DNASU Plasmid | ALG10 (Homo sapiens) in pGEc1-DEST

Explanation of Terms

Gene:

Gene Symbol: ALG10B

Gene Name: ALG10B, alpha-1,2-glucosyltransferase

Original Clone ID:

HGT59_M01

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pGEc1-DEST

Format:

FUSION

Source:

Glyco-enzyme clone collection

Description:

All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen

HsCD00413753

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 1419nts

Open reading frame: 1 to 1419

Reference Sequence Alignment

ATGGCGCAGCTGGAAGGTTACTATTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTATCC
TGCCTCCTCTTCTCCGCCTTCAGCCGGGCGTTGCGAGAGCCCTACATGGACGAGATCTTC
CACCTGCCTCAGGCGCAGCGCTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCC
ATGATTACTACATTACCTGGCTTGTACCTGGTGTCAATTGGAGTGATCAAACCTGCCATT
TGGATCTTTGGATGGTCTGAACATGTTGTCTGCTCCATTGGGATGCTCAGATTTGTTAAT
CTTCTCTTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCTGCAAGGTACAACCC
AGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTT
CCAACACTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACT
CTTTTTGCGTATTTGATGTGTCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTT
TGTGGCTTCGTGTTTCGGCAAACAAATATCATCTGGGCTGTCTTCTGTGCAGGAAATGTC
ATTGCACAAAAGTTAACGGAGGCTTGGAAAACTGAGCTACAAAAGAAGGAAGACAGACTT
CCACCTATTAAAGGACCATTTGCAGAATTCAGAAAAATTCTTCAGTTTCTTTTGGCTTAT
TCCATGTCCTTTAAAAACTTGAGTATGCTTTTGCTTCTGACTTGGCCCTACATCCTTCTG
GGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGT
AGTCATGAAGCCTGTCTTCATTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTT
TTTTCCTTTCCTCATCTCCTGTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGG
AAACGTAGAATTCTGTTTTTTGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTC
ACTTATGCTCATAAATACTTGCTAGCAGACAATAGACATTATACTTTCTATGTGTGGAAA
AGAGTTTTTCAAAGATATGAAACTGTAAAATATTTGTTAGTTCCAGCCTATATATTTGCT
GGTTGGAGTATAGCTGACTCATTGAAATCAAAGTCAATTTTTTGGAATTTAATGTTTTTC
ATATGCTTGTTCACTGTTATAGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTA
CCTTATGTCATTTATAGGCTTAACATACCTCTGCCTCCCACATCCAGACTCATTTGTGAA
CTGAGCTGCTATGCAGTTGTTAATTTCATAACTTTTTTCATCTTTCTGAACAAGACTTTT
CAGTGGCCAAATAGTCAGGACATTCAAAGGTTTATGTGG

Protein sequence predicted based on nucleotide sequence

MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGHFSLSQWDP
MITTLPGLYLVSIGVIKPAIWIFGWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFCKVQP
RNKAASSIQRVLSTLTLAVFPTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGF
CGFVFRQTNIIWAVFCAGNVIAQKLTEAWKTELQKKEDRLPPIKGPFAEFRKILQFLLAY
SMSFKNLSMLLLLTWPYILLGFLFCAFVVVNGGIVIGDRSSHEACLHFPQLFYFFSFTLF
FSFPHLLSPSKIKTFLSLVWKRRILFFVVTLVSVFLVWKFTYAHKYLLADNRHYTFYVWK
RVFQRYETVKYLLVPAYIFAGWSIADSLKSKSIFWNLMFFICLFTVIVPQKLLEFRYFIL
PYVIYRLNIPLPPTSRLICELSCYAVVNFITFFIFLNKTFQWPNSQDIQRFMW

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pGEc1-DEST

Synonyms:

pXLG-CtermHisStrep-DEST

Sequencing Primer:

Forward: pGEc1-DEST F

Reverse: pGEn1-DEST R

Description:

Mammalian expression vector with a CMV promoter and C-terminal 8xHis and Strep tags; ampicillin resistance in bacteria; Gateway recombinational cloning

