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LRRC45 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LRRC45
Gene Name:  leucine rich repeat containing 45
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073269)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404508

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2010nts         Open reading frame : 51 to 2060
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGAGGAGT
TCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAGGAGGCTGTCC
TGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAAAGCCTGACGG
TGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGCACGGAGCTGG
TCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGAGGCCTGTGTG
CCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCTGCAGGGGCCG
AGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACGCTGGAGTGGA
ACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTGGCGGCCAACG
GCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGCGCGGAGGAGC
TGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGCTGGAATAACG
TTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGAACCCTGTGGA
GACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTGGAGCAAGCCA
TGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGCACTCACGTCC
TCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTCGACTTGATGG
AGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCGGCAGCCCGTG
TAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCTCTCAAGGCCA
AGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAGGACCTGGGGG
AGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAGGCCAAGGAGC
TCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGAGACTTGTCTG
CTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAGCGGAAGTTCA
GGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATGTCAGCTGAGC
TGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGAAGATGCAGAC
AGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATGACCCGTCACC
TGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCGGCGCGGCTGT
CCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGTGGCCAGGCTG
AGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAGCGCCTGGCTC
ACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAGGGCGCTTGCC
TACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTGGGCCTGCAAG
TTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTCAAGGACCAGG
AAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAACGGCCGGCTGC
AGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTGGAGCGCCAGC
TGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGCGAGAACGCGT
CTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGGGACGAGGAGG
CCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCGTCCCCCGTGA
GGACCCTGAGCCCCCCAAAGTGCCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGC
ATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LRRC45
Symbol Nomenclature : LRRC45
Designation : leucine-rich repeat-containing protein 45
Full Nomenclature : leucine rich repeat containing 45
GENEID : 201255
GenBank Accession : JF432130
HGNC : 28302
Vega : OTTHUMG00000178454
Target GenBank: JF432130

 

Reference Sequence Alignment

  

HsCD00404508      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGAGGAGT
NM_144999.2       --------------------------------------------------ATGGAGGAGT
                                                                    **********

HsCD00404508      TCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAGGAGGCTGTCC
NM_144999.2       TCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAGGAGGCTGTCC
                  ************************************************************

HsCD00404508      TGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAAAGCCTGACGG
NM_144999.2       TGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAAAGCCTGACGG
                  ************************************************************

HsCD00404508      TGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGCACGGAGCTGG
NM_144999.2       TGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGCACGGAGCTGG
                  ************************************************************

HsCD00404508      TCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGAGGCCTGTGTG
NM_144999.2       TCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGAGGCCTGTGTG
                  ************************************************************

HsCD00404508      CCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCTGCAGGGGCCG
NM_144999.2       CCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCTGCAGGGGCCG
                  ************************************************************

HsCD00404508      AGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACGCTGGAGTGGA
NM_144999.2       AGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACGCTGGAGTGGA
                  ************************************************************

HsCD00404508      ACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTGGCGGCCAACG
NM_144999.2       ACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTGGCGGCCAACG
                  ************************************************************

HsCD00404508      GCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGCGCGGAGGAGC
NM_144999.2       GCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGCGCGGAGGAGC
                  ************************************************************

HsCD00404508      TGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGCTGGAATAACG
NM_144999.2       TGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGCTGGAATAACG
                  ************************************************************

HsCD00404508      TTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGAACCCTGTGGA
NM_144999.2       TTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGAACCCTGTGGA
                  ************************************************************

HsCD00404508      GACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTGGAGCAAGCCA
NM_144999.2       GACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTGGAGCAAGCCA
                  ************************************************************

HsCD00404508      TGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGCACTCACGTCC
NM_144999.2       TGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGCACTCACGTCC
                  ************************************************************

HsCD00404508      TCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTCGACTTGATGG
NM_144999.2       TCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTCGACTTGATGG
                  ************************************************************

HsCD00404508      AGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCGGCAGCCCGTG
NM_144999.2       AGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCGGCAGCCCGTG
                  ************************************************************

HsCD00404508      TAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCTCTCAAGGCCA
NM_144999.2       TAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCTCTCAAGGCCA
                  ************************************************************

HsCD00404508      AGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAGGACCTGGGGG
NM_144999.2       AGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAGGACCTGGGGG
                  ************************************************************

HsCD00404508      AGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAGGCCAAGGAGC
NM_144999.2       AGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAGGCCAAGGAGC
                  ************************************************************

HsCD00404508      TCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGAGACTTGTCTG
NM_144999.2       TCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGAGACTTGTCTG
                  ************************************************************

HsCD00404508      CTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAGCGGAAGTTCA
NM_144999.2       CTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAGCGGAAGTTCA
                  ************************************************************

HsCD00404508      GGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATGTCAGCTGAGC
NM_144999.2       GGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATGTCAGCTGAGC
                  ************************************************************

HsCD00404508      TGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGAAGATGCAGAC
NM_144999.2       TGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGAAGATGCAGAC
                  ************************************************************

HsCD00404508      AGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATGACCCGTCACC
NM_144999.2       AGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATGACCCGTCACC
                  ************************************************************

HsCD00404508      TGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCGGCGCGGCTGT
NM_144999.2       TGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCGGCGCGGCTGT
                  ************************************************************

HsCD00404508      CCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGTGGCCAGGCTG
NM_144999.2       CCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGTGGCCAGGCTG
                  ************************************************************

HsCD00404508      AGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAGCGCCTGGCTC
NM_144999.2       AGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAGCGCCTGGCTC
                  ************************************************************

HsCD00404508      ACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAGGGCGCTTGCC
NM_144999.2       ACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAGGGCGCTTGCC
                  ************************************************************

HsCD00404508      TACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTGGGCCTGCAAG
NM_144999.2       TACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTGGGCCTGCAAG
                  ************************************************************

HsCD00404508      TTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTCAAGGACCAGG
NM_144999.2       TTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTCAAGGACCAGG
                  ************************************************************

HsCD00404508      AAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAACGGCCGGCTGC
NM_144999.2       AAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAACGGCCGGCTGC
                  ************************************************************

HsCD00404508      AGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTGGAGCGCCAGC
NM_144999.2       AGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTGGAGCGCCAGC
                  ************************************************************

HsCD00404508      TGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGCGAGAACGCGT
NM_144999.2       TGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGCGAGAACGCGT
                  ************************************************************

HsCD00404508      CTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGGGACGAGGAGG
NM_144999.2       CTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGGGACGAGGAGG
                  ************************************************************

HsCD00404508      CCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCGTCCCCCGTGA
NM_144999.2       CCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCGTCCCCCGTGA
                  ************************************************************

HsCD00404508      GGACCCTGAGCCCCCCAAAGTGCCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGC
NM_144999.2       GGACCCTGAGCCCCCCAAAGTGA-------------------------------------
                  **********************.                                     

HsCD00404508      ATTGCTTATCAA
NM_144999.2       ------------
                              

Clone

Cloning Information : 579493

Organism


TAX_ID : 9606
Species Specific ID: 201255

Genome

Chromosome : 17
Map Location : 17q25.3
Ensembl : ENSG00000169683

Reagents

Labome : LRRC45-antibody