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Detailed Plasmid Information

TOP

Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: 9252h1BCt12p1
Keyword: full-length cds
Species: Unknown
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified.
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 18442260
Title: A common catalytic mechanism for proteins of the HutI family
Authors: PSI
New York SGX Research Center for Structural Genomics
UnCD00430274

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1242nts         Open reading frame : 51 to 1292

TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAATTGCGAAC
GTGTGTGGCTGAACGTTACCCCGGCGACGTTACGCAGCGATTTGGCCGACTATGGCCTTC
TCGAGCCACATGCATTAGGGGTCCACGAAGGGCGTATTCATGCTCTGGTACCTATGCAGG
ATTTAAAAGGACCCTACCCGGCGCACTGGCAAGACATGAAGGGCAAATTGGTGACTCCAG
GTCTTATCGATTGTCATACACACCTGATATTTGCCGGGTCTCGGGCAGAGGAATTTGAGC
TCCGCCAGAAAGGAGTTCCTTATGCTGAGATTGCGAGAAAGGGCGGTGGCATCATTAGTA
CCGTCCGAGCCACGCGTGCAGCTTCAGAAGACCAACTGTTCGAATTGGCGTTGCCGCGCG
TAAAGTCCTTGATCCGGGAAGGTGTGACTACAGTTGAAATAAAATCGGGGTATGGTCTGA
CCCTGGAGGATGAACTCAAGATGTTACGTGTCGCCCGCAGACTGGGCGAGGCTCTCCCAA
TTCGCGTTAAAACGACCCTTCTGGCTGCGCATGCCGTACCGCCAGAATATCGTGATGACC
CTGATAGCTGGGTTGAAACTATCTGCCAGGAGATTATACCGGCAGCGGCTGAAGCCGGTC
TTGCTGATGCGGTGGATGTATTCTGTGAGCACATCGGCTTTTCTCTGGCTCAGACAGAAC
AAGTTTACCTCGCCGCAGACCAGTACGGATTAGCGGTAAAAGGGCATATGGACCAATTAA
GTAATCTTGGAGGCTCAACGCTGGCTGCCAACTTCGGTGCATTATCCGTGGATCACCTGG
AATACCTCGACCCCGAGGGGATTCAGGCGTTGGCCCATCGCGGCGTCGTTGCAACCCTGC
TTCCGACTGCGTTTTATTTCTTAAAGGAAACAAAACTGCCACCTGTGGTAGCTCTGCGGA
AAGCCGGTGTCCCGATGGCGGTTTCGAGCGATATCAATCCAGGAACCGCTCCGATTGTGT
CTCTCCGTATGGCAATGAACATGGCCTGCACGTTGTTTGGCCTTACCCCCGTAGAAGCGA
TGGCTGGTGTTACTCGCCACGCAGCCCGTGCTCTTGGTGAGCAGGAACAACTGGGCCAGC
TGCGCGTGGGTATGTTAGCCGACTTCTTGGTCTGGAATTGTGGACATCCTGCAGAGTTAA
GTTATCTGATAGGCGTTGATCAGCTTGTGTCAAGAGTAGTCAACGGTGAAGAAACACTGC
ATGGGGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
c85t,g87a,L29L;t90g,G30G;
a249g,E83E;t351c,F117F;
g354a,E118E;a357g,L119L;
c361t,L121L;a375g,K125K;
c423t,Y141Y;a426t,G142G;
t429g,L143L;a483t,A161A;
t484c,g486c,L162L;c489a,P163P;
a495c,R165R;g498t,V166V;
t610c,a612t,L204L;a615t,A205A;
c618t,D206D;c630a,V210V;
t696c,Y232Y;t699a,G233G;
c700t,g702a,L234L;t723c,D241D;
g729a,L243L
                                

5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9252h
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 


Cloning Information: 496847
GI: 44384953
HIP Master Clone ID: 410527
Original Clone ID: 9252h1BCt12p1
Other Link: SBKB Structure Highlight
PDB - Structural Annotation: 2OOF
PDB - Structural Annotation: 2Q09
Structural Biology Knowledgebase: SBKB (2OOF)
Structural Biology Knowledgebase: SBKB (2Q09)
TargetTrack - Experimental data: NYSGXRC-9252h
Species Specific ID: None
Target GenBank: None
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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