Comments:

http://glycoenzymes.ccrc.uga.edu/

Size (bp):

6126

Parent Vector:

None

Properties:

Gateway, acceptor (destination), mammalian expression, recombinational cloning, with tag/fusion/marker

Author Name:

University of Georgia
Kelley Moremen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCATTAGGCACCCCAGGCTTTACCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAGAGCGCCCAATACGCAAGGAAACAGCTATGACCATGTTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGGAAGGCCCATGAGGCCAGTTAATTAAGAGGTACCTTAATTAAGGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCGCAATTCGCCCTTAAGCTAGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCTGCAGAAGTTGGTCGTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCAGCGGCCATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGCCGCCATCATCATCATCATCATCATCATGCGTGGAGCCACCCGCAGTTCGAAAAATAATAAGGATCCAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGAGATCTGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACTCGAGTTAAGGGCGAATTGCCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCCTTAATTAAGAGCTCATGGCGCGCCTAGGCCTTGACGGCCTTCCGCCAATTCGCCCTATAGTGAGTCGTATTACGTCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAAACGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGAGCGCCCTGTAGCGGCCACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

Col E1 bacterial origin

1137

1804

death cassette

ccdB

ccdB death cassette (removed in plasmids with insert)

4211

4516

intron

artificial intron

2828

2960

poly-A signal

BGH pA

bovine growth hormone (BGH) polyadenylation signal

5302

5516

promoter

CMV

CMV promoter

2208

2790

recombination site

3'-ITR

inverted terminal repeat sequence

5604

5733

recombination site

5'ITR

inverted terminal repeat sequence

1998

2127

recombination site

attR1

attR1 site

2974

3101

recombination site

attR2

attR2 site

4557

4681

selectable marker

AmpR

ampicillin resistance gene

129

989

selectable marker

CmR

Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)

3210

2859

tag

His

C-terminal 8xHis tag

4691

4714

tag

strep

strep tag

4718

4741

trxn regulatory element

WPRE

woodchuck hepatitis post-transcriptional regulatory element

4754

5295

Gene

Gene Symbol :

ALG10B

Symbol Nomenclature :

ALG10B

SYNONYM :

ALG10; KCR1

Designation :

alpha-1,2-glucosyltransferase ALG10-A|alpha-2-glucosyltransferase ALG10-B|asparagine-linked glycosylation 10 homolog B (yeast, alpha-1,2-glucosyltransferase)|asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog B|asparagine-linked glycosylation protein 10 homolog B|dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase|modifier of the HERG potassium channel|potassium channel regulator 1|putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase|putative alpha-1,2-glucosyltransferase ALG10-B

Full Nomenclature :

ALG10B, alpha-1,2-glucosyltransferase

GENEID :

144245

HGNC :

31088

Target GenBank:

BC070347

Reference Sequence Alignment

  

HsCD00413753        ATGGCGCAGCTGGAAGGTTACTATTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTATCC
NM_032834.3         ATGGCGCAGCTGGAAGGTTACTATTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTATCC
NM_001013620.3      ATGGCGCAGCTAGAGGGTTACTGTTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTGTCC
                    ***********.**.*******.*********************************.***

HsCD00413753        TGCCTCCTCTTCTCCGCCTTCAGCCGGGCGTTGCGAGAGCCCTACATGGACGAGATCTTC
NM_032834.3         TGCCTCCTCTTCTCCGCCTTCAGCCGGGCGTTGCGAGAGCCCTACATGGACGAGATCTTC
NM_001013620.3      TGCCTCCTCTTCTCCGCCTTCAGCCGGGCGCTGCGAGAGCCCTACATGGACGAGATCTTC
                    ****************************** *****************************

HsCD00413753        CACCTGCCTCAGGCGCAGCGCTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCC
NM_032834.3         CACCTGCCTCAGGCGCAGCGCTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCC
NM_001013620.3      CACCTGCCTCAGGCGCAGCGCTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCC
                    ************************************************************

