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CKB (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CKB
Gene Name:  creatine kinase, brain
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073761)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404793

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1143nts         Open reading frame : 51 to 1193
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGCCCTTCT
CCAACAGCCACAACGCACTGAAGCTGCGCTTCCCGGCCGAGGACGAGTTCCCCGACCTGA
GCGCCCACAACAACCACATGGCCAAGGTGCTGACCCCCGAGCTGTACGCGGAGCTGCGCG
CCAAGAGCACGCCGAGCGGCTTCACGCTGGACGACGTCATCCAGACAGGCGTGGACAACC
CGGGCCACCCGTACATCATGACCGTGGGCTGCGTGGCGGGCGACGAGGAGTCCTACGAAG
TGTTCAAGGATCTCTTCGACCCCATCATCGAGGACCGGCACGGCGGCTACAAGCCCAGCG
ATGAGCACAAGACCGACCTCAACCCCGACAACCTGCAGGGCGGCGACGACCTGGACCCCA
ACTACGTGCTGAGCTCGCGGGTGCGCACGGGCCGCAGCATCCGTGGCTTCTGCCTCCCCC
CGCACTGCAGCCGCGGGGAGCGCCGAGCCATCGAGAAGCTCGCGGTGGAAGCCCTGTCCA
GCCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCATGACGGAGGCGGAGC
AGCAGCAGCTCATCGACGACCACTTCCTCTTCGACAAGCCCGTGTCGCCCCTGCTGCTGG
CCTCGGGCATGGCCCGCGACTGGCCCGACGCCCGCGGTATCTGGCACAATGACAATAAGA
CCTTCCTGGTGTGGGTCAACGAGGAGGACCACCTGCGGGTCATCTCCATGCAGAAGGGGG
GCAACATGAAGGAGGTGTTCACCCGCTTCTGCACCGGCCTCACCCAGATTGAAACTCTCT
TCAAGTCTAAGGACTATGAGTTCATGTGGAACCCTCACCTGGGCTACATCCTCACCTGCC
CATCCAACCTGGGCACCGGGCTGCGGGCAGGTGTGCATATCAAGCTGCCCAACCTGGGCA
AGCATGAGAAGTTCTCGGAGGTGCTTAAGCGGCTGCGACTTCAGAAGCGAGGCACAGGCG
GTGTGGACACGGCTGCGGTGGGCGGGGTCTTCGACGTCTCCAACGCTGACCGCCTGGGCT
TCTCAGAGGTGGAGCTGGTGCAGATGGTGGTGGACGGAGTGAAGCTGCTCATCGAGATGG
AACAGCGGCTGGAGCAGGGCCAGGCCATCGACGACCTCATGCCTGCCCAGAAATGCCCAA
CTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CKB
Symbol Nomenclature : CKB
SYNONYM : B-CK; BCK; CKBB; HEL-S-29
Designation : creatine kinase B chain|creatine kinase B-type|creatine kinase-B|epididymis secretory protein Li 29
Full Nomenclature : creatine kinase, brain
GENEID : 1152
GenBank Accession : JF432538
HGNC : 1991
MIM : 123280
Vega : OTTHUMG00000171786
Target GenBank: JF432538

 

Reference Sequence Alignment

  

HsCD00404793      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGCCCTTCT
NM_001823.4       --------------------------------------------------ATGCCCTTCT
                                                                    **********

HsCD00404793      CCAACAGCCACAACGCACTGAAGCTGCGCTTCCCGGCCGAGGACGAGTTCCCCGACCTGA
NM_001823.4       CCAACAGCCACAACGCACTGAAGCTGCGCTTCCCGGCCGAGGACGAGTTCCCCGACCTGA
                  ************************************************************

HsCD00404793      GCGCCCACAACAACCACATGGCCAAGGTGCTGACCCCCGAGCTGTACGCGGAGCTGCGCG
NM_001823.4       GCGCCCACAACAACCACATGGCCAAGGTGCTGACCCCCGAGCTGTACGCGGAGCTGCGCG
                  ************************************************************

HsCD00404793      CCAAGAGCACGCCGAGCGGCTTCACGCTGGACGACGTCATCCAGACAGGCGTGGACAACC
NM_001823.4       CCAAGAGCACGCCGAGCGGCTTCACGCTGGACGACGTCATCCAGACAGGCGTGGACAACC
                  ************************************************************

HsCD00404793      CGGGCCACCCGTACATCATGACCGTGGGCTGCGTGGCGGGCGACGAGGAGTCCTACGAAG
NM_001823.4       CGGGCCACCCGTACATCATGACCGTGGGCTGCGTGGCGGGCGACGAGGAGTCCTACGAAG
                  ************************************************************

HsCD00404793      TGTTCAAGGATCTCTTCGACCCCATCATCGAGGACCGGCACGGCGGCTACAAGCCCAGCG
NM_001823.4       TGTTCAAGGATCTCTTCGACCCCATCATCGAGGACCGGCACGGCGGCTACAAGCCCAGCG
                  ************************************************************

