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ZCRB1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZCRB1
Gene Name:  zinc finger CCHC-type and RNA binding motif 1
Sequence             
Original Clone ID: ccsbBroad304_09264
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00434449

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 652nts         Open reading frame : 1 to 652
Reference Sequence Alignment

ATGAGTGGTGGATTGGCTCCAAGTAAGAGCACAGTGTATGTATCCAACTTGCCTTTTTCC
CTGACAAACAATGACTTGTACCGGATATTTTCCAAGTATGGCAAAGTTGTAAAGGTTACC
ATCATGAAAGATAAAGATACCAGGAAGAGTAAAGGGGTTGCATTTATTTTATTTTTGGAT
AAAGACTCTGCACAAAACTGTACCAGGGCAATAAACAACAAACAGTTATTTGGTAGAGTG
ATAAAAGCAAGCATTGCTATTGACAATGGAAGAGCAGCTGAGTTCATCCGAAGGCGAAAC
TACTTTGATAAATCTAAGTGTTATGAATGTGGGGAAAGTGGACACTTAAGTTATGCCTGT
CCGAAAAATATGCTCGGAGAACGTGAGCCTCAAAAGAAGAAAGAAAAAAAGAAAAAAAAG
AAAGCTCCTGAACCAGAAGAAGAAATTGAGGAAGTAGAAGAAAGTGAAGATGAAGGGGAG
GATCCTGCTCTTGACAGCCTCAGTCAGGCCATAGCATTCCAGCAAGCCAAAATTGAAGAA
GAACAAAAAAAATGGAAACCCAGTTCAGGAGTCCCCTCAACATCAGATGATTCAAGACGC
CCAAGGATAAAGAAAAGCACATATTTCAGTGATGAGGAAGAACTTAGTGAT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZCRB1
Symbol Nomenclature : ZCRB1
SYNONYM : MADP-1; MADP1; RBM36; SNRNP31; ZCCHC19
Designation : U11/U12 small nuclear ribonucleoprotein 31 kDa protein|U11/U12 snRNP 31 kDa protein|U11/U12 snRNP 31K|U11/U12-31K|zinc finger CCHC-type and RNA-binding motif-containing protein 1
Full Nomenclature : zinc finger CCHC-type and RNA binding motif 1
GENEID : 85437
GenBank Accession : DQ895284
HGNC : 29620
MIM : 610750
Vega : OTTHUMG00000169382
Target GenBank: DQ895284

 

Reference Sequence Alignment

  

HsCD00434449      ATGAGTGGTGGATTGGCTCCAAGTAAGAGCACAGTGTATGTATCCAACTTGCCTTTTTCC
NM_033114.3       ATGAGTGGTGGATTGGCTCCAAGTAAGAGCACAGTGTATGTATCCAACTTGCCTTTTTCC
                  ************************************************************

HsCD00434449      CTGACAAACAATGACTTGTACCGGATATTTTCCAAGTATGGCAAAGTTGTAAAGGTTACC
NM_033114.3       CTGACAAACAATGACTTGTACCGGATATTTTCCAAGTATGGCAAAGTTGTAAAGGTTACC
                  ************************************************************

HsCD00434449      ATCATGAAAGATAAAGATACCAGGAAGAGTAAAGGGGTTGCATTTATTTTATTTTTGGAT
NM_033114.3       ATCATGAAAGATAAAGATACCAGGAAGAGTAAAGGGGTTGCATTTATTTTATTTTTGGAT
                  ************************************************************

HsCD00434449      AAAGACTCTGCACAAAACTGTACCAGGGCAATAAACAACAAACAGTTATTTGGTAGAGTG
NM_033114.3       AAAGACTCTGCACAAAACTGTACCAGGGCAATAAACAACAAACAGTTATTTGGTAGAGTG
                  ************************************************************

HsCD00434449      ATAAAAGCAAGCATTGCTATTGACAATGGAAGAGCAGCTGAGTTCATCCGAAGGCGAAAC
NM_033114.3       ATAAAAGCAAGCATTGCTATTGACAATGGAAGAGCAGCTGAGTTCATCCGAAGGCGAAAC
                  ************************************************************

HsCD00434449      TACTTTGATAAATCTAAGTGTTATGAATGTGGGGAAAGTGGACACTTAAGTTATGCCTGT
NM_033114.3       TACTTTGATAAATCTAAGTGTTATGAATGTGGGGAAAGTGGACACTTAAGTTATGCCTGT
                  ************************************************************

HsCD00434449      CCGAAAAATATGCTCGGAGAACGTGAGCCTCAAAAGAAGAAAGAAAAAAAGAAAAAAAAG
NM_033114.3       CCGAAAAATATGCTCGGAGAACGTGAGCCTCCAAAGAAGAAAGAAAAAAAGAAAAAAAAG
                  *******************************.****************************

HsCD00434449      AAAGCTCCTGAACCAGAAGAAGAAATTGAGGAAGTAGAAGAAAGTGAAGATGAAGGGGAG
NM_033114.3       AAAGCTCCTGAACCAGAAGAAGAAATTGAGGAAGTAGAAGAAAGTGAAGATGAAGGGGAG
                  ************************************************************

HsCD00434449      GATCCTGCTCTTGACAGCCTCAGTCAGGCCATAGCATTCCAGCAAGCCAAAATTGAAGAA
NM_033114.3       GATCCTGCTCTTGACAGCCTCAGTCAGGCCATAGCATTCCAGCAAGCCAAAATTGAAGAA
                  ************************************************************

HsCD00434449      GAACAAAAAAAATGGAAACCCAGTTCAGGAGTCCCCTCAACATCAGATGATTCAAGACGC
NM_033114.3       GAACAAAAAAAATGGAAACCCAGTTCAGGAGTCCCCTCAACATCAGATGATTCAAGACGC
                  ************************************************************

HsCD00434449      CCAAGGATAAAGAAAAGCACATATTTCAGTGATGAGGAAGAACTTAGTGAT---
NM_033114.3       CCAAGGATAAAGAAAAGCACATATTTCAGTGATGAGGAAGAACTTAGTGATTAA
                  ***************************************************   

Protein

SMART domain : RRM_1 : RNA recognition motif
SMART domain : ZnF_C2HC : zinc finger
SMART domain : RRM : RNA recognition motif
HPRD : 14344

Clone

Original Clone ID : ccsbBroad304_09264

Organism


TAX_ID : 9606
Species Specific ID: 85437

Genome

Chromosome : 12
Map Location : 12q12
Ensembl : ENSG00000139168

Reagents

Labome : ZCRB1-antibody