HsCD00413753        ATGATTACTACATTACCTGGCTTGTACCTGGTGTCAATTGGAGTGATCAAACCTGCCATT
NM_032834.3         ATGATTACTACATTACCTGGCTTGTACCTGGTGTCAATTGGAGTGATCAAACCTGCCATT
NM_001013620.3      ATGATTACTACATTACCTGGCTTGTACCTGGTGTCAGTTGGAGTGGTCAAACCTGCCATT
                    ************************************.********.**************

HsCD00413753        TGGATCTTTGGATGGTCTGAACATGTTGTCTGCTCCATTGGGATGCTCAGATTTGTTAAT
NM_032834.3         TGGATCTTTGGATGGTCTGAACATGTTGTCTGCTCCATTGGGATGCTCAGATTTGTTAAT
NM_001013620.3      TGGATCTTTGGATGGTCTGAACATGTTGTCTGCTCCATTGGGATGCTCAGATTTGTTAAT
                    ************************************************************

HsCD00413753        CTTCTCTTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCTGCAAGGTACAACCC
NM_032834.3         CTTCTCTTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCTGCAAGGTACAACCC
NM_001013620.3      CTTCTCTTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCCACAAGGTACAACCC
                    ********************************************* .*************

HsCD00413753        AGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTT
NM_032834.3         AGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTT
NM_001013620.3      AGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTT
                    ************************************************************

HsCD00413753        CCAACACTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACT
NM_032834.3         CCAACACTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACT
NM_001013620.3      CCAACACTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACT
                    ************************************************************

HsCD00413753        CTTTTTGCGTATTTGATGTGTCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTT
NM_032834.3         CTTTTTGCGTATTTGATGTGTCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTT
NM_001013620.3      CTTTTTGCATATTTGATGTGTCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTT
                    ********.***************************************************

HsCD00413753        TGTGGCTTCGTGTTTCGGCAAACAAATATCATCTGGGCTGTCTTCTGTGCAGGAAATGTC
NM_032834.3         TGTGGCTTCATGTTTCGGCAAACAAATATCATCTGGGCTGTCTTCTGTGCAGGAAATGTC
NM_001013620.3      TGTGGCTTCATGTTTCGGCAAACAAATATCATCTGGGCTGTCTTCTGTGCAGGGAATGTC
                    *********.*******************************************.******

HsCD00413753        ATTGCACAAAAGTTAACGGAGGCTTGGAAAACTGAGCTACAAAAGAAGGAAGACAGACTT
NM_032834.3         ATTGCACAAAAGTTAACGGAGGCTTGGAAAACTGAGCTACAAAAGAAGGAAGACAGACTT
NM_001013620.3      ATTGCACAAAAGTTAACTGAGGCTTGGAAAACTGAGCTACAAAAGAAGGAAGACAGACTT
                    ***************** ******************************************

HsCD00413753        CCACCTATTAAAGGACCATTTGCAGAATTCAGAAAAATTCTTCAGTTTCTTTTGGCTTAT
NM_032834.3         CCACCTATTAAAGGACCATTTGCAGAATTCAGAAAAATTCTTCAGTTTCTTTTGGCTTAT
NM_001013620.3      CCACCTATTAAAGGACCATTTGCAGAATTCAGAAAAATTCTTCAGTTTCTTTTGGCTTAT
                    ************************************************************

HsCD00413753        TCCATGTCCTTTAAAAACTTGAGTATGCTTTTGCTTCTGACTTGGCCCTACATCCTTCTG
NM_032834.3         TCCATGTCCTTTAAAAACTTGAGTATGCTTTTGCTTCTGACTTGGCCCTACATCCTTCTG
NM_001013620.3      TCCATGTCCTTTAAAAACTTGAGTATGCTTTTCTGTTTGACTTGGCCCTACATCCTTCTG
                    ********************************   * ***********************

HsCD00413753        GGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGT
NM_032834.3         GGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGT
NM_001013620.3      GGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGT
                    ************************************************************

HsCD00413753        AGTCATGAAGCCTGTCTTCATTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTT
NM_032834.3         AGTCATGAAGCCTGTCTTCATTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTT
NM_001013620.3      AGTCATGAAGCCTGTCTTCATTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTT
                    ************************************************************

