCCACACAGACTTCTCCAACGAGG
                  ************************************************************

HsCD00404462      AGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAAGCTGTGGACCTTG
NM_001733.4       AGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAAGCTGTGGACCTTG
                  ************************************************************

HsCD00404462      ATGAATGTGCTTCCCGGAGCAAATTAGGGGAGGAGGATCCCCAGCCCCAGTGCCAGCACC
NM_001733.4       ATGAATGTGCTTCCCGGAGCAAATTAGGGGAGGAGGATCCCCAGCCCCAGTGCCAGCACC
                  ************************************************************

HsCD00404462      TGTGTCACAACTACGTTGGAGGCTACTTCTGTTCCTGCCGTCCAGGCTATGAGCTTCAGG
NM_001733.4       TGTGTCACAACTACGTTGGAGGCTACTTCTGTTCCTGCCGTCCAGGCTATGAGCTTCAGG
                  ************************************************************

HsCD00404462      AAGACAGGCATTCCTGCCAGGCTGAGTGCAGCAGCGAGCTGTACACGGAGGCATCAGGCT
NM_001733.4       AAGACAGGCATTCCTGCCAGGCTGAGTGCAGCAGCGAGCTGTACACGGAGGCATCAGGCT
                  ************************************************************

HsCD00404462      ACATCTCCAGCCTGGAGTACCCTCGGTCCTACCCCCCTGACCTGCGCTGCAACTACAGCA
NM_001733.4       ACATCTCCAGCCTGGAGTACCCTCGGTCCTACCCCCCTGACCTGCGCTGCAACTACAGCA
                  ************************************************************

HsCD00404462      TCCGGGTGGAGCGGGGCCTCACCCTGCACCTCAAGTTCCTGGAGCCTTTTGATATTGATG
NM_001733.4       TCCGGGTGGAGCGGGGCCTCACCCTGCACCTCAAGTTCCTGGAGCCTTTTGATATTGATG
                  ************************************************************

HsCD00404462      ACCACCAGCAAGTACACTGCCCCTATGACCAGCTACAGATCTATGCCAACGGGAAGAACA
NM_001733.4       ACCACCAGCAAGTACACTGCCCCTATGACCAGCTACAGATCTATGCCAACGGGAAGAACA
                  ************************************************************

HsCD00404462      TTGGCGAGTTCTGTGGGAAGCAAAGGCCCCCCGACCTCGACACCAGCAGCAATGCTGTGG
NM_001733.4       TTGGCGAGTTCTGTGGGAAGCAAAGGCCCCCCGACCTCGACACCAGCAGCAATGCTGTGG
                  ************************************************************

HsCD00404462      ATCTGCTGTTCTTCACAGATGAGTCGGGGGACAGCCGGGGCTGGAAGCTGCGCTACACCA
NM_001733.4       ATCTGCTGTTCTTCACAGATGAGTCGGGGGACAGCCGGGGCTGGAAGCTGCGCTACACCA
                  ************************************************************

HsCD00404462      CCGAGATCATCAAGTGCCCCCAGCCCAAGACCCTAGACGAGTTCACCATCATCCAGAACC
NM_001733.4       CCGAGATCATCAAGTGCCCCCAGCCCAAGACCCTAGACGAGTTCACCATCATCCAGAACC
                  ************************************************************

HsCD00404462      TGCAGCCTCAGTACCAGTTCCGTGACTACTTCATTGCTACCTGCAAGCAAGGCTACCAGC
NM_001733.4       TGCAGCCTCAGTACCAGTTCCGTGACTACTTCATTGCTACCTGCAAGCAAGGCTACCAGC
                  ************************************************************

HsCD00404462      TCATAGAGGGGAACCAGGTGCTGCATTCCTTCACAGCTGTCTGCCAGGATGATGGCACGT
NM_001733.4       TCATAGAGGGGAACCAGGTGCTGCATTCCTTCACAGCTGTCTGCCAGGATGATGGCACGT
                  ************************************************************

HsCD00404462      GGCATCGTGCCATGCCCAGATGCAAGATCAAGGACTGTGGGCAGCCCCGAAACCTGCCTA
NM_001733.4       GGCATCGTGCCATGCCCAGATGCAAGATCAAGGACTGTGGGCAGCCCCGAAACCTGCCTA
                  ************************************************************

HsCD00404462      ATGGTGACTTCCGTTACACCACCACAATGGGAGTGAACACCTACAAGGCCCGTATCCAGT
NM_001733.4       ATGGTGACTTCCGTTACACCACCACAATGGGAGTGAACACCTACAAGGCCCGTATCCAGT
                  ************************************************************

HsCD00404462      ACTACTGCCATGAGCCATATTACAAGATGCAGACCAGAGCTGGCAGCAGGGAGTCTGAGC
NM_001733.4       ACTACTGCCATGAGCCATATTACAAGATGCAGACCAGAGCTGGCAGCAGGGAGTCTGAGC
                  ************************************************************

HsCD00404462      AAGGGGTGTACACCTGCACAGCACAGGGCATTTGGAAGAATGAACAGAAGGGAGAGAAGA
NM_001733.4       AAGGGGTGTACACCTGCACAGCACAGGGCATTTGGAAGAATGAACAGAAGGGAGAGAAGA
                  ************************************************************

