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ZBP1 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZBP1
Gene Name:  Z-DNA binding protein 1
Sequence             
Original Clone ID: ccsbBroad304_14288
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00434653

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 447nts         Open reading frame : 1 to 447

ATGGCCCAGGCTCCTGCTGACCCGGGCAGAGAAGGCCACCTTGAACAAAGAATCCTGCAG
GTGCTGACAGAGGCTGGCTCCCCGGTGAAACTTGCCCAGCTGGTGAAGGAATGCCAAGCA
CCCAAGAGGGAGCTCAACCAAGTCCTCTACCGAATGAAAAAGGAGTTGAAAGTCTCCCTC
ACATCCCCTGCCACCTGGTGCTTGGGCGGGACTGATCCTGAAGGCGAGGGTCCTGCAGAG
CTGGCCTTGTCCAGCCCTGGTAACTGCCACCCCGGGGAGGCGGGTCTGACCCTGCAGGGA
GCATCCTGGCAATGGACAAGCACAGATTTGAGCCTGGGTTCGAATCTGAACTCTGCCACA
TGGGAGCTCACAGGTTTCCTCTCTCTGTGCCTTGGTTTCTTTTTCTGGTTGATGGAGCTC
ACAGCAGGGCTGCTTGGTAGGGTTGC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
3 errors; 1 non-synonomous errors;
 insertion or deletion in coding sequence
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZBP1
Symbol Nomenclature : ZBP1
SYNONYM : C20orf183; DAI; DLM-1; DLM1
Designation : DNA-dependent activator of IFN-regulatory factors|DNA-dependent activator of IRFs|DNA-dependent activator of interferon regulatory factors|Z-DNA-binding protein 1|tumor stroma and activated macrophage protein DLM-1
Full Nomenclature : Z-DNA binding protein 1
GENEID : 81030
Locus Tag : RP4-718J7.1
HGNC : 16176
MIM : 606750
Vega : OTTHUMG00000032824
Target GenBank: None

 

Pathways

Reactome : Cytosolic sensors of pathogen-associated DNA
Reactome : IRF3 mediated activation of type 1 IFN
Reactome : IRF3-mediated induction of type I IFN
Reactome : Immune System
Reactome : Innate Immune System
Reactome : RIP-mediated NFkB activation via ZBP1
Reactome : Regulation of innate immune responses to cytosolic DNA
Reactome : STING mediated induction of host immune responses
Reactome : ZBP1(DAI) mediated induction of type I IFNs

Clone

Original Clone ID : ccsbBroad304_14288

Organism


TAX_ID : 9606
Species Specific ID: 81030

Genome

Chromosome : 20
Map Location : 20q13.31
Ensembl : ENSG00000124256

Reagents

Labome : DLM-1-antibody