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ARPM1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTRT3
Gene Name:  actin-related protein T3
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH194330.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00042138

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1119nts        
Open reading frame : 1 to 1119
Reference Sequence Alignment

ATGAACCACTGCCAGCTACCGGTGGTGATCGACAACGGCTCGGGAATGATCAAGGCGGGC
GTGGCTGGGTGCCGGGAGCCCCAGTTTATCTACCCGAACATTATCGGCCGCGCCAAGGGC
CAGAGCCGCGCGGCCCAGGGCGGGCTAGAACTCTGCGTGGGCGACCAAGCTCAGGACTGG
AGGAGCTCGCTGTTCATCAGTTACCCAGTGGAGCGTGGTCTCATTACTTCATGCGAGGAC
ATGGAGATCATGTGGAAGCATATCTATGACTATAACCTAAAGCTGAAGCCGTGTGATGGC
CCAGTCTTGATTACTGAGCCAGCGCTGAACCCACTGGCCAACCGGCAACAGATCACGGAA
ATGTTTTTTGAGCATCTGGGTGTTCCTGCCTTCTATATGTCCATCCAGGCTGTGCTGGCT
CTCTTTGCTGCTGGCTTCACTACTGGCCTTGTGCTGAATTCAGGTGCTGGGGTTACCCAG
AGTGTGCCCATCTTTGAGGGTTACTGTCTGCCTCATGGTGTGCAGCAACTGGATCTGGCA
GGCCTTGACCTCACCAACTACCTCATGGTGCTAATGAAGAACCATGGTATCATGTTGCTC
AGTGCTTCAGACAGAAAGATTGTTGAAGACATCAAGGAGAGCTTTTGTTATGTGGCAATG
AACTACGAAGAGGAAATGGCCAAGAAACCCGATTGTCTAGAGAAAGTTTACCAACTACCT
GATGGGAAGGTCATCCAGCTCCATGACCAGCTCTTTTCTTGTCCAGAGGCCCTCTTCTCT
CCGTGTCATATGAACCTTGAGGCCCCTGGCATTGATAAGATATGCTTCAGCAGCATAATG
AAATGTGATACAGGCCTGAGGAATTCCTTCTTTTCCAATATTATCCTTGCCGGGGGATCA
ACCTCTTTCCCTGGTTTAGACAAGCGGTTAGTTAAGGATATAGCAAAGGTGGCTCCTGCC
AACACCGCTGTGCAAGTTATAGCTCCTCCAGAAAGGAAAATATCAGTGTGGATGGGAGGT
TCTATTCTTGCATCCTTGTCTGCCTTCCAGGACATGTGGATCACTGCTGCAGAATTTAAA
GAAGTTGGACCCAACATAGTACACCAAAGATGCTTCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1414
Start on reference sequence 296
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site U19 primer 517 535
primer site Att P2 Primer 3026 3043
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTRT3
Symbol Nomenclature : ACTRT3
SYNONYM : ARP-T3; ARPM1
Designation : actin related protein M1|actin-related protein M1
Full Nomenclature : actin-related protein T3
GENEID : 84517
HGNC : 24022
MIM : 608534
Vega : OTTHUMG00000184025
Target GenBank: BC007289

 

Reference Sequence Alignment

  

HsCD00042138      ATGAACCACTGCCAGCTACCGGTGGTGATCGACAACGGCTCGGGAATGATCAAGGCGGGC
NM_032487.4       ATGAACCACTGCCAGCTACCGGTGGTGATCGACAACGGCTCGGGAATGATCAAGGCGGGC
                  ************************************************************

HsCD00042138      GTGGCTGGGTGCCGGGAGCCCCAGTTTATCTACCCGAACATTATCGGCCGCGCCAAGGGC
NM_032487.4       GTGGCTGGGTGCCGGGAGCCCCAGTTTATCTACCCGAACATTATCGGCCGCGCCAAGGGC
                  ************************************************************

HsCD00042138      CAGAGCCGCGCGGCCCAGGGCGGGCTAGAACTCTGCGTGGGCGACCAAGCTCAGGACTGG
NM_032487.4       CAGAGCCGCGCGGCCCAGGGCGGGCTAGAACTCTGCGTGGGCGACCAAGCTCAGGACTGG
                  ************************************************************

HsCD00042138      AGGAGCTCGCTGTTCATCAGTTACCCAGTGGAGCGTGGTCTCATTACTTCATGCGAGGAC
NM_032487.4       AGGAGCTCGCTGTTCATCAGTTACCCAGTGGAGCGTGGTCTCATTACTTCATGGGAGGAC
                  ***************************************************** ******

HsCD00042138      ATGGAGATCATGTGGAAGCATATCTATGACTATAACCTAAAGCTGAAGCCGTGTGATGGC
NM_032487.4       ATGGAGATCATGTGGAAGCATATCTATGACTATAACCTAAAGCTGAAGCCGTGTGATGGC
                  ************************************************************

