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FRAT2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FRAT2
Gene Name:  frequently rearranged in advanced T-cell lymphomas 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH187851.01L
Keyword: STOP codon removed based on OC annotation
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00041307

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 699nts        
Open reading frame : 1 to 699
Reference Sequence Alignment

ATGCCGTGCCGGAGGGAGGAGGAAGAGGAAGCCGGCGAGGAGGCGGAGGGGGAGGAAGAG
GAGGACGACAGCTTCCTCCTGCTGCAGCAGTCGGTGACGCTGGGCAGCTCGGGCGAGGTG
GACCGGCTGGTGGCCCAGATCGGCGAGACGCTGCAGCTGGACGCGGCGCAGGACAGCCCG
GCCTCGCCGTGCGCGCCCCCGGGGGTGCCGCTGCGGGCCCCGGGGCCCCTGGCTGCGGCG
GTGCCGACGGACAAGGCCCGGCCCCCGGCGGTGCCGCTGCTGCTGCCGCCCGCTTCGGCT
GAGACGGTGGGCCCGGCGCCCTCTGGGGCCCTGCGCTGCGCCCTAGGGGACCGCGGCCGC
GTGCGCGGACGCGCTGCGCCCTACTGCGTGGCGGAGGTCGCCGCAGGCCCCAGCGCGCTG
CCGGGGCCGTGCCGGCGAGGATGGCTCAGGGACGCGGTCACCTCCCGCCGCTTGCAGCAG
CGCCGATGGACCCAAGCCGGGGCACGCGCCGGCGACGACGACCCGCATCGGCTCCTCCAG
CAGCTCGTGCTCTCGGGAAACCTCATCAAGGAAGCCGTGCGGAGACTCCAACGAGCCGTC
GCCGCGGTTGCAGCCACGGGCCCCGCAAGCGCCCCTGGGCCCGGGGGAGGCCGCAGCGGA
CCTGACCGCATTGCCCTGCAGCCCTCAGGCTCCTTGCTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 105
End on reference sequence 806
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site U19 primer 517 535
primer site Att P2 Primer 3026 3043
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FRAT2
Symbol Nomenclature : FRAT2
Designation : FRAT-2|GSK-3 binding protein FRAT2|GSK-3-binding protein FRAT2
Full Nomenclature : frequently rearranged in advanced T-cell lymphomas 2
GENEID : 23401
Locus Tag : RP11-452K12.2
HGNC : 16048
MIM : 605006
Vega : OTTHUMG00000018850
Target GenBank: BC020165

 

Reference Sequence Alignment

  

HsCD00041307      ATGCCGTGCCGGAGGGAGGAGGAAGAGGAAGCCGGCGAGGAGGCGGAGGGGGAGGAAGAG
NM_012083.2       ATGCCGTGCCGGAGGGAGGAGGAAGAGGAAGCCGGCGAGGAGGCGGAGGGGGAGGAAGAG
                  ************************************************************

HsCD00041307      GAGGACGACAGCTTCCTCCTGCTGCAGCAGTCGGTGACGCTGGGCAGCTCGGGCGAGGTG
NM_012083.2       GAGGACGACAGCTTCCTCCTGCTGCAGCAGTCGGTGACGCTGGGCAGCTCGGGCGAGGTG
                  ************************************************************

HsCD00041307      GACCGGCTGGTGGCCCAGATCGGCGAGACGCTGCAGCTGGACGCGGCGCAGGACAGCCCG
NM_012083.2       GACCGGCTGGTGGCCCAGATCGGCGAGACGCTGCAGCTGGACGCGGCGCAGGACAGCCCG
                  ************************************************************

HsCD00041307      GCCTCGCCGTGCGCGCCCCCGGGGGTGCCGCTGCGGGCCCCGGGGCCCCTGGCTGCGGCG
NM_012083.2       GCCTCGCCGTGCGCGCCCCCGGGGGTGCCGCTGCGGGCCCCGGGGCCCCTGGCTGCGGCG
                  ************************************************************

HsCD00041307      GTGCCGACGGACAAGGCCCGGCCCCCGGCGGTGCCGCTGCTGCTGCCGCCCGCTTCGGCT
NM_012083.2       GTGCCGGCGGACAAGGCCCGGCCCCCGGCGGTGCCGCTGCTGCTGCCGCCCGCTTCGGCT
                  ******.*****************************************************

HsCD00041307      GAGACGGTGGGCCCGGCGCCCTCTGGGGCCCTGCGCTGCGCCCTAGGGGACCGCGGCCGC
NM_012083.2       GAGACGGTGGGCCCGGCGCCCTCTGGGGCCCTGCGCTGCGCCCTAGGGGACCGCGGCCGC
                  ************************************************************

HsCD00041307      GTGCGCGGACGCGCTGCGCCCTACTGCGTGGCGGAGGTCGCCGCAGGCCCCAGCGCGCTG
NM_012083.2       GTGCGCGGACGCGCTGCGCCCTACTGCGTGGCGGAGGTCGCCGCAGGCCCCAGCGCGCTG
                  ************************************************************

HsCD00041307      CCGGGGCCGTGCCGGCGAGGATGGCTCAGGGACGCGGTCACCTCCCGCCGCTTGCAGCAG
NM_012083.2       CCGGGGCCGTGCCGGCGAGGATGGCTCAGGGACGCGGTCACCTCCCGCCGCTTGCAGCAG
                  ************************************************************

HsCD00041307      CGCCGATGGACCCAAGCCGGGGCACGCGCCGGCGACGACGACCCGCATCGGCTCCTCCAG
NM_012083.2       CGCCGATGGACCCAAGCCGGGGCACGCGCCGGCGACGACGACCCGCATCGGCTCCTCCAG
                  ************************************************************

HsCD00041307      CAGCTCGTGCTCTCGGGAAACCTCATCAAGGAAGCCGTGCGGAGACTCCAACGAGCCGTC
NM_012083.2       CAGCTCGTGCTCTCGGGAAACCTCATCAAGGAAGCCGTGCGGAGACTCCAACGAGCCGTC
                  ************************************************************

HsCD00041307      GCCGCGGTTGCAGCCACGGGCCCCGCAAGCGCCCCTGGGCCCGGGGGAGGCCGCAGCGGA
NM_012083.2       GCCGCGGTTGCAGCCACGGGCCCCGCAAGCGCCCCTGGGCCCGGGGGAGGCCGCAGCGGA
                  ************************************************************

HsCD00041307      CCTGACCGCATTGCCCTGCAGCCCTCAGGCTCCTTGCTCTTG
NM_012083.2       CCTGACCGCATTGCCCTGCAGCCCTCAGGCTCCTTGCTCTGA
                  **************************************** .

Pathways

Reactome : Beta-catenin phosphorylation cascade
Reactome : Degradation of beta-catenin by the destruction complex
Reactome : disassembly of the destruction complex and recruitment of AXIN to the membrane
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : TCF dependent signaling in response to WNT

Protein

UniProt : O75474
HPRD : 05419

Clone

Cloning Information : 187851
HIP Master Clone ID : 162795
Original Clone ID : FLH187851.01L

Organism


TAX_ID : 9606
Species Specific ID: 23401

Genome

Chromosome : 10
Map Location : 10q24.1
Ensembl : ENSG00000181274

Reagents

Labome : FRAT2-antibody