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CA10 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CA10
Gene Name:  carbonic anhydrase X
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073896)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404696

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 984nts         Open reading frame : 51 to 1034
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGAAATAG
TCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCAGCTCAACAGA
ATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAGGGAAGCTTTG
TTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGCTCTGTGGGGA
AACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCCTTTCTGACAC
CTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAACACTGGAAGAC
ACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGGCCCATGACAT
ACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGCCAAGGGTCGG
AGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCACTATAACCATG
AGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTGGTAGTTTCTA
TATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTCAACAGAGATA
CTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTTAATATAGAGG
AACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACTATCCCACCCT
GCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACCAGGATGCAGA
TGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTGAGCATGAGTG
ACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAATATCAACTTCA
GTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTATAGAGTAAATG
AATGGCTCCTCAAGTACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCT
TATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CA10
Symbol Nomenclature : CA10
SYNONYM : CA-RPX; CARPX; HUCEP-15
Designation : CA-RP X|CARP X|carbonic anhydrase-related protein 10|carbonic anhydrase-related protein X|cerebral protein 15|cerebral protein-15
Full Nomenclature : carbonic anhydrase X
GENEID : 56934
Locus Tag : hucep-15
GenBank Accession : JF432658
HGNC : 1369
MIM : 604642
Vega : OTTHUMG00000177544
Target GenBank: JF432658

 

Reference Sequence Alignment

  

HsCD00404696        TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGAAATAG
NM_020178.4         --------------------------------------------------ATGGAAATAG
NM_001082534.1      --------------------------------------------------ATGGAAATAG
NM_001082533.1      --------------------------------------------------ATGGAAATAG
                                                                      **********

HsCD00404696        TCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCAGCTCAACAGA
NM_020178.4         TCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCAGCTCAACAGA
NM_001082534.1      TCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCAGCTCAACAGA
NM_001082533.1      TCTGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCAGCTCAACAGA
                    ************************************************************

HsCD00404696        ATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAGGGAAGCTTTG
NM_020178.4         ATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAGGGAAGCTTTG
NM_001082534.1      ATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAGGGAAGCTTTG
NM_001082533.1      ATTCACCAAAAATCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAGGGAAGCTTTG
                    ************************************************************

HsCD00404696        TTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGCTCTGTGGGGA
NM_020178.4         TTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGCTCTGTGGGGA
NM_001082534.1      TTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGCTCTGTGGGGA
NM_001082533.1      TTCCAGTTCCTTCTTTCTGGGGATTGGTGAACTCAGCTTGGAATCTTTGCTCTGTGGGGA
                    ************************************************************

HsCD00404696        AACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCCTTTCTGACAC
NM_020178.4         AACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCCTTTCTGACAC
NM_001082534.1      AACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCCTTTCTGACAC
NM_001082533.1      AACGGCAGTCGCCAGTCAACATAGAGACCAGTCACATGATCTTCGACCCCTTTCTGACAC
                    ************************************************************

HsCD00404696        CTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAACACTGGAAGAC
NM_020178.4         CTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAACACTGGAAGAC
NM_001082534.1      CTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAACACTGGAAGAC
NM_001082533.1      CTCTTCGCATCAACACGGGGGGCAGGAAGGTCAGTGGGACCATGTACAACACTGGAAGAC
                    ************************************************************

HsCD00404696        ACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGGCCCATGACAT
NM_020178.4         ACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGGCCCATGACAT
NM_001082534.1      ACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGGCCCATGACAT
NM_001082533.1      ACGTATCCCTTCGCCTGGACAAGGAGCACTTGGTCAACATATCTGGAGGGCCCATGACAT
                    ************************************************************

HsCD00404696        ACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGCCAAGGGTCGG
NM_020178.4         ACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGCCAAGGGTCGG
NM_001082534.1      ACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGCCAAGGGTCGG
NM_001082533.1      ACAGCCACCGGCTGGAGGAGATCCGACTACACTTTGGGAGTGAGGACAGCCAAGGGTCGG
                    ************************************************************

