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NMI (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NMI
Gene Name:  N-myc (and STAT) interactor
Sequence             
Original Clone ID: ccsbBroad304_07362
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 24367263
Title: A Human Torque Teno Virus Encodes a MicroRNA That Inhibits Interferon Signaling
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00433976

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 922nts         Open reading frame : 1 to 922
Reference Sequence Alignment

ATGGAAGCTGATAAAGATGACACACAACAAATTCTTAAGGAGCATTCGCCAGATGAATTT
ATAAAAGATGAACAAAATAAGGGACTAATTGATGAAATTACAAAGAAAAATATTCAGCTA
AGGAAGGAGATCCAAAAGCTTGAAACGGAGTTACAAGAGGCTACCAAAGAATTCCAGATT
AAAGAGGATATTCCTGAAACAAAGATGAAATTCTTATCAGTTGAAACTCCTGAGAATGAC
AGCCAGTTGTCAAATATCTCCTGTTCGTTTCAAGTGAGCTCGAAAGTTCCTTATGAGATA
CAAAAAGGACAAGCACTTATCACCTTTGAAAAAGAAGAAGTTGCTCAAAATGTGGTAAGC
ATGAGTAAACATCATGTACAGATAAAAGATGTAAATCTGGAGGTTACGGCCAAGCCAGTT
CCATTAAATTCAGGAGTCAGATTCCAGGTTTATGTAGAAGTTTCTAAAATGAAAATCAAT
GTTACTGAAATTCCTGACACACTGCGTGAAGATCAAATGAGAGACAAACTAGAGCTGAGC
TTTTCAAAGTCCCGAAATGGAGGCGGAGAGGTGGACCGCGTGGACTATGACAGACAGTCC
GGGAGTGCAGTCATCACGTTTGTGGAGATTGGAGTGGCTGACAAGATTTTGAAAAAGAAA
GAATACCCTCTTTATATAAATCAAACTTGCCATAGAGTTACTGTTTCTCCATACACAGAA
ATACACTTGAAAAAGTATCAGATATTTTCAGGAACATCTAAGAGGACAGTGCTTCTGACA
GGAATGGAAGGCATTCAAATGGATGAAGAAATTGTGGAGGATTTAATTAACATTCACTTT
CAACGGGCAAAGAATGGAGGTGGAGAAGTAGATGTGGTCAAGTGTTCTCTAGGTCAACCT
CACATAGCATACTTTGAAGAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NMI
Symbol Nomenclature : NMI
Designation : N-myc interactor|N-myc-interactor
Full Nomenclature : N-myc (and STAT) interactor
GENEID : 9111
HGNC : 7854
MIM : 603525
Vega : OTTHUMG00000131867
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00433976        ATGGAAGCTGATAAAGATGACACACAACAAATTCTTAAGGAGCATTCGCCAGATGAATTT
XM_005246941.1      ATGGAAGCTGATAAAGATGACACACAACAAATTCTTAAGGAGCATTCGCCAGATGAATTT
NM_004688.2         ATGGAAGCTGATAAAGATGACACACAACAAATTCTTAAGGAGCATTCGCCAGATGAATTT
                    ************************************************************

HsCD00433976        ATAAAAGATGAACAAAATAAGGGACTAATTGATGAAATTACAAAGAAAAATATTCAGCTA
XM_005246941.1      ATAAAAGATGAACAAAATAAGGGACTAATTGATGAAATTACAAAGAAAAATATTCAACTA
NM_004688.2         ATAAAAGATGAACAAAATAAGGGACTAATTGATGAAATTACAAAGAAAAATATTCAACTA
                    ********************************************************.***

HsCD00433976        AGGAAGGAGATCCAAAAGCTTGAAACGGAGTTACAAGAGGCTACCAAAGAATTCCAGATT
XM_005246941.1      AAGAAGGAGATCCAAAAGCTTGAAACGGAGTTACAAGAGGCTACCAAAGAATTCCAGATT
NM_004688.2         AAGAAGGAGATCCAAAAGCTTGAAACGGAGTTACAAGAGGCTACCAAAGAATTCCAGATT
                    *.**********************************************************

HsCD00433976        AAAGAGGATATTCCTGAAACAAAGATGAAATTCTTATCAGTTGAAACTCCTGAGAATGAC
XM_005246941.1      AAAGAGGATATTCCTGAAACAAAGATGAAATTCTTATCAGTTGAAACTCCTGAGAATGAC
NM_004688.2         AAAGAGGATATTCCTGAAACAAAGATGAAATTCTTATCAGTTGAAACTCCTGAGAATGAC
                    ************************************************************

HsCD00433976        AGCCAGTTGTCAAATATCTCCTGTTCGTTTCAAGTGAGCTCGAAAGTTCCTTATGAGATA
XM_005246941.1      AGCCAGTTGTCAAATATCTCCTGTTCGTTTCAAGTGAGCTCGAAAGTTCCTTATGAGATA
NM_004688.2         AGCCAGTTGTCAAATATCTCCTGTTCGTTTCAAGTGAGCTCGAAAGTTCCTTATGAGATA
                    ************************************************************

