Gene:
Gene Symbol:
Ucp2
Gene Name:
uncoupling protein 2 (mitochondrial, proton carrier)
Original Clone ID:
MPSbyNMR-002
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pET23b
Format:
FUSION
Source:
Membrane Protein Structures by Solution NMR
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 21785437
Title: Mitochondrial uncoupling protein 2 structure determined by NMR molecular fragment searching.
Title: Mitochondrial uncoupling protein 2 structure determined by NMR molecular fragment searching.
Authors:
Harvard Medical School
James Chou Lab
PSI:Biology
Membrane Protein Structures by Solution NMR
James Chou Lab
PSI:Biology
Membrane Protein Structures by Solution NMR
MmCD00433656
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 867nts
Open reading frame: 1 to 867
ATGACGGCCACGGTGAAATTCCTGGGCGCAGGCACGGCAGCAACCATCGCAGACCTGATT
ACGTTCCCGCTGGATACGGCGAAAGTCCGTCTGCAGATTCAAGGCGAATCACAGGGTCTG
GTTCGTACCGCAGCATCGGCACAATATCGTGGTGTCCTGGGTACCATCCTGACGATGGTG
CGTACGGAAGGCCCGCGCAGTCTGTACAATGGCCTGGTGGCTGGTCTGCAGCGTCAAATG
AGCTTTGCGAGCGTGCGCATTGGCCTGTATGATAGCGTCAAACAGTTCTACACCAAAGGT
TCTGAACATGCAGGTATCGGTAGCCGTCTGCTGGCAGGTTCTACCACGGGTGCACTGGCT
GTTGCAGTCGCACAGCCGACCGACGTGGTTAAAGTTCGTTTTCAGGCACAAGCTCGCGCG
GGCGGTCGTCGCTATCAAAGCACCGTGGAAGCTTACAAAACGATTGCGCGTGAAGAAGGC
ATCCGCGGTCTGTGGAAAGGTACCAGTCCGAACGTCGCCCGCAATGCAATTGTGAACTCC
GCAGAACTGGTTACCTATGATCTGATCAAAGACACGCTGCTGAAAGCGAATCTGATGACC
GATGACCTGCCGAGTCACTTTACGTCCGCTTTCGGCGCGGGTTTTAGTACCACGGTGATT
GCGTCCCCGGTTGATGTCGTGAAAACCCGTTATATGAACTCTGCCCTGGGCCAGTACCAT
TCAGCCGGTCACTCGGCACTGACGATGCTGCGTAAAGAAGGCCCGCGTGCGTTCTACAAA
GGTTTTATGCCGAGCTTCCTGCGCCTGGGTAGCTGGAATGTCGTGATGTTCGTGACCTAT
GAACAACTGAAACGCGCACTGATGGCG
Protein sequence predicted based on nucleotide sequence
MTATVKFLGAGTAATIADLITFPLDTAKVRLQIQGESQGLVRTAASAQYRGVLGTILTMV RTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHAGIGSRLLAGSTTGALA VAVAQPTDVVKVRFQAQARAGGRRYQSTVEAYKTIAREEGIRGLWKGTSPNVARNAIVNS AELVTYDLIKDTLLKANLMTDDLPSHFTSAFGAGFSTTVIASPVDVVKTRYMNSALGQYH SAGHSALTMLRKEGPRAFYKGFMPSFLRLGSWNVVMFVTYEQLKRALMA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MPSbyNMR-002
Recommended expression in:
Not Applicable
Vector Name:
pET23b
Synonyms:
pET-23b, pET23
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter, adds N-terminal T7 tag and C-terminal His tag; amp resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
3666
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (XhoI, NotI, EagI, HindIII, SalI, HincII, SacI, EcoRI, BamHI)
159
203
bacterial origin
ori
bacterial origin of replication
1430
2112
promoter
T7
T7 promoter
303
319
selectable marker
AmpR
ampicillin resistance gene
2211
3068
ssDNA origin
f1 ori
f1 origin
3200
3655
tag
T7 tag
T7 tag cds
207
239
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