HsCD00404462      TTCCTCGGTGCTTGCCAGTGTGTGGGAAGCCCGTGAACCCCGTGGAACAGAGGCAGCGCA
NM_001733.4       TTCCTCGGTGCTTGCCAGTGTGTGGGAAGCCCGTGAACCCCGTGGAACAGAGGCAGCGCA
                  ************************************************************

HsCD00404462      TCATCGGAGGGCAAAAAGCCAAGATGGGCAACTTCCCCTGGCAGGTGTTCACCAACATCC
NM_001733.4       TCATCGGAGGGCAAAAAGCCAAGATGGGCAACTTCCCCTGGCAGGTGTTCACCAACATCC
                  ************************************************************

HsCD00404462      ACGGGCGCGGGGGCGGGGCCCTGCTGGGCGACCGCTGGATCCTCACAGCTGCCCACACCC
NM_001733.4       ACGGGCGCGGGGGCGGGGCCCTGCTGGGCGACCGCTGGATCCTCACAGCTGCCCACACCC
                  ************************************************************

HsCD00404462      TGTATCCCAAGGAACACGAAGCGCAAAGCAACGCCTCTTTGGATGTGTTCCTGGGCCACA
NM_001733.4       TGTATCCCAAGGAACACGAAGCGCAAAGCAACGCCTCTTTGGATGTGTTCCTGGGCCACA
                  ************************************************************

HsCD00404462      CAAATGTGGAAGAGCTCATGAAGCTAGGAAATCACCCCATCCGCAGGGTCAGCGTCCACC
NM_001733.4       CAAATGTGGAAGAGCTCATGAAGCTAGGAAATCACCCCATCCGCAGGGTCAGCGTCCACC
                  ************************************************************

HsCD00404462      CGGACTACCGTCAGGATGAGTCCTACAATTTTGAGGGGGACATCGCCCTGCTGGAGCTGG
NM_001733.4       CGGACTACCGTCAGGATGAGTCCTACAATTTTGAGGGGGACATCGCCCTGCTGGAGCTGG
                  ************************************************************

HsCD00404462      AAAATAGTGTCACCCTGGGTCCCAACCTCCTCCCCATCTGCCTCCCTGACAACGATACCT
NM_001733.4       AAAATAGTGTCACCCTGGGTCCCAACCTCCTCCCCATCTGCCTCCCTGACAACGATACCT
                  ************************************************************

HsCD00404462      TCTACGACCTGGGCTTGATGGGCTATGTCAGTGGCTTCGGGGTCATGGAGGAGAAGATTG
NM_001733.4       TCTACGACCTGGGCTTGATGGGCTATGTCAGTGGCTTCGGGGTCATGGAGGAGAAGATTG
                  ************************************************************

HsCD00404462      CTCATGACCTCAGGTTTGTCCGTCTGCCCGTAGCTAATCCACAGGCCTGTGAGAACTGGC
NM_001733.4       CTCATGACCTCAGGTTTGTCCGTCTGCCCGTAGCTAATCCACAGGCCTGTGAGAACTGGC
                  ************************************************************

HsCD00404462      TCCGGGGAAAGAATAGGATGGATGTGTTCTCTCAAAACATGTTCTGTGCTGGACACCCAT
NM_001733.4       TCCGGGGAAAGAATAGGATGGATGTGTTCTCTCAAAACATGTTCTGTGCTGGACACCCAT
                  ************************************************************

HsCD00404462      CTCTAAAGCAGGACGCCTGCCAGGGGGATAGTGGGGGCGTTTTTGCAGTAAGGGACCCGA
NM_001733.4       CTCTAAAGCAGGACGCCTGCCAGGGGGATAGTGGGGGCGTTTTTGCAGTAAGGGACCCGA
                  ************************************************************

HsCD00404462      ACACTGATCGCTGGGTGGCCACGGGCATCGTGTCCTGGGGCATCGGGTGCAGCAGGGGCT
NM_001733.4       ACACTGATCGCTGGGTGGCCACGGGCATCGTGTCCTGGGGCATCGGGTGCAGCAGGGGCT
                  ************************************************************

HsCD00404462      ATGGCTTCTACACCAAAGTGCTCAACTACGTGGACTGGATCAAGAAAGAGATGGAGGAGG
NM_001733.4       ATGGCTTCTACACCAAAGTGCTCAACTACGTGGACTGGATCAAGAAAGAGATGGAGGAGG
                  ************************************************************

HsCD00404462      AGGACTGCCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_001733.4       AGGACTGA-------------------------------------------------
                  *******.                                                 

Pathways

Reactome : Classical antibody-mediated complement activation
Reactome : Complement cascade
Reactome : Creation of C4 and C2 activators
Reactome : Immune System
Reactome : Initial triggering of complement
Reactome : Innate Immune System

Clone

Cloning Information : 579429

Organism


TAX_ID : 9606
Species Specific ID: 715

Genome

Chromosome : 12
Map Location : 12p13

Reagents

Labome : C1R-antibody