HsCD00042138      CCAGTCTTGATTACTGAGCCAGCGCTGAACCCACTGGCCAACCGGCAACAGATCACGGAA
NM_032487.4       CCAGTCTTGATTACTGAGCCAGCGCTGAACCCACTGGCCAACCGGCAACAGATCACGGAA
                  ************************************************************

HsCD00042138      ATGTTTTTTGAGCATCTGGGTGTTCCTGCCTTCTATATGTCCATCCAGGCTGTGCTGGCT
NM_032487.4       ATGTTTTTTGAGCATCTGGGTGTTCCTGCCTTCTATATGTCCATCCAGGCTGTGCTGGCT
                  ************************************************************

HsCD00042138      CTCTTTGCTGCTGGCTTCACTACTGGCCTTGTGCTGAATTCAGGTGCTGGGGTTACCCAG
NM_032487.4       CTCTTTGCTGCTGGCTTCACTACTGGCCTTGTGCTGAATTCAGGTGCTGGGGTTACCCAG
                  ************************************************************

HsCD00042138      AGTGTGCCCATCTTTGAGGGTTACTGTCTGCCTCATGGTGTGCAGCAACTGGATCTGGCA
NM_032487.4       AGTGTGCCCATCTTTGAGGGTTACTGTCTGCCTCATGGTGTGCAGCAACTGGATCTGGCA
                  ************************************************************

HsCD00042138      GGCCTTGACCTCACCAACTACCTCATGGTGCTAATGAAGAACCATGGTATCATGTTGCTC
NM_032487.4       GGCCTTGACCTCACCAACTACCTCATGGTGCTAATGAAGAACCATGGTATCATGTTGCTC
                  ************************************************************

HsCD00042138      AGTGCTTCAGACAGAAAGATTGTTGAAGACATCAAGGAGAGCTTTTGTTATGTGGCAATG
NM_032487.4       AGTGCTTCAGACAGAAAGATTGTTGAAGACATCAAGGAGAGCTTTTGTTATGTGGCAATG
                  ************************************************************

HsCD00042138      AACTACGAAGAGGAAATGGCCAAGAAACCCGATTGTCTAGAGAAAGTTTACCAACTACCT
NM_032487.4       AACTACGAAGAGGAAATGGCCAAGAAACCCGATTGTCTAGAGAAAGTTTACCAACTACCT
                  ************************************************************

HsCD00042138      GATGGGAAGGTCATCCAGCTCCATGACCAGCTCTTTTCTTGTCCAGAGGCCCTCTTCTCT
NM_032487.4       GATGGGAAGGTCATCCAGCTCCATGACCAGCTCTTTTCTTGTCCAGAGGCCCTCTTCTCT
                  ************************************************************

HsCD00042138      CCGTGTCATATGAACCTTGAGGCCCCTGGCATTGATAAGATATGCTTCAGCAGCATAATG
NM_032487.4       CCGTGTCATATGAACCTTGAGGCCCCTGGCATTGATAAGATATGCTTCAGCAGCATAATG
                  ************************************************************

HsCD00042138      AAATGTGATACAGGCCTGAGGAATTCCTTCTTTTCCAATATTATCCTTGCCGGGGGATCA
NM_032487.4       AAATGTGATACAGGCCTGAGGAATTCCTTCTTTTCCAATATTATCCTTGCCGGGGGATCA
                  ************************************************************

HsCD00042138      ACCTCTTTCCCTGGTTTAGACAAGCGGTTAGTTAAGGATATAGCAAAGGTGGCTCCTGCC
NM_032487.4       ACCTCTTTCCCTGGTTTAGACAAGCGGTTAGTTAAGGATATAGCAAAGGTGGCTCCTGCC
                  ************************************************************

HsCD00042138      AACACCGCTGTGCAAGTTATAGCTCCTCCAGAAAGGAAAATATCAGTGTGGATGGGAGGT
NM_032487.4       AACACCGCTGTGCAAGTTATAGCTCCTCCAGAAAGGAAAATATCAGTGTGGATGGGAGGT
                  ************************************************************

HsCD00042138      TCTATTCTTGCATCCTTGTCTGCCTTCCAGGACATGTGGATCACTGCTGCAGAATTTAAA
NM_032487.4       TCTATTCTTGCATCCTTGTCTGCCTTCCAGGACATGTGGATCACTGCTGCAGAATTTAAA
                  ************************************************************

HsCD00042138      GAAGTTGGACCCAACATAGTACACCAAAGATGCTTCTTG
NM_032487.4       GAAGTTGGACCCAACATAGTACACCAAAGATGCTTCTGA
                  ************************************* .

Protein

SMART domain : ACTIN : Actin
UniProt : Q9BYD9
HPRD : 12253

Clone

Cloning Information : 194330
HIP Master Clone ID : 169041
Original Clone ID : FLH194330.01L

Organism


TAX_ID : 9606
Species Specific ID: 84517

Genome

Chromosome : 3
Map Location : 3q26.2
Ensembl : ENSG00000184378

Reagents

Labome : ACTRT3-antibody