HsCD00433976        CAAAAAGGACAAGCACTTATCACCTTTGAAAAAGAAGAAGTTGCTCAAAATGTGGTAAGC
XM_005246941.1      CAAAAAGGACAAGCACTTATCACCTTTGAAAAAGAAGAAGTTGCTCAAAATGTGGTAAGC
NM_004688.2         CAAAAAGGACAAGCACTTATCACCTTTGAAAAAGAAGAAGTTGCTCAAAATGTGGTAAGC
                    ************************************************************

HsCD00433976        ATGAGTAAACATCATGTACAGATAAAAGATGTAAATCTGGAGGTTACGGCCAAGCCAGTT
XM_005246941.1      ATGAGTAAACATCATGTACAGATAAAAGATGTAAATCTGGAGGTTACGGCCAAGCCAGTT
NM_004688.2         ATGAGTAAACATCATGTACAGATAAAAGATGTAAATCTGGAGGTTACGGCCAAGCCAGTT
                    ************************************************************

HsCD00433976        CCATTAAATTCAGGAGTCAGATTCCAGGTTTATGTAGAAGTTTCTAAAATGAAAATCAAT
XM_005246941.1      CCATTAAATTCAGGAGTCAGATTCCAGGTTTATGTAGAAGTTTCTAAAATGAAAATCAAT
NM_004688.2         CCATTAAATTCAGGAGTCAGATTCCAGGTTTATGTAGAAGTTTCTAAAATGAAAATCAAT
                    ************************************************************

HsCD00433976        GTTACTGAAATTCCTGACACACTGCGTGAAGATCAAATGAGAGACAAACTAGAGCTGAGC
XM_005246941.1      GTTACTGAAATTCCTGACACATTGCGTGAAGATCAAATGAGAGACAAACTAGAGCTGAGC
NM_004688.2         GTTACTGAAATTCCTGACACATTGCGTGAAGATCAAATGAGAGACAAACTAGAGCTGAGC
                    ********************* **************************************

HsCD00433976        TTTTCAAAGTCCCGAAATGGAGGCGGAGAGGTGGACCGCGTGGACTATGACAGACAGTCC
XM_005246941.1      TTTTCAAAGTCCCGAAATGGAGGCGGAGAGGTGGACCGCGTGGACTATGACAGACAGTCC
NM_004688.2         TTTTCAAAGTCCCGAAATGGAGGCGGAGAGGTGGACCGCGTGGACTATGACAGACAGTCC
                    ************************************************************

HsCD00433976        GGGAGTGCAGTCATCACGTTTGTGGAGATTGGAGTGGCTGACAAGATTTTGAAAAAGAAA
XM_005246941.1      GGGAGTGCAGTCATCACGTTTGTGGAGATTGGAGTGGCTGACAAGATTTTGAAAAAGAAA
NM_004688.2         GGGAGTGCAGTCATCACGTTTGTGGAGATTGGAGTGGCTGACAAGATTTTGAAAAAGAAA
                    ************************************************************

HsCD00433976        GAATACCCTCTTTATATAAATCAAACTTGCCATAGAGTTACTGTTTCTCCATACACAGAA
XM_005246941.1      GAATACCCTCTTTATATAAATCAAACCTGCCATAGAGTTACTGTTTCTCCATACACAGAA
NM_004688.2         GAATACCCTCTTTATATAAATCAAACCTGCCATAGAGTTACTGTTTCTCCATACACAGAA
                    ************************** *********************************

HsCD00433976        ATACACTTGAAAAAGTATCAGATATTTTCAGGAACATCTAAGAGGACAGTGCTTCTGACA
XM_005246941.1      ATACACTTGAAAAAGTATCAGATATTTTCAGGAACATCTAAGAGGACAGTGCTTCTGACA
NM_004688.2         ATACACTTGAAAAAGTATCAGATATTTTCAGGAACATCTAAGAGGACAGTGCTTCTGACA
                    ************************************************************

HsCD00433976        GGAATGGAAGGCATTCAAATGGATGAAGAAATTGTGGAGGATTTAATTAACATTCACTTT
XM_005246941.1      GGAATGGAAGGCATTCAAATGGATGAAGAAATTGTGGAGGATTTAATTAACATTCACTTT
NM_004688.2         GGAATGGAAGGCATTCAAATGGATGAAGAAATTGTGGAGGATTTAATTAACATTCACTTT
                    ************************************************************

HsCD00433976        CAACGGGCAAAGAATGGAGGTGGAGAAGTAGATGTGGTCAAGTGTTCTCTAGGTCAACCT
XM_005246941.1      CAACGGGCAAAGAATGGAGGTGGAGAAGTAGATGTGGTCAAGTGTTCTCTAGGTCAACCT
NM_004688.2         CAACGGGCAAAGAATGGAGGTGGAGAAGTAGATGTGGTCAAGTGTTCTCTAGGTCAACCT
                    ************************************************************

HsCD00433976        CACATAGCATACTTTGAAGAA---
XM_005246941.1      CACATAGCATACTTTGAAGAATAG
NM_004688.2         CACATAGCATACTTTGAAGAATAG
                    *********************   

Pathways

WikiPathway : IL-2 Signaling pathway

Protein

HPRD : 04632

Clone

Original Clone ID : ccsbBroad304_07362

Organism


TAX_ID : 9606
Species Specific ID: 9111

Genome

Chromosome : 2
Map Location : 2q23
Ensembl : ENSG00000123609

Reagents

Labome : NMI-antibody