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IL18 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  IL18
Gene Name:  interleukin 18 (interferon-gamma-inducing factor)
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH021713.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00000423

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 582nts         Open reading frame : 1 to 582
Reference Sequence Alignment

ATGGCTGCTGAACCAGTAGAAGACAATTGCATCAACTTTGTGGCAATGAAATTTATTGAC
AATACGCTTTACTTTATAGCTGAAGATGATGAAAACCTGGAATCAGATTACTTTGGCAAG
CTTGAATCTAAATTATCAGTCATAAGAAATTTGAATGACCAAGTTCTCTTCATTGACCAA
GGAAATCGGCCTCTATTTGAAGATATGACTGATTCTGACTGTAGAGATAATGCACCCCGG
ACCATATTTATTATAAGTATGTATAAAGATAGCCAGCCTAGAGGTATGGCTGTAACTATC
TCTGTGAAGTGTGAGAAAATTTCAACTCTCTCCTGTGAGAACAAAATTATTTCCTTTAAG
GAAATGAATCCTCCTGATAACATCAAGGATACAAAAAGTGACATCATATTCTTTCAGAGA
AGTGTCCCAGGACATGATAATAAGATGCAATTTGAATCTTCATCATACGAAGGATACTTT
CTAGCTTGTGAAAAAGAGAGAGACCTTTTTAAACTCATTTTGAAAAAAGAGGATGAATTG
GGGGATAGATCTATAATGTTCACTGTTCAAAACGAAGACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 759
Start on reference sequence 178
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IL18
Symbol Nomenclature : IL18
SYNONYM : IGIF; IL-18; IL-1g; IL1F4
Designation : IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin-1 gamma|interleukin-18
Full Nomenclature : interleukin 18 (interferon-gamma-inducing factor)
GENEID : 3606
GI : 61365702
GenBank Accession : AY890809
HGNC : 5986
MIM : 600953
Vega : OTTHUMG00000167006
Target GenBank: NM_001562

 

Reference Sequence Alignment

  

HsCD00000423        ATGGCTGCTGAACCAGTAGAAGACAATTGCATCAACTTTGTGGCAATGAAATTTATTGAC
NM_001562.3         ATGGCTGCTGAACCAGTAGAAGACAATTGCATCAACTTTGTGGCAATGAAATTTATTGAC
NM_001243211.1      ATGGCTGCTGAACCAGTAGAAGACAATTGCATCAACTTTGTGGCAATGAAATTTATTGAC
                    ************************************************************

HsCD00000423        AATACGCTTTACTTTATAGCTGAAGATGATGAAAACCTGGAATCAGATTACTTTGGCAAG
NM_001562.3         AATACGCTTTACTTTATAGCTGAAGATGATGAAAACCTGGAATCAGATTACTTTGGCAAG
NM_001243211.1      AATACGCTTTACTTTATAG------------AAAACCTGGAATCAGATTACTTTGGCAAG
                    *******************            *****************************

HsCD00000423        CTTGAATCTAAATTATCAGTCATAAGAAATTTGAATGACCAAGTTCTCTTCATTGACCAA
NM_001562.3         CTTGAATCTAAATTATCAGTCATAAGAAATTTGAATGACCAAGTTCTCTTCATTGACCAA
NM_001243211.1      CTTGAATCTAAATTATCAGTCATAAGAAATTTGAATGACCAAGTTCTCTTCATTGACCAA
                    ************************************************************

HsCD00000423        GGAAATCGGCCTCTATTTGAAGATATGACTGATTCTGACTGTAGAGATAATGCACCCCGG
NM_001562.3         GGAAATCGGCCTCTATTTGAAGATATGACTGATTCTGACTGTAGAGATAATGCACCCCGG
NM_001243211.1      GGAAATCGGCCTCTATTTGAAGATATGACTGATTCTGACTGTAGAGATAATGCACCCCGG
                    ************************************************************

HsCD00000423        ACCATATTTATTATAAGTATGTATAAAGATAGCCAGCCTAGAGGTATGGCTGTAACTATC
NM_001562.3         ACCATATTTATTATAAGTATGTATAAAGATAGCCAGCCTAGAGGTATGGCTGTAACTATC
NM_001243211.1      ACCATATTTATTATAAGTATGTATAAAGATAGCCAGCCTAGAGGTATGGCTGTAACTATC
                    ************************************************************

HsCD00000423        TCTGTGAAGTGTGAGAAAATTTCAACTCTCTCCTGTGAGAACAAAATTATTTCCTTTAAG
NM_001562.3         TCTGTGAAGTGTGAGAAAATTTCAACTCTCTCCTGTGAGAACAAAATTATTTCCTTTAAG
NM_001243211.1      TCTGTGAAGTGTGAGAAAATTTCAACTCTCTCCTGTGAGAACAAAATTATTTCCTTTAAG
                    ************************************************************

HsCD00000423        GAAATGAATCCTCCTGATAACATCAAGGATACAAAAAGTGACATCATATTCTTTCAGAGA
NM_001562.3         GAAATGAATCCTCCTGATAACATCAAGGATACAAAAAGTGACATCATATTCTTTCAGAGA
NM_001243211.1      GAAATGAATCCTCCTGATAACATCAAGGATACAAAAAGTGACATCATATTCTTTCAGAGA
                    ************************************************************

HsCD00000423        AGTGTCCCAGGACATGATAATAAGATGCAATTTGAATCTTCATCATACGAAGGATACTTT
NM_001562.3         AGTGTCCCAGGACATGATAATAAGATGCAATTTGAATCTTCATCATACGAAGGATACTTT
NM_001243211.1      AGTGTCCCAGGACATGATAATAAGATGCAATTTGAATCTTCATCATACGAAGGATACTTT
                    ************************************************************

HsCD00000423        CTAGCTTGTGAAAAAGAGAGAGACCTTTTTAAACTCATTTTGAAAAAAGAGGATGAATTG
NM_001562.3         CTAGCTTGTGAAAAAGAGAGAGACCTTTTTAAACTCATTTTGAAAAAAGAGGATGAATTG
NM_001243211.1      CTAGCTTGTGAAAAAGAGAGAGACCTTTTTAAACTCATTTTGAAAAAAGAGGATGAATTG
                    ************************************************************

HsCD00000423        GGGGATAGATCTATAATGTTCACTGTTCAAAACGAAGACTTG
NM_001562.3         GGGGATAGATCTATAATGTTCACTGTTCAAAACGAAGACTAG
NM_001243211.1      GGGGATAGATCTATAATGTTCACTGTTCAAAACGAAGACTAG
                    ****************************************:*

Pathways

NCI : Cellular roles of Anthrax toxin
NCI : IL12 signaling mediated by STAT4
NCI : IL12-mediated signaling events
NCI : IL23-mediated signaling events
NCI : IL27-mediated signaling events
Panther : Interleukin signaling pathway
Panther : Toll receptor signaling pathway
Reactome : Cytokine Signaling in Immune system
Reactome : Immune System
Reactome : Interleukin-1 processing
Reactome : Signaling by Interleukins
WikiPathway : Hypertrophy Model
WikiPathway : NOD pathway

Protein

UniProt : Q14116
HPRD : 02976

Clone

Cloning Information : 21713
HIP Master Clone ID : 40273
Original Clone ID : FLH021713.01L

Organism


TAX_ID : 9606
Species Specific ID: 3606

Genome

Chromosome : 11
Map Location : 11q22.2-q22.3
Ensembl : ENSG00000150782

Reagents

Labome : IL-18-antibody