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MPG (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MPG
Gene Name:  N-methylpurine-DNA glycosylase
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH190258.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00041651

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 882nts         Open reading frame : 1 to 882
Reference Sequence Alignment

ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAAAAGAAGCAGCGACCA
GCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCACCTGCAGAGCAGCCA
CACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGCTGCTTGGGACCGCCC
ACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAGGGCCACCTTACCCGA
CTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGGGCATTTCTGGGACAG
GTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGCATCGTGGAGACCGAG
GCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGCCGGCAGACCCCCCGC
AACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATCATTTACGGCATGTAC
TTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTGCTGCGAGCACTGGAG
CCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTCCGGAAAGGCACCGCC
AGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAGCTGTGCCAGGCCCTG
GCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAAGCTGTATGGCTGGAG
CGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCCCGGGTGGGCGTCGGC
CATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGGGGCAGCCCCTGGGTC
AGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 961
Start on reference sequence 80
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MPG
Symbol Nomenclature : MPG
SYNONYM : AAG; ADPG; APNG; CRA36.1; MDG; Mid1; PIG11; PIG16; anpg
Designation : 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p|3-alkyladenine DNA glycosylase|3-methyladenine DNA glycosidase|CRA36.1 (3-methyl-adenine DNA glycosylase)|DNA-3-methyladenine glycosylase|N-methylpurine-DNA glycosylase, MPG|proliferation-inducing protein 11|proliferation-inducing protein 16
Full Nomenclature : N-methylpurine-DNA glycosylase
GENEID : 4350
Locus Tag : Z69720.1-002
HGNC : 7211
MIM : 156565
Vega : OTTHUMG00000047887
Target GenBank: BC014991

 

Reference Sequence Alignment

  

HsCD00041651        ---------------ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAA
NM_001015054.2      ---------------------------------------------------ATGGGGCAA
NM_001015052.2      ---------------ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAA
NM_002434.3         ATGGTCACCCCCGCTTTGCAGATGAAGAAACCAAAGCAGTTTTGCCGACGGATGGGGCAA
                                                                       *********

HsCD00041651        AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_001015054.2      AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_001015052.2      AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
NM_002434.3         AAGAAGCAGCGACCAGCTAGAGCAGGGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCA
                    ************************************************************

HsCD00041651        CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_001015054.2      CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_001015052.2      CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
NM_002434.3         CCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGCCCAGGCACCTTGCCCCAGGGAGCGC
                    ************************************************************

HsCD00041651        TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_001015054.2      TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_001015052.2      TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
NM_002434.3         TGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTATTTCTCAAGCCCAAAG
                    ************************************************************

HsCD00041651        GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_001015054.2      GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_001015052.2      GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
NM_002434.3         GGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGGCCCGG
                    ************************************************************

HsCD00041651        GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_001015054.2      GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_001015052.2      GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
NM_002434.3         GCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGC
                    ************************************************************

HsCD00041651        ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_001015054.2      ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_001015052.2      ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
NM_002434.3         ATCGTGGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGC
                    ************************************************************

HsCD00041651        CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_001015054.2      CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_001015052.2      CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
NM_002434.3         CGGCAGACCCCCCGCAACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATC
                    ************************************************************

HsCD00041651        ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_001015054.2      ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_001015052.2      ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
NM_002434.3         ATTTACGGCATGTACTTCTGCATGAACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTG
                    ************************************************************

HsCD00041651        CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_001015054.2      CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_001015052.2      CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
NM_002434.3         CTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGACCATGCGTCAGCTTCGCAGCACCCTC
                    ************************************************************

HsCD00041651        CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_001015054.2      CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_001015052.2      CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
NM_002434.3         CGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGCAGTGGCCCCTCCAAG
                    ************************************************************

HsCD00041651        CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_001015054.2      CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_001015052.2      CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
NM_002434.3         CTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGGATGAA
                    ************************************************************

HsCD00041651        GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_001015054.2      GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_001015052.2      GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
NM_002434.3         GCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCC
                    ************************************************************

HsCD00041651        CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_001015054.2      CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_001015052.2      CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
NM_002434.3         CGGGTGGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGG
                    ************************************************************

HsCD00041651        GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTTG
NM_001015054.2      GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
NM_001015052.2      GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
NM_002434.3         GGCAGCCCCTGGGTCAGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCCTGA
                    ******************************************************* .

Pathways

NCI : Validated nuclear estrogen receptor alpha network
Reactome : Base Excision Repair
Reactome : Base-Excision Repair, AP Site Formation
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : Cleavage of the damaged purine
Reactome : DNA Repair
Reactome : Depurination
Reactome : Displacement of DNA glycosylase by APE1
Reactome : Recognition and association of DNA glycosylase with site containing an affected purine
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
Reactome : Resolution of Abasic Sites (AP sites)

Protein

UniProt : Q1W6H1
UniProt : P29372
UniProt : Q5J9I4
HPRD : 06766

Clone

Cloning Information : 190258
HIP Master Clone ID : 165202
Original Clone ID : FLH190258.01L

Organism


TAX_ID : 9606
Species Specific ID: 4350

Genome

Chromosome : 16
Map Location : 16p13.3
Ensembl : ENSG00000103152

Reagents

Labome : MPG-antibody