HsCD00404696        AGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCACTATAACCATG
NM_020178.4         AGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCACTATAACCATG
NM_001082534.1      AGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCACTATAACCATG
NM_001082533.1      AGCACCTCCTCAATGGACAGGCCTTCTCTGGGGAGGTGCAGCTCATCCACTATAACCATG
                    ************************************************************

HsCD00404696        AGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTGGTAGTTTCTA
NM_020178.4         AGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTGGTAGTTTCTA
NM_001082534.1      AGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTGGTAGTTTCTA
NM_001082533.1      AGCTATATACGAATGTCACAGAAGCTGCAAAGAGTCCAAATGGATTGGTGGTAGTTTCTA
                    ************************************************************

HsCD00404696        TATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTCAACAGAGATA
NM_020178.4         TATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTCAACAGAGATA
NM_001082534.1      TATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTCAACAGAGATA
NM_001082533.1      TATTTATAAAAGTTTCTGATTCATCAAACCCATTTCTTAATCGAATGCTCAACAGAGATA
                    ************************************************************

HsCD00404696        CTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTTAATATAGAGG
NM_020178.4         CTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTTAATATAGAGG
NM_001082534.1      CTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTTAATATAGAGG
NM_001082533.1      CTATCACAAGAATAACATATAAAAATGATGCATATTTACTACAGGGGCTTAATATAGAGG
                    ************************************************************

HsCD00404696        AACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACTATCCCACCCT
NM_020178.4         AACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACTATCCCACCCT
NM_001082534.1      AACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACTATCCCACCCT
NM_001082533.1      AACTATATCCAGAGACCTCTAGTTTCATCACTTACGATGGGTCGATGACTATCCCACCCT
                    ************************************************************

HsCD00404696        GCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACCAGGATGCAGA
NM_020178.4         GCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACCAGGATGCAGA
NM_001082534.1      GCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACCAGGATGCAGA
NM_001082533.1      GCTATGAGACAGCAAGTTGGATCATAATGAACAAACCTGTCTATATAACCAGGATGCAGA
                    ************************************************************

HsCD00404696        TGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTGAGCATGAGTG
NM_020178.4         TGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTGAGCATGAGTG
NM_001082534.1      TGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTGAGCATGAGTG
NM_001082533.1      TGCATTCCTTGCGCCTGCTCAGCCAGAACCAGCCATCTCAGATCTTTCTGAGCATGAGTG
                    ************************************************************

HsCD00404696        ACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAATATCAACTTCA
NM_020178.4         ACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAATATCAACTTCA
NM_001082534.1      ACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAATATCAACTTCA
NM_001082533.1      ACAACTTCAGGCCTGTCCAGCCACTCAACAACCGCTGCATCCGCACCAATATCAACTTCA
                    ************************************************************

HsCD00404696        GTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTATAGAGTAAATG
NM_020178.4         GTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTATAGAGTAAATG
NM_001082534.1      GTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTATAGAGTAAATG
NM_001082533.1      GTTTACAGGGGAAGGACTGTCCAAACAACCGAGCCCAGAAGCTTCAGTATAGAGTAAATG
                    ************************************************************

HsCD00404696        AATGGCTCCTCAAGTACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCT
NM_020178.4         AATGGCTCCTCAAGTAG-------------------------------------------
NM_001082534.1      AATGGCTCCTCAAGTAG-------------------------------------------
NM_001082533.1      AATGGCTCCTCAAGTAG-------------------------------------------
                    ****************                                            

HsCD00404696        TATCAA
NM_020178.4         ------
NM_001082534.1      ------
NM_001082533.1      ------
                          

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
HPRD : 05224

Clone

Cloning Information : 579714

Organism


TAX_ID : 9606
Species Specific ID: 56934

Genome

Chromosome : 17
Map Location : 17q21.33
Ensembl : ENSG00000154975

Reagents

Labome : CA10-antibody