HsCD00404793      ATGAGCACAAGACCGACCTCAACCCCGACAACCTGCAGGGCGGCGACGACCTGGACCCCA
NM_001823.4       ATGAGCACAAGACCGACCTCAACCCCGACAACCTGCAGGGCGGCGACGACCTGGACCCCA
                  ************************************************************

HsCD00404793      ACTACGTGCTGAGCTCGCGGGTGCGCACGGGCCGCAGCATCCGTGGCTTCTGCCTCCCCC
NM_001823.4       ACTACGTGCTGAGCTCGCGGGTGCGCACGGGCCGCAGCATCCGTGGCTTCTGCCTCCCCC
                  ************************************************************

HsCD00404793      CGCACTGCAGCCGCGGGGAGCGCCGAGCCATCGAGAAGCTCGCGGTGGAAGCCCTGTCCA
NM_001823.4       CGCACTGCAGCCGCGGGGAGCGCCGCGCCATCGAGAAGCTCGCGGTGGAAGCCCTGTCCA
                  *************************.**********************************

HsCD00404793      GCCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCATGACGGAGGCGGAGC
NM_001823.4       GCCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCATGACGGAGGCGGAGC
                  ************************************************************

HsCD00404793      AGCAGCAGCTCATCGACGACCACTTCCTCTTCGACAAGCCCGTGTCGCCCCTGCTGCTGG
NM_001823.4       AGCAGCAGCTCATCGACGACCACTTCCTCTTCGACAAGCCCGTGTCGCCCCTGCTGCTGG
                  ************************************************************

HsCD00404793      CCTCGGGCATGGCCCGCGACTGGCCCGACGCCCGCGGTATCTGGCACAATGACAATAAGA
NM_001823.4       CCTCGGGCATGGCCCGCGACTGGCCCGACGCCCGCGGTATCTGGCACAATGACAATAAGA
                  ************************************************************

HsCD00404793      CCTTCCTGGTGTGGGTCAACGAGGAGGACCACCTGCGGGTCATCTCCATGCAGAAGGGGG
NM_001823.4       CCTTCCTGGTGTGGGTCAACGAGGAGGACCACCTGCGGGTCATCTCCATGCAGAAGGGGG
                  ************************************************************

HsCD00404793      GCAACATGAAGGAGGTGTTCACCCGCTTCTGCACCGGCCTCACCCAGATTGAAACTCTCT
NM_001823.4       GCAACATGAAGGAGGTGTTCACCCGCTTCTGCACCGGCCTCACCCAGATTGAAACTCTCT
                  ************************************************************

HsCD00404793      TCAAGTCTAAGGACTATGAGTTCATGTGGAACCCTCACCTGGGCTACATCCTCACCTGCC
NM_001823.4       TCAAGTCTAAGGACTATGAGTTCATGTGGAACCCTCACCTGGGCTACATCCTCACCTGCC
                  ************************************************************

HsCD00404793      CATCCAACCTGGGCACCGGGCTGCGGGCAGGTGTGCATATCAAGCTGCCCAACCTGGGCA
NM_001823.4       CATCCAACCTGGGCACCGGGCTGCGGGCAGGTGTGCATATCAAGCTGCCCAACCTGGGCA
                  ************************************************************

HsCD00404793      AGCATGAGAAGTTCTCGGAGGTGCTTAAGCGGCTGCGACTTCAGAAGCGAGGCACAGGCG
NM_001823.4       AGCATGAGAAGTTCTCGGAGGTGCTTAAGCGGCTGCGACTTCAGAAGCGAGGCACAGGCG
                  ************************************************************

HsCD00404793      GTGTGGACACGGCTGCGGTGGGCGGGGTCTTCGACGTCTCCAACGCTGACCGCCTGGGCT
NM_001823.4       GTGTGGACACGGCTGCGGTGGGCGGGGTCTTCGACGTCTCCAACGCTGACCGCCTGGGCT
                  ************************************************************

HsCD00404793      TCTCAGAGGTGGAGCTGGTGCAGATGGTGGTGGACGGAGTGAAGCTGCTCATCGAGATGG
NM_001823.4       TCTCAGAGGTGGAGCTGGTGCAGATGGTGGTGGACGGAGTGAAGCTGCTCATCGAGATGG
                  ************************************************************

HsCD00404793      AACAGCGGCTGGAGCAGGGCCAGGCCATCGACGACCTCATGCCTGCCCAGAAATGCCCAA
NM_001823.4       AGCAGCGGCTGGAGCAGGGCCAGGCCATCGACGACCTCATGCCTGCCCAGAAATGA----
                  *.*****************************************************.    

HsCD00404793      CTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_001823.4       ---------------------------------------------
                                                               

Pathways

Reactome : Creatine metabolism
Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
WikiPathway : Urea cycle and metabolism of amino groups

Protein

HPRD : 00423

Clone

Cloning Information : 580222

Organism


TAX_ID : 9606
Species Specific ID: 1152

Genome

Chromosome : 14
Map Location : 14q32
Ensembl : ENSG00000166165

Reagents

Labome : CKB-antibody