HsCD00413753        TTTTCCTTTCCTCATCTCCTGTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGG
NM_032834.3         TTTTCCTTTCCTCATCTCCTGTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGG
NM_001013620.3      TTTTCTTTTCCTCATCTCCTGTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGG
                    ***** ******************************************************

HsCD00413753        AAACGTAGAATTCTGTTTTTTGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTC
NM_032834.3         AAACGTAGAATTCTGTTTTTTGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTC
NM_001013620.3      AAACATGGAATTCTGTTTTTGGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTC
                    ****.*.************* ***************************************

HsCD00413753        ACTTATGCTCATAAATACTTGCTAGCAGACAATAGACATTATACTTTCTATGTGTGGAAA
NM_032834.3         ACTTATGCTCATAAATACTTGCTAGCAGACAATAGACATTATACTTTCTATGTGTGGAAA
NM_001013620.3      ACTTATGCTCATAAATACTTGCTAGCAGACAATAGACATTATACTTTCTATGTGTGGAAA
                    ************************************************************

HsCD00413753        AGAGTTTTTCAAAGATATGAAACTGTAAAATATTTGTTAGTTCCAGCCTATATATTTGCT
NM_032834.3         AGAGTTTTTCAAAGATATGAAACTGTAAAATATTTGTTAGTTCCAGCCTATATATTTGCT
NM_001013620.3      AGAGTTTTTCAAAGATATGCAATTCTGAAATATTTGTTAGTTCCAGCCTATATATTTGCT
                    *******************.** * *.*********************************

HsCD00413753        GGTTGGAGTATAGCTGACTCATTGAAATCAAAGTCAATTTTTTGGAATTTAATGTTTTTC
NM_032834.3         GGTTGGAGTATAGCTGACTCATTGAAATCAAAGTCAATTTTTTGGAATTTAATGTTTTTC
NM_001013620.3      GGTTGGAGTATAGCTGACTCATTGAAATCAAAGCCAATTTTTTGGAATTTAATGTTTTTC
                    ********************************* **************************

HsCD00413753        ATATGCTTGTTCACTGTTATAGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTA
NM_032834.3         ATATGCTTGTTCACTGTTATAGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTA
NM_001013620.3      ATATGCTTGTTCATTGTTATAGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTA
                    ************* **********************************************

HsCD00413753        CCTTATGTCATTTATAGGCTTAACATACCTCTGCCTCCCACATCCAGACTCATTTGTGAA
NM_032834.3         CCTTATGTCATTTATAGGCTTAACATACCTCTGCCTCCCACATCCAGACTCATTTGTGAA
NM_001013620.3      CCTTATGTCATTTATAGGCTTAACATAACTCTGCCTCCCACATCCAGACTTGTTTGTGAA
                    ***************************.********************** .********

HsCD00413753        CTGAGCTGCTATGCAGTTGTTAATTTCATAACTTTTTTCATCTTTCTGAACAAGACTTTT
NM_032834.3         CTGAGCTGCTATGCAGTTGTTAATTTCATAACTTTTTTCATCTTTCTGAACAAGACTTTT
NM_001013620.3      CTGAGTTGCTATGCAATTGTTAATTTCATAACTTTTTACATCTTTCTGAACAAGACTTTT
                    ***** *********.*********************:**********************

HsCD00413753        CAGTGGCCAAATAGTCAGGACATTCAAAGGTTTATGTGG---
NM_032834.3         CAGTGGCCAAATAGTCAGGACATTCAAAGGTTTATGTGGTAA
NM_001013620.3      CAGTGGCCAAATAGTCAGGACATTCAAAGGTTTATGTGGTAA
                    ***************************************

Pathways

Reactome :

Asparagine N-linked glycosylation

Reactome :

Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

Reactome :

Metabolism of proteins

Reactome :

Post-translational protein modification

Protein

HPRD :

11923

Clone

Cloning Information :

582413

Original Clone ID :

HGT59_M01

Organism

Species Specific ID:

84920

Genome

Chromosome :

Map Location :

12q12

Reagents

Labome :

ALG10-antibody